CAJ | 학술논문

目的建立辐射诱发裸小鼠骨髓功能毁损与示踪骨髓移植重建的动物模型.方法分别用4、6、8、10Gy X线辐照裸小鼠,检测辐照后第1、3、5、7、14、21天小鼠血象变化,确定适合裸小鼠骨髓功能毁损的辐照剂量.用确定剂量辐照后1周进行尾静脉移植表达绿色荧光蛋白(GFP)裸小鼠的骨髓细胞(2×10 7个/只),通过实时检测其在外周血中的分布,并比较移植前后外周血白细胞和骨髓细胞,以评价骨髓功能.结果 8 Gy辐照后裸小鼠外周血白细胞为(0.1375 ±0.0510)×109/L,骨髓细胞是辐照前的(31.73±2.56)％.辐照后1周骨髓移植鼠外周血白细胞在28 d达到(12.46 ±0.68)× 109/L,恢复到辐照前水平[(12.89±1.04)×109/L,P＞0.05].外周血中GFP+细胞占(63.0±0.6)％.结论 8 Gy是建立裸小鼠骨髓功能重度毁损重建较为适合的辐照剂量；尾静脉注射GFP+供体骨髓细胞,能有效示踪受体骨髓功能重建主要依赖于供体的骨髓细胞.
목적 건립복사유발라소서골수공능훼손여시종골수이식중건적동물모형.방법 분별용4、6、8、10Gy X선복조라소서,검측복조후제1、3、5、7、14、21천소서혈상변화,학정괄합라소서골수공능훼손적복조제량.용학정제량복조후1주진행미정맥이식표체록색형광단백(GFP)라소서적골수세포(2×10 7개/지),통과실시검측기재외주혈중적분포,병비교이식전후외주혈백세포화골수세포,이평개골수공능.결과 8 Gy복조후라소서외주혈백세포위(0.1375 ±0.0510)×109/L,골수세포시복조전적(31.73±2.56)％.복조후1주골수이식서외주혈백세포재28 d체도(12.46 ±0.68)× 109/L,회복도복조전수평[(12.89±1.04)×109/L,P＞0.05].외주혈중GFP+세포점(63.0±0.6)％.결론 8 Gy시건립라소서골수공능중도훼손중건교위괄합적복조제량；미정맥주사GFP+공체골수세포,능유효시종수체골수공능중건주요의뢰우공체적골수세포.
Objective To establish the green fluorescent protein (GFP) + bone marrow reconstituted nude mice model.Methods Different doses (4,6,8,10 Gy) X-ray were used to irradiate the nude mice.The hemogram was detected separately 1,3,5,7,14 and 21 days after irradiation in order to confirm the proper irradiation dose for bone marrow damage.One-week after irradiation with such dose,allogeneic transplantation with GFP + bone marrow cells (BMCs) (2 × 107 cells/mice) were executed through the caudal vein.The proportion of GFP + donor cells in peripheral blood was tested.White blood cells (WBCs) of peripheral blood and BMCs were compared before and after transplantation.Results After irradiation with 8 Gy,the number of peripheral blood WBCs was (0.1375 ±0.0510) × 109/L,and BMCs were [(31.73 ±2.56)％] of pre-irradiation nude mice.At 28th day after BMCs transplantion,the number of peripheral blood WBCs was (12.46 ± 0.68) × 109/L and recovered to the level pre-irradiation (12.89 ± 1.04) × 109/L (P ＞ 0.05).The proportion of donor GFP + cells was (63.0 ± 0.6) ％ in peripheral blood.Conclusion Eight Gy is the suitable dose for establishment of the bone marrow damage reconstituted nude mice model.Tail vein injection of GFP + BMCs effectively indicated the reconstitution of bone marrow function,which mainly depends on the donor BMCs.