CAJ | 학술논문

目的观察骨质疏松对磨损颗粒诱导骨溶解的影响.方法将42只6月龄雌性SD大鼠随机分成A、B和C共3组,A组和B组各18只,C组6只.A组大鼠切除双侧卵巢；B组暂不处理；C组分离双侧卵巢,但不切除.饲养3个月后,从A组和B组中各随机选取6只及C组的全部大鼠,处死后行全身骨密度检查,并取右侧胫骨行显微CT(μCT)和骨形态计量学检查.A组中剩余的12只大鼠随机分成A1和A2组,每组6只,A1组大鼠颅骨正中矢状缝骨外膜区域,注入5 mg的金属钛颗粒悬浮液；在A2组植入等体积的磷酸盐缓冲液(PBS)；同法将B组分为B1和B2组.14d后处死所有剩余大鼠,对大鼠颅骨行μCT及病理切片以检测颅骨溶骨效应.结果与B组和C组比较,A组的骨密度和胫骨骨形态计量学指标显著降低(P＜0.05),胫骨μCT检查示骨小梁稀疏.A1组和A2组大鼠颅骨行μCT检查溶骨不明显,病理切片检查示A1、A2、B1和B2组矢状缝骨外膜区域内溶骨面积分别为(0.262 ±0.009)、(0.130±0.013)、(0.307 ±0.013)、(0.178 ±0.011) mm2,组间差异有统计学意义(P＜0.05).结论在动物模型中,骨质疏松能减缓磨损颗粒诱导的骨溶解过程.
목적 관찰골질소송대마손과립유도골용해적영향.방법 장42지6월령자성SD대서수궤분성A、B화C공3조,A조화B조각18지,C조6지.A조대서절제쌍측란소；B조잠불처리；C조분리쌍측란소,단불절제.사양3개월후,종A조화B조중각수궤선취6지급C조적전부대서,처사후행전신골밀도검사,병취우측경골행현미CT(μCT)화골형태계량학검사.A조중잉여적12지대서수궤분성A1화A2조,매조6지,A1조대서로골정중시상봉골외막구역,주입5 mg적금속태과립현부액；재A2조식입등체적적린산염완충액(PBS)；동법장B조분위B1화B2조.14d후처사소유잉여대서,대대서로골행μCT급병리절편이검측로골용골효응.결과 여B조화C조비교,A조적골밀도화경골골형태계량학지표현저강저(P＜0.05),경골μCT검사시골소량희소.A1조화A2조대서로골행μCT검사용골불명현,병리절편검사시A1、A2、B1화B2조시상봉골외막구역내용골면적분별위(0.262 ±0.009)、(0.130±0.013)、(0.307 ±0.013)、(0.178 ±0.011) mm2,조간차이유통계학의의(P＜0.05).결론 재동물모형중,골질소송능감완마손과립유도적골용해과정.
Objective To study the influence of osteoporosis (OP) on wear particles-induced osteolysis in an animal model.Methods Forty-two female Sprague-Dawley (SD) rats aged 6 months were randomly divided into 3 groups:A,B and C.Groups A and B contained 18 rats each,and group C contained 6 rats.The rats in group A were operated by bilateral ovariectomy,no operation was done on rats of group B,and that in group C was sham.After 3 months,6 rats in group A,6 rats in group B and all the rats in group C were harvested.Bone mineral density (BMD),μCT and bone histomorphometry were analyzed.The rest of rats in group A were randomly divided into two subgroups:subgroup A1 and subgroup A2,and the same as group B.Five mg titanium particles were implanted onto the calvaria of subgroups A1 and B1,and PBS was injected to subgroups A2 and B2.Calvarias were harvested after 14 days and analyzed by μCT,and histomorphometry was done to measure the osteolysis area of calvarial sagittal suture.Results As compared with groups B and C,BMD and bone histomorphometry index in A group were significantly reduced (P ＜ 0.05).The area of calvarial sagittal suture osteolysis was (0.262 ± 0.009),(0.130±0.013),(0.307±0.013) and (0.178 ±0.011) mm2 in subgroups A1,A2,B1 and B2 respectively.There was significant difference among the groups.Conclusion Ostoporosis may reduce the particles-induced osteolysis in a rat model.