CAJ | 학술논문

目的观察巨噬细胞对胃癌细胞生物学行为的影响,以探讨肿瘤相关性巨噬细胞(TAMs)在胃癌侵袭转移中的作用.方法将人单核细胞株THP-1体外诱导为巨噬细胞,并与胃癌细胞株AGS共培养模拟胃癌微环境,观察并分析其对胃癌细胞增殖力和侵袭力的影响.结果胃癌细胞与巨噬细胞共培养后,增殖能力有所减弱,倍增时间为(38.42±2.68)h,而未经共培养者倍增时间为(24.36±2.91) h(P ＜0.001)；而体外侵袭实验显示胃癌细胞共培养后及未共培养时侵袭细胞计数分别为308±15和168±4,侵袭力增强(P＜0.01).巨噬细胞对胃癌细胞的作用与其分泌细胞因子相关.THP-1细胞激活并分化为巨噬细胞后,培养上清中白细胞介素(IL)-8及IL-1β表达量分别增加了40和20倍(P＜0.01)；肿瘤坏死因子(TNF)-α表达量增加了8倍(P＞0.05).结论巨噬细胞可增强胃癌细胞的侵袭力,该作用可能与其分泌的细胞因子IL-8及IL-1β和TNF-α增高有关.
목적 관찰거서세포대위암세포생물학행위적영향,이탐토종류상관성거서세포(TAMs)재위암침습전이중적작용.방법 장인단핵세포주THP-1체외유도위거서세포,병여위암세포주AGS공배양모의위암미배경,관찰병분석기대위암세포증식력화침습력적영향.결과 위암세포여거서세포공배양후,증식능력유소감약,배증시간위(38.42±2.68)h,이미경공배양자배증시간위(24.36±2.91) h(P ＜0.001)；이체외침습실험현시위암세포공배양후급미공배양시침습세포계수분별위308±15화168±4,침습력증강(P＜0.01).거서세포대위암세포적작용여기분비세포인자상관.THP-1세포격활병분화위거서세포후,배양상청중백세포개소(IL)-8급IL-1β표체량분별증가료40화20배(P＜0.01)；종류배사인자(TNF)-α표체량증가료8배(P＞0.05).결론 거서세포가증강위암세포적침습력,해작용가능여기분비적세포인자IL-8급IL-1β화TNF-α증고유관.
Objective To observe the biological effects on human gastric cancer cell line AGS exerted by macrophages and discuss the role of tumor-associated macrophages (TAMs) on gastric cancer invasion and metastasis.Methods Human monocytic cell line THP-1 were differentiated to macrophages in vitro,then cocultured with AGS cells to mimic the micro-environment of gastric cancer.The effects on proliferation and invasiveness activities of gastric cancer cells initiated by macrophages were assessed.Results The AGS cells cocultured with macrophages were shown decreased proliferation,and the cell doubhng time was (38.42 ± 2.68) h,which was (24.36 ± 2.91) h in those non-cocuhured (P ＜ 0.001).In vitro invasion assay indicated that the invasive cell count of AGS cells cocultured with macrophages and those noncocultured was 308 ± 15 and 168 ±4 separately (P ＜0.01).The effects of macrophages on gastric cancer cells were related to the cytokines.The THP-1derived macrophages secreted interleukin (IL)-1β 20 fold higher (P ＜0.01),IL-8 40 fold higher (P ＜0.01) and tumor necrosis factor (TNF)-α 8 fold higher (P ＞0.05) than those THP-1 cells did.Conclusion macrophages could enhance the invasiveness of gastric cancer cells,whith may ralated to their inceased secretion of IL-1β,IL-8 and TNF-α.