CAJ | 학술논문

目的观察不同浓度全反式维甲酸(ATRA)与三氧化二砷(As2 O3)单独及联合作用于人肝癌细胞株HepG-2细胞,检测其体外生长增殖抑制率、细胞凋亡率及细胞内钙离子浓度([Ca2+]i)的变化,探讨其诱导肝癌细胞凋亡可能机制.方法分别及联合应用不同浓度的ATRA(0.1、1.0、10.0 μmol/L)、As2O3(0.5、1.0、2.0μmol/L)处理HepG-2细胞,用噻唑蓝(MTr)法测定不同作用时间细胞增长抑制率；流式细胞仪(FCM)膜联蛋白V/碘化丙锭(Annexin V/PI)双染色法检测细胞凋亡率的变化；采用荧光探针技术(Fluo-3),应用激光共聚焦显微镜,测定细胞内[Ca2+]i的变化.结果 ATRA、As2O3对人肝癌HepG-2细胞增殖有抑制作用,抑制率与药物浓度、作用时间呈正相关,联合用药优于单独用药；ATRA、As2O3能够诱导人肝癌细胞HepG-2的凋亡,随药物作用时间的延长、药物浓度的增高,细胞凋亡率逐渐增高,联合组更明显；作用时间为24、48 h时,细胞内Ca2+荧光强度与药物浓度、作用时间呈正相关,联合组荧光增强更明显,与对照组比较差异有统计学意义(P＜0.01),而作用时间至72 h时,荧光强度又恢复至正常水平(P＞0.05).结论 ATRA、As2O3能够诱导人肝癌细胞HepG-2凋亡,其诱导凋亡分子机制可能与细胞内钙离子浓度变化有关；ATRA、As2O3体外联合应用有协同抗肝癌作用.
목적 관찰불동농도전반식유갑산(ATRA)여삼양화이신(As2 O3)단독급연합작용우인간암세포주HepG-2세포,검측기체외생장증식억제솔、세포조망솔급세포내개리자농도([Ca2+]i)적변화,탐토기유도간암세포조망가능궤제.방법 분별급연합응용불동농도적ATRA(0.1、1.0、10.0 μmol/L)、As2O3(0.5、1.0、2.0μmol/L)처리HepG-2세포,용새서람(MTr)법측정불동작용시간세포증장억제솔；류식세포의(FCM)막련단백V/전화병정(Annexin V/PI)쌍염색법검측세포조망솔적변화；채용형광탐침기술(Fluo-3),응용격광공취초현미경,측정세포내[Ca2+]i적변화.결과 ATRA、As2O3대인간암HepG-2세포증식유억제작용,억제솔여약물농도、작용시간정정상관,연합용약우우단독용약；ATRA、As2O3능구유도인간암세포HepG-2적조망,수약물작용시간적연장、약물농도적증고,세포조망솔축점증고,연합조경명현；작용시간위24、48 h시,세포내Ca2+형광강도여약물농도、작용시간정정상관,연합조형광증강경명현,여대조조비교차이유통계학의의(P＜0.01),이작용시간지72 h시,형광강도우회복지정상수평(P＞0.05).결론 ATRA、As2O3능구유도인간암세포HepG-2조망,기유도조망분자궤제가능여세포내개리자농도변화유관；ATRA、As2O3체외연합응용유협동항간암작용.
Objective To observe inhibitory effects and the induction of differentiation on the human liver cancer cell line HepG-2 in vitro after induced by As2O3 alone or combined with all-trans retinoic acid (ATRA),and the change of intracellular Ca2+.Methods In vitro,HepG-2 cells were treated with ATRA (0.1,1.0,10.0 μ mol/L) and (or) As2O3(0.5,1.0,2.0 μmol/L).At24,48,and72 h,the cell growth inhibition rate was assayed by methyl thiazol tetrazolium (MTT) method.The apoptosis rate of HepG-2 cells was examined by using flow cytometry (FCM) with Annexin V/propidium iodide (PI) double stainings.The intracellular Ca2+ levels were determined under laser scanning confocal microscope.Results Mter induction by ATRA (0.1,1.0 and 10.0 mol/L) and (or) As2O3 (0.5,1.0 and 2.0 mol/L),the cell proliferation was inhibited in time-and dose-dependent manners.As compared with As2O3 or ATRA used alone,ATRA combined with As2O3 significantly inhibited the growth of HepG-2 cells.The apoptosis morphologic changes of HepG-2 cells treated with As2O3 and (or) ATRA were observed through Annexin V-PI staining.AnnexinV-PI staining showed that both As2O3 and ATRA could induce HepG-2 cell apoptosis and the apoptosis ratio was time-dependent (P ＜0.05).The combined use of As2O3 and ATRT could significantly increase the apoptosis rate (P ＜0.05).The concentration of intracellular Ca2+ in HepG-2 cells treated with As2O3 and (or) ATRA for 24 and 48 h was statistically different from that in the controls (P＜0.01).Laser scanning confocal microscopy revealed that both As2O3 and ATRA could induce HepG-2 cell apoptosis and the apoptosis rate was time-dependent.The combined use of As2 O3 and ATRT could significantly increase the concentration,but after 72 h,the concentration of intracellular Ca2 + was not stastically different from that in controls (P ＞ 0.05).Conclusion ATRA or As2O3 could inhibit the growth of human liver cancer cell line HepG-2 in a dose-and-time dependent manner.The molecular mechanism of inducing apoptosis of HepG-2 cells may be related to up-regulation of hypertonic solution on calcium in endothelial cells.ATRA combined with As2O3 could significantly increase anti-liver cancer effect synergically.