CAJ | 학술논문

目的观察低浓度血清条件下不同浓度的血小板衍生因子BB(PDGFBB)和碱性成纤维细胞生长因子(bFGF)在人肌腱细胞体外培养中对人肌腱细胞的增殖和胶原形成的影响.方法在含0％和1％胎牛血清(FBS)的c-MEM培养基中,加入PDGFBB(5、10、50 μg/L),bFGF(5、10、50 μg/L)进行人肌腱细胞培养,10％ FBS的培养组作为对照组.使用拉马拉蓝测定细胞增殖速度及天狼猩红染色定量肌腱细胞胶原产生.结果培养基内加入50 μg/L PDGFBB+50 μg/L bFGF可以把FBS的使用量降至1％,并达到10％ FBS的增殖速率(约4倍增加),差异无统计学意义(P＞0.05)；50 μg/LPDGFB +50 μg/L bFGF明显抑制肌腱细胞胶原形成(0.211 ±0.002) ng,与对照组比较[(0.485±0.068) ng],差异有统计学意义(P＜0.05).结论使用50 μg/L PDGFBB+50 μg/L bFGF添加于α-MEM培养基,可以把FBS使用量降至1％,不但可以达到10％ FBS的增殖速率,还能够抑制肌腱细胞的胶原产生.
목적 관찰저농도혈청조건하불동농도적혈소판연생인자BB(PDGFBB)화감성성섬유세포생장인자(bFGF)재인기건세포체외배양중대인기건세포적증식화효원형성적영향.방법 재함0％화1％태우혈청(FBS)적c-MEM배양기중,가입PDGFBB(5、10、50 μg/L),bFGF(5、10、50 μg/L)진행인기건세포배양,10％ FBS적배양조작위대조조.사용랍마랍람측정세포증식속도급천랑성홍염색정량기건세포효원산생.결과 배양기내가입50 μg/L PDGFBB+50 μg/L bFGF가이파FBS적사용량강지1％,병체도10％ FBS적증식속솔(약4배증가),차이무통계학의의(P＞0.05)；50 μg/LPDGFB +50 μg/L bFGF명현억제기건세포효원형성(0.211 ±0.002) ng,여대조조비교[(0.485±0.068) ng],차이유통계학의의(P＜0.05).결론 사용50 μg/L PDGFBB+50 μg/L bFGF첨가우α-MEM배양기,가이파FBS사용량강지1％,불단가이체도10％ FBS적증식속솔,환능구억제기건세포적효원산생.
Objective To evaluate the effect of platelet derived growth factor (PDGF) BB and basic fibroblast growth factor (bFGF) on proliferation and collagen synthesis of tenocytes in in vitro culture.Methods Human tenocytes were cultured in α-MEM medium supplemented with fatal bovine serun (FBS) at various concentrations and both PDGFBB and bFGF.AlamarBlue was used to examine proleration of tenocytes,and Sirius red staining was employed to evaluate the collagen synthesis of the cells studied.Results The tenocytes cultured for 14 days with 1％ FBS + 50 μg/L PDGFBB +50 μg/L bFGF showed similar proliferation in comparison with those cultured in 10％ FBS (approximately 400％ increase).Tenocytes cultured in 50 μg/L PDGFBB + 50 μg/L bFGF showed significantly reduced collagen synthesis [(0.211 ±0.002) ng] in comparison with those cultured in 10％ FBS [(0.485 ±0.06g) ng].Conclusion These findings have demonstrated that in the presence of 50 μg/L PDGFBB + 50 μg/L bFGF in the culture medium,FBS usage can be reduced to 1％,which can not only obtain the same prolifertion rate as the 10％ FBS,but also inhibit collagen synthesis.