CAJ | 학술논문

目的创建1种新的制备大鼠脊髓损伤模型的手术方法.方法将实验大鼠分为4组:传统蚕食法开窗假手术组(A组)、胸椎揭盖开窗假手术组(B组)、传统蚕食法开窗脊髓打击组(C组)、胸椎揭盖开窗脊髓打击组(D组),分别记录手术时间、实验鼠出血量及切口长度,于术后不同时间点评价各组大鼠BBB评分和神经电生理检查判定脊髓功能障碍及造模成功与否,以免疫组织化学方法评价大鼠脊髓损伤程度.结果胸椎揭盖开窗组大鼠手术时间[(20±7)min]、术中出血量[(1.2±0.4)ml]及切口长度[(3.0±0.6) cm]优于传统蚕食法组大鼠[(47.0±10.0)min、(2.3±0.9) ml及(5.0±0.7)cm],感染率降低,死亡率由传统蚕食法的15％降低至胸椎揭盖开窗法的0,BBB评分、神经电生理检查,免疫组织化学法检查结果提示以胸椎揭盖开窗法制备大鼠脊髓损伤模型脊髓损伤程度与传统方法比较差异无统计学意义(P＞0.05).结论胸椎揭盖开窗法制备大鼠脊髓损伤模型可降低造模大鼠的感染率并提高存活率.
목적 창건1충신적제비대서척수손상모형적수술방법.방법 장실험대서분위4조:전통잠식법개창가수술조(A조)、흉추게개개창가수술조(B조)、전통잠식법개창척수타격조(C조)、흉추게개개창척수타격조(D조),분별기록수술시간、실험서출혈량급절구장도,우술후불동시간점평개각조대서BBB평분화신경전생리검사판정척수공능장애급조모성공여부,이면역조직화학방법평개대서척수손상정도.결과 흉추게개개창조대서수술시간[(20±7)min]、술중출혈량[(1.2±0.4)ml]급절구장도[(3.0±0.6) cm]우우전통잠식법조대서[(47.0±10.0)min、(2.3±0.9) ml급(5.0±0.7)cm],감염솔강저,사망솔유전통잠식법적15％강저지흉추게개개창법적0,BBB평분、신경전생리검사,면역조직화학법검사결과제시이흉추게개개창법제비대서척수손상모형척수손상정도여전통방법비교차이무통계학의의(P＞0.05).결론 흉추게개개창법제비대서척수손상모형가강저조모대서적감염솔병제고존활솔.
Objective To create a new surgery and equipment for creating spinal cord injury rat.Methods 80 rats are divided into 4 groups:group A:encroach on method for thoracic fenestration without contusing spinal cord,group B:en bloc resection on thoracic for fenestration without contusing spinal cord,group C:encroach on method with contusing method,group D:enbloc resection method with contusing method.Time,blood loss and the length of the incision were recorded.Basso-Beattie-Bresnahan (BBB) score,sensory evoked potentials (SEP) and motor evoked potentials (MEP) were tested at certain postoperative time point.Immunohistochemistry was performed to evaluate the injury severity.Results Group D rats showed better outcomes in intraoperative time (20.0 ±7.0) min,blood loss (1.2 ±0.4) ml and the length of incision (3.0 ± 0.6) cm than those of other groups.The mortality reduced from 15％ in group C to 0 in group D.Group D achieve similarly satisfied result in BBB score,SEP and MEP.Immunohistochemistry suggest en bloc resection method woud not affect the severity of spinal cord injury.Conclusion With en bloc resection of thoracic fenestration on thoracic creating spinal cord injury rats,lower infection rates and higher survival will be obtained.