CAJ | 학술논문

目的观察盐霉素对乳腺癌细胞株MCF-7、MCF-7/ADR、MDA-MB-231的体外增殖和侵袭能力的影响,探讨其对乳腺癌细胞的转移抑制作用.方法用细胞计数试剂盒(CCK-8)法检测细胞增殖,得出半数抑制浓度(IC50)值；用Transwell小室法检测侵袭能力的变化；分别用实时定量聚合酶链反应(Real-time PCR)法和Western blot法检测ETS1和基质金属蛋白酶(MMP)-1基因和蛋白的表达变化.结果盐霉素抑制乳腺癌细胞的增殖,3种细胞的IC50值分别为(7.47 ±0.64)、(5.11±0.97)、(4.08±0.85)μmol/L；经不同浓度的盐霉素处理后,3种细胞侵袭能力逐渐下降,分别为对照组的65.9％/41.9％、55.8％/37.8％、48.5％/24.6％；MCF-7/ADR和MDA-MB-231的ETS1和MMP-1表达水平随着盐霉素浓度升高而下降.结论盐霉素可以抑制乳腺癌细胞的增殖,降低其侵袭能力.
목적 관찰염매소대유선암세포주MCF-7、MCF-7/ADR、MDA-MB-231적체외증식화침습능력적영향,탐토기대유선암세포적전이억제작용.방법 용세포계수시제합(CCK-8)법검측세포증식,득출반수억제농도(IC50)치；용Transwell소실법검측침습능력적변화；분별용실시정량취합매련반응(Real-time PCR)법화Western blot법검측ETS1화기질금속단백매(MMP)-1기인화단백적표체변화.결과 염매소억제유선암세포적증식,3충세포적IC50치분별위(7.47 ±0.64)、(5.11±0.97)、(4.08±0.85)μmol/L；경불동농도적염매소처리후,3충세포침습능력축점하강,분별위대조조적65.9％/41.9％、55.8％/37.8％、48.5％/24.6％；MCF-7/ADR화MDA-MB-231적ETS1화MMP-1표체수평수착염매소농도승고이하강.결론 염매소가이억제유선암세포적증식,강저기침습능력.
Objective To study the effects of salinomycin on the proliferation and invasion of breast cancer cells and the mechanism.Methods The human breast cancer cells MCF-7,MCF-7/ADR and MDA-MB-231,were treated with salinomycin.Cell viability was determined by cell counting kit-8.The invasive ability of tumor cells was evaluated by Transwell chamber assay.The mRNA and protein levels of ETS1 and matrix metalloproteinase (MMP)-1 were detected by using real-time quantitative polymerase chain reaction (Real-time PCR) and Western blotting respectively.Results Salinomycin significantly inhibited the proliferation of breast cancer cells with the IC50 value being (7.47 ±0.64) for MCF-7,(5.11 ±0.97) for MCF-7/ADR,and (4.08 ±0.85) μmol/L for MDA-MB-231,respectively.The invasion capacity of breast cancer cells were significantly reduced by salinomycin (65.9％/41.9％ for MCR-7,55.8％ /37.8％ for MCR-7/ADR,and 48.5％/24.6％ for MDA-MB-23I,respectivel).Simustaneously,the expression levels of ETS1 and MMP-1 were downregulated by salinomycin.Conclusion Salinomycin can inhibit the proliferation of breast cancer cells,and reduce the invasion capacity.