CAJ | 학술논문

目的探讨Survivin和突变型p53(mp53)在膀胱尿路上皮癌中的表达及临床意义,并分析两者表达的相关性.方法应用免疫组织化学方法检测86例膀胱尿路上皮癌中Survivin和mp53的蛋白表达,每例标本均选用癌中心、癌旁组织,并选正常膀胱黏膜作为对照.结果 Survivin 和mp53在癌组织中的表达明显高于癌旁组织,而且在正常黏膜中均无表达.在膀胱癌不同病理分级中,Survivin阳性表达率分别为:G1组68.00％、G2组85.29％、G3组92.59％,mp53分别为:G1组72.00％、G2组88.24％、G3组96.30％；在不同分期中,Survivin阳性表达率分别为:Tis～T1组为75.00％、T2～T4组为94.12％,mp53分别为:Tis～T1组为80.77％、T2～T4组为94.12％；在淋巴结转移组和非转移组:Survivin阳性表达率为96.00％、77.05％,mp53分别为:96.00％、81.97％,各组间差异均有统计学意义(P＜0.05),而且两者表达呈正相关(r=0.452,P＜0.05).结论 Survivin 和mp53高表达与膀胱尿路上皮癌的恶性程度密切相关,可能在膀胱癌的发生发展中起协同作用.
목적 탐토Survivin화돌변형p53(mp53)재방광뇨로상피암중적표체급림상의의,병분석량자표체적상관성.방법 응용면역조직화학방법검측86례방광뇨로상피암중Survivin화mp53적단백표체,매례표본균선용암중심、암방조직,병선정상방광점막작위대조.결과 Survivin 화mp53재암조직중적표체명현고우암방조직,이차재정상점막중균무표체.재방광암불동병리분급중,Survivin양성표체솔분별위:G1조68.00％、G2조85.29％、G3조92.59％,mp53분별위:G1조72.00％、G2조88.24％、G3조96.30％；재불동분기중,Survivin양성표체솔분별위:Tis～T1조위75.00％、T2～T4조위94.12％,mp53분별위:Tis～T1조위80.77％、T2～T4조위94.12％；재림파결전이조화비전이조:Survivin양성표체솔위96.00％、77.05％,mp53분별위:96.00％、81.97％,각조간차이균유통계학의의(P＜0.05),이차량자표체정정상관(r=0.452,P＜0.05).결론 Survivin 화mp53고표체여방광뇨로상피암적악성정도밀절상관,가능재방광암적발생발전중기협동작용.
Objective To investigate the expression and significance of Survivin and mutant p53(mp53) in bladder urothelial carcinoma,and the relationship between them.Methods The expression of Survivin and mp53 in 86 bladder urothelial carcinoma specimens surgically resected was detected by using immunohistochemistry SP method.For each specimen,the cancerous tissue,paracancerous tissue and the remote normal mucosa were analyzed and compared.Results The immunohistochemisty revealed that the expression of Survivin and mp53 in bladder urothelial carcinoma was significantly higher than that in paracancerous tissue,and no expression was detectable in normal bladder tissues.Among the pathological grades G1,G2 and G3,the positive expression rate of Survivin and mp53 respectively was 68.00％,85.29％,92.59％,and 72.00％,88.24％,96.30％.The positive expression rate of Survivin and mp53in the clinical stages of Tis-T1 was lower than in the stages of T2-T4 (75.00％ vs.94.12％,P ＜ 0.05 ;80.77％ vs.94.12％,P ＜0.05),and that in the group with lymphatic metastasis was higher than in the group with negative lymphatic metastasis (96.00％ vs.77.05％,P ＜ 0.05 ; 96.00％ vs.81.97％,P ＜0.05).There was significant difference among groups (P ＜ 0.05).Moreover,the expression of Survivin was positively correlated with that of mp53 (r =0.452,P ＜ 0.05).Conclusion The overexpression of Survivin and mp53 was closely correlated with the degree of malignancy,and may play synergetic roles in carcinogenesis and progression of bladder urothelial carcinoma.