CAJ | 학술논문

目的探讨胆管癌细胞株QBC939与正常胆管上皮细胞株HIBEC中微小RNA(miRNA)分子表达谱的差异.方法选取胆管癌细胞株QBC939与正常胆管上皮细胞株HIBEC各6个样本分别作为实验组和对照组,采用miRNA芯片筛选两者之间差异表达的miRNA,样品间表达变化标准以上/下调2倍作为域值范围,并用实时定量聚合酶链反应(Real-time PCR)验证miRNA芯片结果的可靠性.结果在胆管癌细胞株中,上调2倍差异表达miRNA分子72个,上调超过8倍差异表达miRNA 6个；下调2倍差异表达miRNA分子61个,下调超过8倍miRNA分子5个(P＜0.05).结论胆管癌细胞株QBC939与正常胆管上皮细胞株HIBEC之间存在差异表达miRNAs分子,它们可能与胆管癌的发生和转移有关.
목적 탐토담관암세포주QBC939여정상담관상피세포주HIBEC중미소RNA(miRNA)분자표체보적차이.방법 선취담관암세포주QBC939여정상담관상피세포주HIBEC각6개양본분별작위실험조화대조조,채용miRNA심편사선량자지간차이표체적miRNA,양품간표체변화표준이상/하조2배작위역치범위,병용실시정량취합매련반응(Real-time PCR)험증miRNA심편결과적가고성.결과 재담관암세포주중,상조2배차이표체miRNA분자72개,상조초과8배차이표체miRNA 6개；하조2배차이표체miRNA분자61개,하조초과8배miRNA분자5개(P＜0.05).결론 담관암세포주QBC939여정상담관상피세포주HIBEC지간존재차이표체miRNAs분자,타문가능여담관암적발생화전이유관.
Objective To explore microRNA differential expression profile between cholangiocarcinoma cell line QBC939 and normal bile duct cell line HIBEC.Methods Six samples from QBC939 cell line and another 6 samples from normal bile duct cell line HIBEC were chosen as the experimental group and the control group,respectively.MicroRNA profiles in these samples were analyzed by microarray.The threshold value used to screen up-and down-regulated microRNAs was fold chang ＞ 2.Real-time quantitative polymerase chain reaction (Real-time PCR) was used to validate the reliability of microarray.Results Six microRNAs were up-regulated in the experimental group and were more than 8 times higher than in the control group.Five microRNAs were down-regulated and were more than 8 times higher than in the control group.Conclusion These microRNAs with differential expression might be related with the formation and metastasis of cholangiocarcinoma.