CAJ | 학술논문

目的观察鱼藤素联合顺铂对入胃癌细胞株MGC-803的杀伤、增殖抑制作用.方法鱼藤素(1.56、3.12、6.25 mg/L)联合顺铂(0.78、1.56、3.12、6.25、12.50、25.00 mg/L)作用于MGC-803细胞,采用噻唑蓝(MTT)法及形态学观察检测细胞增殖抑制及凋亡诱导作用,应用等效线图分析协同效应及流式细胞术检测细胞周期分布.结果鱼藤素与顺铂联合作用48 h,顺铂的半数抑药浓度(IC50)分别为4.69、3.34、1.45 mg/L,较单独应用7.66 mg/L明显降低(P＜0.05),细胞的凋亡率明显增加,合用指数(CI)值小于1；1.56 mg/L的鱼藤素作用48 h后,MGC-803细胞G1期比例由28.1％增加至41.4％.结论鱼藤素与顺铂联合应用具有明显的抗肿瘤作用,且鱼藤素特异性的阻滞G1期细胞,并与顺铂产生协同作用.
목적 관찰어등소연합순박대입위암세포주MGC-803적살상、증식억제작용.방법 어등소(1.56、3.12、6.25 mg/L)연합순박(0.78、1.56、3.12、6.25、12.50、25.00 mg/L)작용우MGC-803세포,채용새서람(MTT)법급형태학관찰검측세포증식억제급조망유도작용,응용등효선도분석협동효응급류식세포술검측세포주기분포.결과 어등소여순박연합작용48 h,순박적반수억약농도(IC50)분별위4.69、3.34、1.45 mg/L,교단독응용7.66 mg/L명현강저(P＜0.05),세포적조망솔명현증가,합용지수(CI)치소우1；1.56 mg/L적어등소작용48 h후,MGC-803세포G1기비례유28.1％증가지41.4％.결론 어등소여순박연합응용구유명현적항종류작용,차어등소특이성적조체G1기세포,병여순박산생협동작용.
Objective To investigate the effect of deguelin combined with cisplatin in human gastric cancer cell line MGC-803.Methods The cytotoxicity of deguelin (1.56,3.12,6.25 mg/L)and cisplatin (0.78,1.56,3.12,6.25,12.50,25.00 mg/L) in MGC-803 cells was determined by the methyl thiazol tetrazolium (MTT) assay and further confirmed by morphological analysis.Interactions of combination between two drugs were analyzed by isobologram.Cell cycle was assayed by flow cytometry.Results Combination of deguelin and cisplatin exhibited synergistic inhibition of proliferation in MGC-803 cells (P ＜0.05),induced significant apoptosis and morphological changes.After exposure to deguelin (1.56 mg/L) for 48 h the cells at G1 phase increased.Conclusion Combination of deguelin and cisplatin showed synergistic antitumor effects in MGC-803 cells via cell cycle block at G1 phase.