中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
2期
319-321
,共3页
万丽丽%夏家红%陈军%王刚
萬麗麗%夏傢紅%陳軍%王剛
만려려%하가홍%진군%왕강
缺血再灌注%槲皮素%还原型烟酰胺腺嘌呤二核苷酸磷酸%内皮型一氧化氮合酶%诱导型一氧化氮合酶
缺血再灌註%槲皮素%還原型煙酰胺腺嘌呤二覈苷痠燐痠%內皮型一氧化氮閤酶%誘導型一氧化氮閤酶
결혈재관주%곡피소%환원형연선알선표령이핵감산린산%내피형일양화담합매%유도형일양화담합매
Ischemia-reperfusion injury%Quercetin%Phagocytic nicotinamide adenine dinucleotide phosphate oxidase%Endothelial nitricoxide synthase%Inducible nitricoxide synthase
目的 观察槲皮素对兔缺血再灌注心肌还原型烟酰胺腺嘌呤二核苷酸磷酸(NOX2)、一氧化氮合酶(NOS)基因和蛋白表达的影响.方法 建立成年家兔心肌缺血再灌注模型,结扎左缘支30 min,再灌注12h.实验分4组:空白对照组(control)、缺血再灌注组(I/R)、槲皮素组(Que)、槲皮素+缺血再灌注组(I/R+ Que).采用实时定量聚合酶链反应(Real-time PCR)和Western blot方法检测心肌NOX2、内皮型一氧化氮合酶(eNOS)、诱导型一氧化氮合酶(iNOS) mRNA和蛋白的表达.结果 I/R组NOX2 mRNA和蛋白表达分别为1.73±0.41、3.46 ±0.84,iNOS mRNA和蛋白表达分别为0.29±0.11、0.29 ±0.11,较对照组明显增高(P＜0.01),eNOS变化不明显(P＜0.01).给予槲皮素后,Que组NOX2 mRNA和蛋白表达分别为0.40±0.08、0.56 ±0.28,eNOS mRNA和蛋白表达分别为0.22±0.07、0.95±0.23,iNOS mRNA和蛋白表达分别为0.02±0.01、0.87±0.31;I/R+ Que组NOX2 mRNA和蛋白表达分别为1.03 ±0.31、2.05 ±0.49,eNOS mRNA和蛋白表达分别为0.32±0.09、1.12±0.36,iNOS mRNA和蛋白表达分别为0.16 ±0.05、3.17±1.29;较对照组水平明显降低(P＜0.01).结论 心肌缺血再灌注后,NOX2、iNOS表达增加；槲皮素明显减少心肌缺血再灌注后NOX2、iNOS和eNOS的表达.
目的 觀察槲皮素對兔缺血再灌註心肌還原型煙酰胺腺嘌呤二覈苷痠燐痠(NOX2)、一氧化氮閤酶(NOS)基因和蛋白錶達的影響.方法 建立成年傢兔心肌缺血再灌註模型,結扎左緣支30 min,再灌註12h.實驗分4組:空白對照組(control)、缺血再灌註組(I/R)、槲皮素組(Que)、槲皮素+缺血再灌註組(I/R+ Que).採用實時定量聚閤酶鏈反應(Real-time PCR)和Western blot方法檢測心肌NOX2、內皮型一氧化氮閤酶(eNOS)、誘導型一氧化氮閤酶(iNOS) mRNA和蛋白的錶達.結果 I/R組NOX2 mRNA和蛋白錶達分彆為1.73±0.41、3.46 ±0.84,iNOS mRNA和蛋白錶達分彆為0.29±0.11、0.29 ±0.11,較對照組明顯增高(P＜0.01),eNOS變化不明顯(P＜0.01).給予槲皮素後,Que組NOX2 mRNA和蛋白錶達分彆為0.40±0.08、0.56 ±0.28,eNOS mRNA和蛋白錶達分彆為0.22±0.07、0.95±0.23,iNOS mRNA和蛋白錶達分彆為0.02±0.01、0.87±0.31;I/R+ Que組NOX2 mRNA和蛋白錶達分彆為1.03 ±0.31、2.05 ±0.49,eNOS mRNA和蛋白錶達分彆為0.32±0.09、1.12±0.36,iNOS mRNA和蛋白錶達分彆為0.16 ±0.05、3.17±1.29;較對照組水平明顯降低(P＜0.01).結論 心肌缺血再灌註後,NOX2、iNOS錶達增加；槲皮素明顯減少心肌缺血再灌註後NOX2、iNOS和eNOS的錶達.
목적 관찰곡피소대토결혈재관주심기환원형연선알선표령이핵감산린산(NOX2)、일양화담합매(NOS)기인화단백표체적영향.방법 건립성년가토심기결혈재관주모형,결찰좌연지30 min,재관주12h.실험분4조:공백대조조(control)、결혈재관주조(I/R)、곡피소조(Que)、곡피소+결혈재관주조(I/R+ Que).채용실시정량취합매련반응(Real-time PCR)화Western blot방법검측심기NOX2、내피형일양화담합매(eNOS)、유도형일양화담합매(iNOS) mRNA화단백적표체.결과 I/R조NOX2 mRNA화단백표체분별위1.73±0.41、3.46 ±0.84,iNOS mRNA화단백표체분별위0.29±0.11、0.29 ±0.11,교대조조명현증고(P＜0.01),eNOS변화불명현(P＜0.01).급여곡피소후,Que조NOX2 mRNA화단백표체분별위0.40±0.08、0.56 ±0.28,eNOS mRNA화단백표체분별위0.22±0.07、0.95±0.23,iNOS mRNA화단백표체분별위0.02±0.01、0.87±0.31;I/R+ Que조NOX2 mRNA화단백표체분별위1.03 ±0.31、2.05 ±0.49,eNOS mRNA화단백표체분별위0.32±0.09、1.12±0.36,iNOS mRNA화단백표체분별위0.16 ±0.05、3.17±1.29;교대조조수평명현강저(P＜0.01).결론 심기결혈재관주후,NOX2、iNOS표체증가；곡피소명현감소심기결혈재관주후NOX2、iNOS화eNOS적표체.
Objective Investigate the effects of quercetin on phagocytic nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX2),endothelial nitricoxide synthase (eNOS) and inducible nitricoxide synthase (iNOS) after myocardial ischemia-reperfusion injury (MIRI) in rabbit.Methods New Zealand rabbits were subjected to 30 minutes of myocardial ischemia followed by 12 hours of reperfusion.They then were randomly assigned to four experimental groups:control,L/R,quercetin (Que),I/R + Que.Gene and protein expression of NOX2,eNOS and iNOS were compared.Results mRNA and protein expression of NOX2 in I/R were 0.29 ± 0.11,0.29 ±0.11,mRNA and protein expression of iNOS in I/R were 0.29 ± 0.11,0.29 ± 0.11.Both in Real-time polymerase chain reaction (Real-time PCR) and Westem blotting studies,myocardial ischemia reperfusion-induced NOX2 and iNOS expression were enhanced (P ＜0.01),but eNOS mRNA and protein in I/R hearts were not significantly different from that in control (P＜0.01).After administration of quercetin,mRNA and protein expression of NOX2 in Que were 0.40 ± 0.08,0.56 ± 0.28,mRNA and protein expression of eNOS in Que were 0.22 ± 0.07,0.95 ±0.23,mRNA and protein expression of iNOS in Que were 0.02 ±0.01,0.87 ±0.31 ; mRNA and protein expression of NOX2 in I/R + Que were 1.03 ±0.31,2.05 ±0.49,mRNA and protein expression of eNOS in I/R + Que were 0.32 ± 0.09,1.12 ± 0.36,mRNA and protein expression of iNOS in I/R + Que were 0.16 ±0.05,3.17 ± 1.29.Administration of quercetin reduced NOX2,eNOS and iNOS mRNA and protein expression both in control and I/R heart (P ＜ 0.01).Conclusion Gene and protein expression of NOX2 and iNOS were increased after MIRI.Quercetin not only inhibited myocardial ischemia reperfusioninduced NOX2 and iNOS mRNA and protein expression but also eNOS mRNA and protein expression.
