CAJ | 학술논문

目的应用比较蛋白组学研究胃癌腹膜转移形成相关的蛋白质分子标志物.方法以胃癌细胞株(BGC823)和腹膜高转移潜能的胃癌细胞株(SCG7901)为研究对象,采用双向凝胶电泳(2-DE)对其进行总蛋白的分离,两组之间的差异蛋白通过质谱分析和蛋白数据库搜索鉴定,并用Western blot法对差异蛋白点进行进一步检测验证.结果腹膜高转移潜能的胃癌细胞株(SCG7901)与胃癌细胞株(BGC823)比较,共鉴定出20个有意义的蛋白质,其中11个蛋白仅在SCG7901细胞株中表达明显增高,9个蛋白仅在BGC823细胞株中表达明显增高.对于SCG7901细胞株高表达的叉头蛋白3(Foxp3),应用Western blot法在蛋白水平上验证了2-DE结果.结论腹膜高转移潜能的胃癌细胞株(SCG7901)与胃癌细胞株(BGC823)的蛋白质表达差异有统计学意义.
목적 응용비교단백조학연구위암복막전이형성상관적단백질분자표지물.방법 이위암세포주(BGC823)화복막고전이잠능적위암세포주(SCG7901)위연구대상,채용쌍향응효전영(2-DE)대기진행총단백적분리,량조지간적차이단백통과질보분석화단백수거고수색감정,병용Western blot법대차이단백점진행진일보검측험증.결과 복막고전이잠능적위암세포주(SCG7901)여위암세포주(BGC823)비교,공감정출20개유의의적단백질,기중11개단백부재SCG7901세포주중표체명현증고,9개단백부재BGC823세포주중표체명현증고.대우SCG7901세포주고표체적차두단백3(Foxp3),응용Western blot법재단백수평상험증료2-DE결과.결론 복막고전이잠능적위암세포주(SCG7901)여위암세포주(BGC823)적단백질표체차이유통계학의의.
Objective To study the key proteins associated with metastasis of gastric cancer,and the potential diagnostic markers and therapeutic targets of this malignancy.Methods A comparative proteomic strategy,in a combination of two-dimensional electrophoresis separation and mass spectrometry identification,was adopted to search for proteome alternations in an gastric cancer mother cell line BGC823 and its highly peritoneal metastatic cell subline SCG7901.Results There were 20 significant proteins identified in total.Among the 20 spots,11 proteins were up-regulated in cell line SCG7901,whereas 9 were up-regulated in cell line BGC823.Western blotting confirmed the difference of forkhead box P3 (Foxp3) on protein level.Conclusion The results suggest that an obviously differential proteomic expression exists between the human gastric cancer mother cell line BGC823 and its highly peritoneal metastatic cell subline SCG7901.