CAJ | 학술논문

目的观察神经节苷脂(GMI)对脊髓损伤后内质网应激介导凋亡的影响.方法 90只SD大鼠按随机数字表法分为假手术组、等渗盐水组和GM1组,每组30只.等渗盐水组和GM1组采用Allen法建立T10脊髓损伤动物模型,分别于伤后3h、1、3、7d时(每个时间点6只)应用改良Tarlov评分法评价其神经功能；应用Hochest33658染色检测神经细胞凋亡；荧光显微镜下观察内源性半胱氨酰天冬氨酸特异性蛋白酶(Caspase)-12、CHOP的表达.Western blot法检测内质网应激相关蛋白Caspase-12、CHOP的表达.进一步细胞水平STS(5μmol/L)诱导人神经母细胞瘤细胞(SH-SY5Y)细胞凋亡12h后加入GM1(0、20、50 mmol/L)孵育48 h,Western blot检测Caspase-12及CHOP表达；碘化丙锭(PI)/膜联蛋白V(Annexin V)-异硫氰酸荧光素(FITC)检测细胞凋亡率.结果假手术组大鼠均未见明显瘫痪,等渗盐水组及GM1组出现不同程度的下肢功能障碍,且各时间点的Tarlov评分均低于假手术组(P＜0.01).与假手术组比较,等渗盐水组Caspase-12及CHOP表达水平分别为0.24±0.02及0.63±0.10,明显上调(P＜0.05),细胞凋亡率明显上调(P＜0.05)；与等渗盐水组比较GM1组Caspase-12及CHOP表达水平分别为0.13±0.01及0.43 ±0.07,均下调(P＜0.05),细胞凋亡率下调(P＜0.05)；GM1孵育后STS诱导的SH-SY5Y细胞凋亡率下降7.4±6.9(P ＜0.05),Caspase-12及CHOP表达水平下调(P＜0.05).结论 GM1通过下调Caspase-12及CHOP表达抑制了大鼠脊髓损伤后内质网应激介导的神经元凋亡.
목적 관찰신경절감지(GMI)대척수손상후내질망응격개도조망적영향.방법 90지SD대서안수궤수자표법분위가수술조、등삼염수조화GM1조,매조30지.등삼염수조화GM1조채용Allen법건립T10척수손상동물모형,분별우상후3h、1、3、7d시(매개시간점6지)응용개량Tarlov평분법평개기신경공능；응용Hochest33658염색검측신경세포조망；형광현미경하관찰내원성반광안선천동안산특이성단백매(Caspase)-12、CHOP적표체.Western blot법검측내질망응격상관단백Caspase-12、CHOP적표체.진일보세포수평STS(5μmol/L)유도인신경모세포류세포(SH-SY5Y)세포조망12h후가입GM1(0、20、50 mmol/L)부육48 h,Western blot검측Caspase-12급CHOP표체；전화병정(PI)/막련단백V(Annexin V)-이류청산형광소(FITC)검측세포조망솔.결과 가수술조대서균미견명현탄탄,등삼염수조급GM1조출현불동정도적하지공능장애,차각시간점적Tarlov평분균저우가수술조(P＜0.01).여가수술조비교,등삼염수조Caspase-12급CHOP표체수평분별위0.24±0.02급0.63±0.10,명현상조(P＜0.05),세포조망솔명현상조(P＜0.05)；여등삼염수조비교GM1조Caspase-12급CHOP표체수평분별위0.13±0.01급0.43 ±0.07,균하조(P＜0.05),세포조망솔하조(P＜0.05)；GM1부육후STS유도적SH-SY5Y세포조망솔하강7.4±6.9(P ＜0.05),Caspase-12급CHOP표체수평하조(P＜0.05).결론 GM1통과하조Caspase-12급CHOP표체억제료대서척수손상후내질망응격개도적신경원조망.
Objective To investigate the effects of monosialotetrahexosy 1 ganglioside (GM1) on endoplasmic reticulum stress-mediated apoptosis following spinal cord injury (SCI).Methods Ninety SD rats were randomly and equally assigned to the sham-operation group,the isotonic saline group and the GM1 group.Rat T10 SCI model was established by using Allen method in both the isotonic saline group and the GM1 group.The nerve function of spinal cord was evaluated by modified Tarlov score after the animals were scarificed at 3rd h,24th h,3rd day,and 7th day respectively after injury..The apoptosis was determined with Hochest 33658 staining.The expression of endogenous cysteinyl aspartate-specific protease (Caspase)-12 and CHOP protein was observed by fluorescence microscopy.The cell total protein was extracted for detection of the endoplasmic reticulum stress related Caspase-12 and CHOP protein expression by using Western blotting.Further at the cellular level,the apoptosis of human neuroblastoma cells (SH-SY5Y) was induced by STS (5 μmol/L),and 12 h later,the cells were added with GM1 (0,20 and 50 mmol/L),and incubated for 48 h.The expression of Caspase-12 and CHOP proteins was detected by using Western blotting,and the apoptosis rate was determined with propidium iodide (PI)/Annexin V-FITC staining by flow cytometry analysis.Results Different levels of paralysis were evidenced in the isotonic saline group and the GM1 group,but not in the sham-operation group,with the Tarlov score in the former much lower than that in the latter at each time point (P ＜ 0.01).As compared with the sham-operation group,the levels of Caspase-12 and CHOP protein expression (0.24 ±0.02 and 0.63 ±0.10) were increased (P ＜ 0.05),and the apotosis rate was reduced (P ＜ 0.05) in the isotonic saline group.As compared with the isotonic saline group,the levels of Caspase-12 and CHOP protein expression (0.13 ± 0.01 and 0.43 ± 0.07) was reduced (P ＜ 0.05),and the apoptosis rate was declined (P ＜ 0.05) in the GM1 group.After addion of GM1,the cell apoptosis rate was cut down (7.4 ± 6.9) (P ＜ 0.05),and the levels of Caspase-12 and CHOP protein expression were reduced (P ＜ 0.05).Conclusion GM1 can inhibit endoplasmic reticulum stress-mediated apoptosis following SCI by reducing the levels of Caspase-12 and CHOP protein expression.