CAJ | 학술논문

目的在肝脏局部流出道梗阻的基础上建立肝癌实验模型,观察肝脏出肝血流不畅时对肝癌细胞肝内播散的影响.方法 16只雌性Buffalo大鼠分为两组,A组为对照组(SO),B组临时阻断肝脏左叶出肝静脉(OHV),并监测术中门静脉血流的变化.同时在两组大鼠肝内注入荧光标记的肝癌细胞.术后取下肝脏标本在荧光显微镜下观察两组模型肝内的肿瘤定植,常规观察流出道不畅后的肝脏病理变化.结果当大鼠肝脏左叶出肝静脉阻断后,入肝方向的门静脉血流由正常时的(11.00±3.13) ml/min下降为(8.42±1.99) ml/min.术后常规病理发现肝窦内大量淤血.荧光显微镜下观察,OHV组较SO组,肝内含有荧光标记的肿瘤明显增多(P＜0.05).结论当肝脏流出道不畅造成肝内血流紊乱时,侵入脉管系统的肝癌细胞容易滞留于肝内,形成肝内转移灶.
목적 재간장국부류출도경조적기출상건립간암실험모형,관찰간장출간혈류불창시대간암세포간내파산적영향.방법 16지자성Buffalo대서분위량조,A조위대조조(SO),B조림시조단간장좌협출간정맥(OHV),병감측술중문정맥혈류적변화.동시재량조대서간내주입형광표기적간암세포.술후취하간장표본재형광현미경하관찰량조모형간내적종류정식,상규관찰류출도불창후적간장병리변화.결과 당대서간장좌협출간정맥조단후,입간방향적문정맥혈류유정상시적(11.00±3.13) ml/min하강위(8.42±1.99) ml/min.술후상규병리발현간두내대량어혈.형광현미경하관찰,OHV조교SO조,간내함유형광표기적종류명현증다(P＜0.05).결론 당간장류출도불창조성간내혈류문란시,침입맥관계통적간암세포용역체류우간내,형성간내전이조.
Objective To explore the effects of blood disturbance after hepatic venous occlusion procedures on intrahepatic metastasis of liver cancer cells in rats.Methods Sixteen female buffalo rats were randomly divided into occlusion of the hepatic vein (OHV) group and sham operation (SO) group.The portal flow was observed while the hepatic vein was clamped in the OHV group.After hepatic vein was clamped in the OHV group,the liver cancer cells labeled with the fluorescent dye were injected into the portal vein and the same with SO group.The animals were sacrificed at 2 h after the operation for the samples.Dil-labeled liver cancer cells in the liver were then observed under fluorescent microscopy.Histological analysis was taken to adjust the influence of hepatic outflow occlusion on liver environment.Results The OHV group showed an obvious decrease in portal flow,from (11.00 ±3.13) to (8.42 ± 1.99) ml/min after vein clamping.Pathological analysis also found numerous congestions around blood vessels and sinusoid and more Dil-labeled cancer cells in the livers of OHV group.Conclusion Occlusion of hepatic outflow may promote intrahepatic metastasis of cancer cells by enhancing the opportunity of stasis in the liver.