CAJ | 학술논문

目的探讨前列腺癌(PCa)患者外周血中差异显示编码3基因(DD3) mRNA的表达及其与临床分期及Gleason评分的关系.方法采用逆转录-聚合酶链反应(RT-PCR)法检测63例PCa患者及61例良性前列腺增生(BPH)患者外周血中DD3 mRNA表达,同时分析不同临床分期和病理分级与DD3 mRNA表达的关系.结果 PCa患者外周血中DD3 mRNA表达率为82.5％,BPH组无表达,差异有统计学意义(P＜0.1).不同临床分期的DD3 mRNA表达差异有统计学意义(P＜0.05),不同Gleason评分的DD3 mRNA阳性表达差异有统计学意义(P＜0.05).结论外周血DD3 mRNA表达和前列腺发生发展密切相关.
목적 탐토전렬선암(PCa)환자외주혈중차이현시편마3기인(DD3) mRNA적표체급기여림상분기급Gleason평분적관계.방법 채용역전록-취합매련반응(RT-PCR)법검측63례PCa환자급61례량성전렬선증생(BPH)환자외주혈중DD3 mRNA표체,동시분석불동림상분기화병리분급여DD3 mRNA표체적관계.결과 PCa환자외주혈중DD3 mRNA표체솔위82.5％,BPH조무표체,차이유통계학의의(P＜0.1).불동림상분기적DD3 mRNA표체차이유통계학의의(P＜0.05),불동Gleason평분적DD3 mRNA양성표체차이유통계학의의(P＜0.05).결론 외주혈DD3 mRNA표체화전렬선발생발전밀절상관.
Objective To detect the differential display gene 3 (DD3) mRNA expression in peripheral blood of patients with prostate cancer,and study the relation between DD3 mRNA expression with clinical stage and Gleason score.Methods Peripheral blood DD3 mRNA expression was detected by using reverse transcription-polymerase chain reaction (RT-PCR) in 63 patients with prostate cancer and 61 patients with benign prostate hyperplasia (BPH).The DD3 mRNA expression was compared in groups with different clinical stages and Gleason scores.Results The DD3 mRNA expression rate in prostate cancer group was 82.5％,but no DD3 mRNA expression was detectable in BPH group (P ＜ 0.01).There was significant difference in the DD3 mRNA expression among different stages or Gleason scores.Conclusion The peripheral blood DD3 mRNA expression was correlated significantly with the development and progression of prostate cancer.