CAJ | 학술논문

目的探讨键合表阿霉素纳米胶束(EPI-NPs)在大鼠移植性肝癌模型肝内的分布及药效.方法制备具有靶向控释作用的EPI-NPs,Walker-256细胞直接注射法制作大鼠移植性肝癌模型,通过肝动脉灌注给药(EPI含量均为2 mg/kg),利用组织荧光和匀浆荧光技术对比分析EPI-NPs和表阿霉素(EPI)在肝内的分布特点,并作药效对比.结果 EPI-NPs组肿瘤组织的EPI荧光强度高于肝组织(P＜0.05),而EPI组则表现为平均分布；EPI-NPs组在肿瘤及肝内的EPI荧光强度均高于EPI组(P＜0.05).EPI-NPs组大鼠肝移植瘤的体积抑瘤率及质量抑瘤率分别为72.96％和68.19％,EPI组的体积抑瘤率及质量抑瘤率分别为39.16％和35.33％,两者差异均有统计学意义(P＜0.05).EPI-NPs组生存率高于EPI组(P＜0.05).结论 EPI-NPs具有良好的肿瘤组织靶向性和抑瘤效果.
목적 탐토건합표아매소납미효속(EPI-NPs)재대서이식성간암모형간내적분포급약효.방법 제비구유파향공석작용적EPI-NPs,Walker-256세포직접주사법제작대서이식성간암모형,통과간동맥관주급약(EPI함량균위2 mg/kg),이용조직형광화균장형광기술대비분석EPI-NPs화표아매소(EPI)재간내적분포특점,병작약효대비.결과 EPI-NPs조종류조직적EPI형광강도고우간조직(P＜0.05),이EPI조칙표현위평균분포；EPI-NPs조재종류급간내적EPI형광강도균고우EPI조(P＜0.05).EPI-NPs조대서간이식류적체적억류솔급질량억류솔분별위72.96％화68.19％,EPI조적체적억류솔급질량억류솔분별위39.16％화35.33％,량자차이균유통계학의의(P＜0.05).EPI-NPs조생존솔고우EPI조(P＜0.05).결론 EPI-NPs구유량호적종류조직파향성화억류효과.
Objective To study the distribution and anti-tumor effect of epirubicin nanoparticles (EPI-NPs) in transplanted hepatocarcinoma rat model.Methods EPI-NPs with targeting and controlled release quality were prepared.The transplanted hepatocarcinoma rat models were made by direct injection of cancerous ascites with Walker-256 cells,and the drug was delivered to the rat model through hepatic arterial infusion (EPI content was 2 mg/kg).The distribution of EPI-NPs in the hepatocarcinoma rat model was observed by tissue fluorescence analysis and homogenate fluorescence analysis.The anti-tumor effect of EPI-NPs was also studied.Results The intensity of drug fluorescence in tumor tissue was stronger in EPI-NPs group than that in normal tissue at different time points (P ＜ 0.05).As to the EPI group,the fluorescence distributed equably in tumor and normal tissue.The intensity of drug fluorescence in tumor and intrahepatic tissue of EPI-NPs group was stronger than in EPIs group (P ＜ 0.05).The volume anti-tumor ratio and the quality anti-tumor ratio in EPI-NPs group were 72.96％ and 68.19％ respectively,which were 39.16％ and 35.33％ in EPI group (P ＜0.05).The survival rate in EPI-NPs group was higher than that in EPI group.Conclusion EPI-NPs were proved to have good tumor targeting essence and favourable tumor suppression effect.