CAJ | 학술논문

目的建立大鼠高肺血流量肺动脉高压模型.方法采取左颈总动脉-颈外静脉分流、左肺动脉结扎以及左颈总动脉-颈外静脉分流联合左肺动脉结扎3种手术方式建立大鼠高肺血流量模型,观察术后8周肺动脉平均压(mPAP)、右心室肥厚指数(RV/LV+S)、肺血管管壁厚度占血管外径的百分比(WT％)和管壁面积占血管总面积的百分比(WA％)；以及左颈总动静脉分流联合左肺动脉结扎术后4、8、12周上述指标的变化.结果与对照组比较,左颈总动脉-颈外静脉分流联合左肺动脉结扎在术后8、12周产生明显肺动脉高压,mPAP及RV/LV +S逐渐增加,且差异有统计学意义(P＜0.01),WT％和WA％差异也均有统计学意义(P＜0.01).结论左颈总动脉-颈外静脉分流联合左肺动脉结扎可建立高肺血流量肺动脉高压模型,且操作简单.
목적 건립대서고폐혈류량폐동맥고압모형.방법 채취좌경총동맥-경외정맥분류、좌폐동맥결찰이급좌경총동맥-경외정맥분류연합좌폐동맥결찰3충수술방식건립대서고폐혈류량모형,관찰술후8주폐동맥평균압(mPAP)、우심실비후지수(RV/LV+S)、폐혈관관벽후도점혈관외경적백분비(WT％)화관벽면적점혈관총면적적백분비(WA％)；이급좌경총동정맥분류연합좌폐동맥결찰술후4、8、12주상술지표적변화.결과 여대조조비교,좌경총동맥-경외정맥분류연합좌폐동맥결찰재술후8、12주산생명현폐동맥고압,mPAP급RV/LV +S축점증가,차차이유통계학의의(P＜0.01),WT％화WA％차이야균유통계학의의(P＜0.01).결론 좌경총동맥-경외정맥분류연합좌폐동맥결찰가건립고폐혈류량폐동맥고압모형,차조작간단.
Objective To establish a high-flow type of rat pulmonary hypertension model which can recapitulate clinical characteristics of pulmonary arterial hypertension in congenital heart disease with systemic-to-pulmonary shunts.Methods The animal model established by either left common carotid artery-external jugular vein shunts,or ligation of the left pulmonary artery,or combination of the two methods.Changes in mean pulmonary artery pressure (mPAP),right ventricular hypertrophy index of right ventricle to left ventricle plus interventricular septum (RV/LV + S),the ratio of vascular wall thickness to vascular external diameter (WT％) and the ratio of vascular wall area to total vascular area (WA％) were observed to compare different surgical approaches during postoperative 8 week.The changes of above indexes were also observed following combination of the two methods at 4,8 and 12 week postoperation.Results As compared with control group,the mPAP and RV/LV + S in combination of shunt and ligation of the left pulmonary artery group were significantly increased at 8th and 12th week (P ＜ 0.01) ; Compared with control group,the WT％ and WA％ also elevated obviously (P ＜ 0.01).Conclusion Combination of shunt and ligation of the left pulmonary artery is an easy and reliable approach to create high flow-induced pulmonary arterial hypertension in rats,and can be used to study the pathogenesis of pulmonary arterial hypertension caused by congenital heart diseases.