中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
7期
1514-1516
,共3页
王长庭%邱成志%许天文%苏志明
王長庭%邱成誌%許天文%囌誌明
왕장정%구성지%허천문%소지명
结直肠癌%性别决定基因相关HMG盒基因4%β-连环蛋白%免疫组织化学
結直腸癌%性彆決定基因相關HMG盒基因4%β-連環蛋白%免疫組織化學
결직장암%성별결정기인상관HMG합기인4%β-련배단백%면역조직화학
Colorectal cancer%Sex determining genes related to HMG box gene 4%β-catenin%Immunohistochemistry
目的 观察结直肠癌组织中性别决定基因相关HMG盒基因4(Sox4)、β-连环蛋白(β-catenin)的表达,探讨Sox4、β-catenin在结直肠癌组织中表达的意义及其相关性.方法 采用免疫组织化学染色方法检测80例结直肠癌组织及20例其邻近正常结直肠组织中Sox4、β-catenin的表达,分析其在结直肠癌组织中的表达与临床病理参数间的关系.结果 Sox4在正常结直肠组织黏膜中未见Sox4表达,在结直肠癌组织中高表达,阳性表达率为68.75％(55/80),Sox4在结直肠癌组织中的表达与患者年龄、性别、肿瘤部位、浸润深度、分化程度无明显相关(P＞0.05),Sox4在伴有淋巴结转移、Ⅲ～Ⅳ期的结直肠癌组织中的表达率分别明显高于无淋巴结转移、Ⅰ～Ⅱ期的表达率(51.2％,P ＜0.05).β-catenin在正常结直肠组织黏膜中主要表达于细胞膜,在结直肠癌组织中异位表达,异位表达阳性率为63.75％ (51/80)；β-catenin在结直肠癌组织中异位表达与患者年龄、性别、肿瘤部位、浸润深度无明显相关(P＞0.05).β-catenin在伴有淋巴结转移、中高分化、Ⅲ～Ⅳ期的结直肠癌组织中的表达率分别明显高于无淋巴结转移、低分化、Ⅰ～Ⅱ期(P＜0.05).结直肠癌组织中Sox4表达与β-catenin表达两者呈明显正相关(r=0.501,P ＜0.01).结论 Sox4和β-catenin在结直肠癌组织中异常表达,参与结直肠癌的发生、发展,两者之间密切相关.
目的 觀察結直腸癌組織中性彆決定基因相關HMG盒基因4(Sox4)、β-連環蛋白(β-catenin)的錶達,探討Sox4、β-catenin在結直腸癌組織中錶達的意義及其相關性.方法 採用免疫組織化學染色方法檢測80例結直腸癌組織及20例其鄰近正常結直腸組織中Sox4、β-catenin的錶達,分析其在結直腸癌組織中的錶達與臨床病理參數間的關繫.結果 Sox4在正常結直腸組織黏膜中未見Sox4錶達,在結直腸癌組織中高錶達,暘性錶達率為68.75％(55/80),Sox4在結直腸癌組織中的錶達與患者年齡、性彆、腫瘤部位、浸潤深度、分化程度無明顯相關(P＞0.05),Sox4在伴有淋巴結轉移、Ⅲ～Ⅳ期的結直腸癌組織中的錶達率分彆明顯高于無淋巴結轉移、Ⅰ～Ⅱ期的錶達率(51.2％,P ＜0.05).β-catenin在正常結直腸組織黏膜中主要錶達于細胞膜,在結直腸癌組織中異位錶達,異位錶達暘性率為63.75％ (51/80)；β-catenin在結直腸癌組織中異位錶達與患者年齡、性彆、腫瘤部位、浸潤深度無明顯相關(P＞0.05).β-catenin在伴有淋巴結轉移、中高分化、Ⅲ～Ⅳ期的結直腸癌組織中的錶達率分彆明顯高于無淋巴結轉移、低分化、Ⅰ～Ⅱ期(P＜0.05).結直腸癌組織中Sox4錶達與β-catenin錶達兩者呈明顯正相關(r=0.501,P ＜0.01).結論 Sox4和β-catenin在結直腸癌組織中異常錶達,參與結直腸癌的髮生、髮展,兩者之間密切相關.
목적 관찰결직장암조직중성별결정기인상관HMG합기인4(Sox4)、β-련배단백(β-catenin)적표체,탐토Sox4、β-catenin재결직장암조직중표체적의의급기상관성.방법 채용면역조직화학염색방법검측80례결직장암조직급20례기린근정상결직장조직중Sox4、β-catenin적표체,분석기재결직장암조직중적표체여림상병리삼수간적관계.결과 Sox4재정상결직장조직점막중미견Sox4표체,재결직장암조직중고표체,양성표체솔위68.75％(55/80),Sox4재결직장암조직중적표체여환자년령、성별、종류부위、침윤심도、분화정도무명현상관(P＞0.05),Sox4재반유림파결전이、Ⅲ～Ⅳ기적결직장암조직중적표체솔분별명현고우무림파결전이、Ⅰ～Ⅱ기적표체솔(51.2％,P ＜0.05).β-catenin재정상결직장조직점막중주요표체우세포막,재결직장암조직중이위표체,이위표체양성솔위63.75％ (51/80)；β-catenin재결직장암조직중이위표체여환자년령、성별、종류부위、침윤심도무명현상관(P＞0.05).β-catenin재반유림파결전이、중고분화、Ⅲ～Ⅳ기적결직장암조직중적표체솔분별명현고우무림파결전이、저분화、Ⅰ～Ⅱ기(P＜0.05).결직장암조직중Sox4표체여β-catenin표체량자정명현정상관(r=0.501,P ＜0.01).결론 Sox4화β-catenin재결직장암조직중이상표체,삼여결직장암적발생、발전,량자지간밀절상관.
Objective To study the expression and significance of sex determining genes related to HMG box gene 4 (Sox4) and β-catenin in colorectal cancer and their correlation.Methods Immunohistochemistry was used to detect the expression of Sox4 and β-catenin in 80 cases of colorectal cancer tissues and 20 cases of nearby normal colorectal tissues,and the relationship between the expression of Sox4 and β-catenin with the clinicopathological parameters was analyzed.Results The Sox4 was mainly expressed in the cell nucleus and the positive rate of Sox4 expresssion in colorectal cancer was 68.75％ (55/80),but no expression of Sox4 was detected in normal colorectal mucosa.The expression of Sox4 in colorectal cancer was not related to the age,gender,tumor site,the tumor invasion depth and the degree of differentiation (P ＞ 0.05).The expression of Sox4 was positively associated with lymph node metastasis and clinical stage in colorectal cancer (P ＜ 0.05).The β-catenin was mainly expressed in the cell membrane in the normal colorectal mucosa,but it was mainly expressed in the cell plasma in colorectal cancer,and the positive rate of the β-catenin ectopic expression in colorectal cancer was 63.75％ (51/80).The ectopic expression of β-catenin in colorectal cancer was not related to the age,gender,tumor site,and the tumor invasion depth (P ＞ 0.05).The expression of β-catenin was positively associated with lymph node metastasis,histological differentiation,and clinical stage in colorectal cancer (P ＜ 0.05).There was a positive correlation between the expression of Sox4 and β-catenin (r =0.501,P ＜ 0.01).Conclusion Sox4 and β-catenin were abnormally expressed in colorectal cancer,and they may play important roles in the tumorigenesis and progression of colorectal cancer and there is a close relationships between them.
