CAJ | 학술논문

目的探讨UC001kfo对肝癌细胞侵袭的影响及调控目标是否为α-平滑肌肌动蛋白(α-SMA).方法以肝癌细胞HepG2为细胞模型,分为UC001 kfo RNA干扰组(UC001 kfo-siRNA组)和阴性对照组,每组3个复孔,实时定量逆转录聚合酶链反应(RT-qPCR)检测沉默效率,细胞划痕试验和Transwell小室试验分析HepG2侵袭转移能力,RT-qPCR检测α-SMA mRNA的表达,并将UC001kfo和α-SMA的表达量进行相关分析.结果划痕试验结果显示,阴性对照组、UC001 kfo-siRNA组48 h迁移距离分别为(17.55 ±0.44)um,UC001kfo-siRNA组(10.55±0.41) μm,差异有统计学意义(P ＜0.01)；Transwell小室侵袭试验结果显示,UC001kfo-siRNA组穿膜细胞数[(56±10)个]明显少于阴性对照组[(141±21)个,P＜0.01]；RT-qPCR结果显示UC001 kfo-siRNA组UC001 kfo相对阴性对照组的表达量为0.23 ±0.20,α-SMA的表达量为0.36 ±0.17,与阴性对照组比较两者表达差异有统计学意义(P＜0.05),相关分析表明α-SMA与UC001kfo的表达量呈正相关(r=0.997,P＜0.05).结论 UC001kfo通过调控α-SMA的表达促进肝癌细胞的迁移侵袭.
목적 탐토UC001kfo대간암세포침습적영향급조공목표시부위α-평활기기동단백(α-SMA).방법 이간암세포HepG2위세포모형,분위UC001 kfo RNA간우조(UC001 kfo-siRNA조)화음성대조조,매조3개복공,실시정량역전록취합매련반응(RT-qPCR)검측침묵효솔,세포화흔시험화Transwell소실시험분석HepG2침습전이능력,RT-qPCR검측α-SMA mRNA적표체,병장UC001kfo화α-SMA적표체량진행상관분석.결과 화흔시험결과현시,음성대조조、UC001 kfo-siRNA조48 h천이거리분별위(17.55 ±0.44)um,UC001kfo-siRNA조(10.55±0.41) μm,차이유통계학의의(P ＜0.01)；Transwell소실침습시험결과현시,UC001kfo-siRNA조천막세포수[(56±10)개]명현소우음성대조조[(141±21)개,P＜0.01]；RT-qPCR결과현시UC001 kfo-siRNA조UC001 kfo상대음성대조조적표체량위0.23 ±0.20,α-SMA적표체량위0.36 ±0.17,여음성대조조비교량자표체차이유통계학의의(P＜0.05),상관분석표명α-SMA여UC001kfo적표체량정정상관(r=0.997,P＜0.05).결론 UC001kfo통과조공α-SMA적표체촉진간암세포적천이침습.
Objective To study the impact of UC001kfo on the invasion of hepatocellular carcinoma cells and whether the regulation target is α-smooth muscle actin (α-SMA).Methods The hepatocellular carcinoma cell line HepG2 was used as the cell model,groups were divided into UC001kfo RNA interference group (UC001kfo-siRNA group) and negative control group.The RNA interference technique was performed to down-regulate the expression of UC001kfo,then the real-time reverse transcriptase-polymerase chain reaction (RT-qPCR) was used to examine the silence efficiency,the scratch adhesion test and transwell small cell test were used to analyze the invasiveness of HepG2,RT-qPCR was used to examine the expression of α-SMA mRNA,and the correlation analysis was conducted to the expressions of UC001kfo and α-SMA.Results The 48 h migration distances of the negative control group and UC001kfo-siRNA group were (17.55 ± 0.44) μm and (10.55 ± 0.41) μm respectively,and the difference has statistical significance (P ＜ 0.01) ; the number of transmembrane cells was (56 ± 10),which was significantly less than that of the negative control group of (141 ± 21) (P ＜ 0.01) ; the RT-qPCR result shows that the UC001kfo relative expression of the UC001kfo-siRNA group was 0.23 ± 0.20,the expression of α-SMA was 0.36 ±0.17,and compared to the negative control group,both of them have statistical significance (P ＜ 0.05).The correlation analysis shows that α-SMA has a positive correlation with the expression of UC001kfo (r =0.997,P ＜ 0.05).Conclusion UC001kfo can promote the migration and invasion of hepatocellular carcinoma cells by regulating α-SMA.