中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
7期
1377-1379
,共3页
邓晔坤%顾军%周晓中%宋祥胜%刘洪鹏%佘昶%董启榕
鄧曄坤%顧軍%週曉中%宋祥勝%劉洪鵬%佘昶%董啟榕
산엽곤%고군%주효중%송상성%류홍붕%사창%동계용
低剂量照射%血管内皮生长因子%骨髓间充质干细胞
低劑量照射%血管內皮生長因子%骨髓間充質榦細胞
저제량조사%혈관내피생장인자%골수간충질간세포
Low-dose X-irradiation%Vascular endothelial growth factor%Bone marrow mesenchymal stem cells
目的 观察低剂量照射(LDI)对血管内皮生长因子(VEGF)和骨髓间充质干细胞(BMSCs)的影响,探讨其促进骨痂矿化的机制.方法 LDI 干预下,分别分析血清和骨痂内VEGF表达的变化,同时检测原代BMSC的成骨分化情况.结果 实验组血清VEGF含量在术后第3周达(315.00±21.33) ng/L,明显高于对照组(175.05±18.17) ng/L(P <0.05).术后第2和第3周,实验组骨痂内VEGF mRNA的表达和新生血管数量明显高于对照组(P <0.05);10 cGy的LDI可明显刺激BMSCs的增殖(1.697±0.048,照射后24h)(P <0.05) 10cGy实验组矿化结节数目[(24.0±1.6)个]较对照组明显增多(P<0.05),且骨保护素基因(OPG)、Ⅰ型胶原(COL-1)、骨钙素基因(BGP)的表达也明显高于对照组(P<0.05).结论 LDI通过上调VEGF的表达和促进BMSCs的动员,促进了骨痂的矿化.
目的 觀察低劑量照射(LDI)對血管內皮生長因子(VEGF)和骨髓間充質榦細胞(BMSCs)的影響,探討其促進骨痂礦化的機製.方法 LDI 榦預下,分彆分析血清和骨痂內VEGF錶達的變化,同時檢測原代BMSC的成骨分化情況.結果 實驗組血清VEGF含量在術後第3週達(315.00±21.33) ng/L,明顯高于對照組(175.05±18.17) ng/L(P <0.05).術後第2和第3週,實驗組骨痂內VEGF mRNA的錶達和新生血管數量明顯高于對照組(P <0.05);10 cGy的LDI可明顯刺激BMSCs的增殖(1.697±0.048,照射後24h)(P <0.05) 10cGy實驗組礦化結節數目[(24.0±1.6)箇]較對照組明顯增多(P<0.05),且骨保護素基因(OPG)、Ⅰ型膠原(COL-1)、骨鈣素基因(BGP)的錶達也明顯高于對照組(P<0.05).結論 LDI通過上調VEGF的錶達和促進BMSCs的動員,促進瞭骨痂的礦化.
목적 관찰저제량조사(LDI)대혈관내피생장인자(VEGF)화골수간충질간세포(BMSCs)적영향,탐토기촉진골가광화적궤제.방법 LDI 간예하,분별분석혈청화골가내VEGF표체적변화,동시검측원대BMSC적성골분화정황.결과 실험조혈청VEGF함량재술후제3주체(315.00±21.33) ng/L,명현고우대조조(175.05±18.17) ng/L(P <0.05).술후제2화제3주,실험조골가내VEGF mRNA적표체화신생혈관수량명현고우대조조(P <0.05);10 cGy적LDI가명현자격BMSCs적증식(1.697±0.048,조사후24h)(P <0.05) 10cGy실험조광화결절수목[(24.0±1.6)개]교대조조명현증다(P<0.05),차골보호소기인(OPG)、Ⅰ형효원(COL-1)、골개소기인(BGP)적표체야명현고우대조조(P<0.05).결론 LDI통과상조VEGF적표체화촉진BMSCs적동원,촉진료골가적광화.
Objective To explore the inechanism of low-dose X-irradiation (LDI) promoting callus mineralization through vascular endothelial growth factor (VEGF) and hone marrow mesenchymal stem cells (BMSCs).Methods The expression of VEGF in serum and callus samples was quantified and the osteogenic differentiation of BMSCs was detected after exposure.Results MicroCT based angiography visually revealed that the number of neovascularization was conspicuously increased since 2 weeks in the LD1 group [(315.00 ±21.33) ng/L] as compared with that in sham group [(175.05 ± 18.17) ng/L] (P <0.05).The serum VEGF in the LDI group was higher than in control group (P < 0.05) at 3rd week.Elevated mRNA expression of VEGF was observed,and reached the peak at 2nd and 3rd week respectively in the LDI group as compared with control group (P < 0.05).The cell counting kit-8 (CCK-8) assay showed that the proliferation of BMSCs was accelerated significantly in vitro in the 10 cGy irradiation group (1.697 ±0.048,24 h after exposure) (P < 0.05).Mineralized nodule number in the LDI group [(24.0 ± 1.6)] was greater thau in control group,especially in the 10 cGy group (P <0.05).RT-PCR showed that the expression of Osteoprotegerin (OPG),collagenase-1 (COL-1),and osteocalcin (BGP) genes was increased in the LDI groups,especially in the 10 cGy group (P < 0.05).Conclusion The results indicate that LDI promotes callus mineralization through up-regulating VEGF and promoting the mobilization of BMSCs.