中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
2期
399-400
,共2页
血管紧张素Ⅱ%主动脉夹层%模型,动物
血管緊張素Ⅱ%主動脈夾層%模型,動物
혈관긴장소Ⅱ%주동맥협층%모형,동물
Angiotensin Ⅱ%Aortic dissection%Model,animal
目的 应用血管紧张素-Ⅱ(Ang-Ⅱ)腹腔注射建立小鼠主动脉夹层模型.方法 8月龄C57BL/6J小鼠40只,雌雄不拘,随机分为2组.实验组20只,每8h腹腔注射血管紧张素Ⅱ,剂量为每天4.5 mg/kg;对照组20只,每8h腹腔注射去甲肾上腺素,剂量为每天12 mg/kg.分别于注射30 min后应用鼠尾测压器检测并记录小鼠血压(收缩压).精心饲养14 d,中途死亡小鼠直接解剖,取出主动脉.14 d后,存活小鼠颈椎脱臼处死,解剖取出主动脉,大体及光镜观察.结果 实验组小鼠检测血压为(134.5:3.5) mmHg(1 mmHg=0.133 kPa),对照组检测血压为(132.9±2.7) mm Hg,两组血压差异无统计学意义(P＞0.05).实验组中有7只小鼠大体标本观察有主动脉夹层发生,另有12只标本光镜下观察发现内膜明显增厚,中膜变薄,可见断裂,符合主动脉夹层组织病理学表现.对照组所有标本大体及光镜下观察均无明显组织病理学改变.结论 腹腔注射Ang-Ⅱ能建立小鼠主动脉夹层模型.
目的 應用血管緊張素-Ⅱ(Ang-Ⅱ)腹腔註射建立小鼠主動脈夾層模型.方法 8月齡C57BL/6J小鼠40隻,雌雄不拘,隨機分為2組.實驗組20隻,每8h腹腔註射血管緊張素Ⅱ,劑量為每天4.5 mg/kg;對照組20隻,每8h腹腔註射去甲腎上腺素,劑量為每天12 mg/kg.分彆于註射30 min後應用鼠尾測壓器檢測併記錄小鼠血壓(收縮壓).精心飼養14 d,中途死亡小鼠直接解剖,取齣主動脈.14 d後,存活小鼠頸椎脫臼處死,解剖取齣主動脈,大體及光鏡觀察.結果 實驗組小鼠檢測血壓為(134.5:3.5) mmHg(1 mmHg=0.133 kPa),對照組檢測血壓為(132.9±2.7) mm Hg,兩組血壓差異無統計學意義(P＞0.05).實驗組中有7隻小鼠大體標本觀察有主動脈夾層髮生,另有12隻標本光鏡下觀察髮現內膜明顯增厚,中膜變薄,可見斷裂,符閤主動脈夾層組織病理學錶現.對照組所有標本大體及光鏡下觀察均無明顯組織病理學改變.結論 腹腔註射Ang-Ⅱ能建立小鼠主動脈夾層模型.
목적 응용혈관긴장소-Ⅱ(Ang-Ⅱ)복강주사건립소서주동맥협층모형.방법 8월령C57BL/6J소서40지,자웅불구,수궤분위2조.실험조20지,매8h복강주사혈관긴장소Ⅱ,제량위매천4.5 mg/kg;대조조20지,매8h복강주사거갑신상선소,제량위매천12 mg/kg.분별우주사30 min후응용서미측압기검측병기록소서혈압(수축압).정심사양14 d,중도사망소서직접해부,취출주동맥.14 d후,존활소서경추탈구처사,해부취출주동맥,대체급광경관찰.결과 실험조소서검측혈압위(134.5:3.5) mmHg(1 mmHg=0.133 kPa),대조조검측혈압위(132.9±2.7) mm Hg,량조혈압차이무통계학의의(P＞0.05).실험조중유7지소서대체표본관찰유주동맥협층발생,령유12지표본광경하관찰발현내막명현증후,중막변박,가견단렬,부합주동맥협층조직병이학표현.대조조소유표본대체급광경하관찰균무명현조직병이학개변.결론 복강주사Ang-Ⅱ능건립소서주동맥협층모형.
Objective To establish the mouse aorta dissection model through intraperitoneal injection of angiotensin Ⅱ.Methods Forty 8-month-old C.57BL/6J mice without gender limitaiton were randomly divided into two groups (n =20 each).The animals in experimental group received intraperitoneal injection of angiotensin Ⅱ every 8 h,4.5 mg/kg every day,and those in control group received intraperitoneal injection of noradrenaline every 8 h,12 mg/kg every day.Thirty min after injection,blood pressure was measured using tail cuff blood pressure device (systolic pressure).All mice were fed carefully for 14days.If mice died during this period,they were anatomized directly in order to take aorta tissue.Fourteen days later,the rest mice were killed and aorta walls were taken to observe the structural changes pathologically.Results The blood pressure in experimental group was (134.5 ± 3.5) mm Hg (1 mm Hg =0.133 kPa),and (132.9 ± 2.7) mmHg in control group respectively (P ＞ 0.05).In experimental group,the tuniea media of arteriae aorta walls of 12 mice were thinner and some of them were broken.Also,obvious aortic dissection was found in 7 mice of experimental,but no significant pathological changes were found in control group.Conclusion The mouse aorta dissection model can be established effectivly through intraperitoneal injection of angiotensin Ⅱ.
