中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
7期
1507-1510
,共4页
周崇治%韩杨%凃威伟%王晓亮%裘国强%彭志海
週崇治%韓楊%凃威偉%王曉亮%裘國彊%彭誌海
주숭치%한양%도위위%왕효량%구국강%팽지해
同源盒基因家族%胃癌%HOXA10%HOXA13
同源盒基因傢族%胃癌%HOXA10%HOXA13
동원합기인가족%위암%HOXA10%HOXA13
Homeobox gene family%Gastric cancer%HOXA10%HOXA13
目的 观察在胃癌中同源盒(HOX)基因家族表达,探讨两者的相关性.方法 采用Affymetrix U133 plus 2.0人类全基因组表达谱芯片分析在胃癌及其对应正常黏膜中HOX基因家族的差异表达,并行统计学分析.结果 HOXA基因家族中HOXA10、HOXA13在胃癌组织中表达明显升高,高表达率均为91.7％ (11/12),其TvsN Signal Log Ratio值分别为0.4、5.4、4.2、6.1、7.1、5.2、3.4、1.9、1.6、3.0、3.5、3.3和1、4.8、0.3、6.9、4.9、3.5、4.2、4.3、5.4、4.1、2.2、2.8.同时HOX基因家族中还有多个基因表达上调,其中HOXA家族中HOXA1、HOXA4在胃癌表达升高,表达率均为66.7％ (8/12)；HOXB家族中HOXB7在胃癌中表达升高,表达率为75.0％ (9/12)；HOXC家族中HOXC10也表达升高,表达率为58.3％ (7/12)；而HOXC5和HOXC8则在肿瘤中呈现低表达,表达率分别为66.7％ (8/12)和58.3％ (7/12).结论 HOX家族中多个基因在胃癌中差异表达,提示该基因家族与胃癌发生发展密切相关.
目的 觀察在胃癌中同源盒(HOX)基因傢族錶達,探討兩者的相關性.方法 採用Affymetrix U133 plus 2.0人類全基因組錶達譜芯片分析在胃癌及其對應正常黏膜中HOX基因傢族的差異錶達,併行統計學分析.結果 HOXA基因傢族中HOXA10、HOXA13在胃癌組織中錶達明顯升高,高錶達率均為91.7％ (11/12),其TvsN Signal Log Ratio值分彆為0.4、5.4、4.2、6.1、7.1、5.2、3.4、1.9、1.6、3.0、3.5、3.3和1、4.8、0.3、6.9、4.9、3.5、4.2、4.3、5.4、4.1、2.2、2.8.同時HOX基因傢族中還有多箇基因錶達上調,其中HOXA傢族中HOXA1、HOXA4在胃癌錶達升高,錶達率均為66.7％ (8/12)；HOXB傢族中HOXB7在胃癌中錶達升高,錶達率為75.0％ (9/12)；HOXC傢族中HOXC10也錶達升高,錶達率為58.3％ (7/12)；而HOXC5和HOXC8則在腫瘤中呈現低錶達,錶達率分彆為66.7％ (8/12)和58.3％ (7/12).結論 HOX傢族中多箇基因在胃癌中差異錶達,提示該基因傢族與胃癌髮生髮展密切相關.
목적 관찰재위암중동원합(HOX)기인가족표체,탐토량자적상관성.방법 채용Affymetrix U133 plus 2.0인류전기인조표체보심편분석재위암급기대응정상점막중HOX기인가족적차이표체,병행통계학분석.결과 HOXA기인가족중HOXA10、HOXA13재위암조직중표체명현승고,고표체솔균위91.7％ (11/12),기TvsN Signal Log Ratio치분별위0.4、5.4、4.2、6.1、7.1、5.2、3.4、1.9、1.6、3.0、3.5、3.3화1、4.8、0.3、6.9、4.9、3.5、4.2、4.3、5.4、4.1、2.2、2.8.동시HOX기인가족중환유다개기인표체상조,기중HOXA가족중HOXA1、HOXA4재위암표체승고,표체솔균위66.7％ (8/12)；HOXB가족중HOXB7재위암중표체승고,표체솔위75.0％ (9/12)；HOXC가족중HOXC10야표체승고,표체솔위58.3％ (7/12)；이HOXC5화HOXC8칙재종류중정현저표체,표체솔분별위66.7％ (8/12)화58.3％ (7/12).결론 HOX가족중다개기인재위암중차이표체,제시해기인가족여위암발생발전밀절상관.
Objective To screen the expression of homeobox (HOX) gene family in gastric cancers and explore the relationships between them.Methods Differential expression of HOX genes in gastric cancers and their corresponding normal mucosa was detected by using whole human genome microarray (Affymetrix GeneChip U133 Arrays plus2.0) and then statistically analyzed.Results HOXA10 and HOXA13 were more significantly highly expressed in the cancerous tissues than in their normal mucosa,and their expression rate was 91.7％ (11/12).Their detailed TvsN Signal Ratio was 0.4,5.4,4.2,6.1,7.1,5.2,3.4,1.9,1.6,3.0,3.5,3.3 and 1.0,4.8,0.3,6.9,4.9,3.5,4.2,4.3,5.4,4.1,2.2,2.8 respectively.Meanwhile,there were several other differentially expressed genes in HOX gene family.HOXA1,HOXA4,HOXB7 and HOXC10 had a higher expression in tumors than in their normal tissues,and their expression rate was 66.7％ (8/12),66.7％ (8/12),75.0％ (9/12),58.3％ (7/12) respectively.However,HOXC5 and HOXC8 were down-regulated in tumors,and their expression rate was 66.7％ (8/12) and 58.3％ (7/12).Conclusion Several differentially expressed HOX genes were found in gastric cancers and might be closely involved in the process of the gastric carcinogenesis.
