CAJ | 학술논문

目的观察小鼠原位肝癌移植模型中髓系来源抑制性细胞(MDSCs)的表达.方法将10只BALB/c小鼠随机分为两组:正常组和荷瘤组,后组是将H22肝癌细胞注射到肝左外叶,制作小鼠原位肝癌移植模型.10d后处死小鼠.流式细胞术检测两组小鼠外周血、骨髓、脾脏及肝脏组织中MDSCs的表达.结果荷瘤组小鼠外周血、骨髓和脾脏中的MDSCs比例为[(47.73±6.00)、(71.90±4.30)、(11.21±1.19)％],均明显高于正常组小鼠[(18.33±2.31)、(59.03±4.50)、(5.82±0.58)％]；正常小鼠肝组织中MDSCs占肝内白细胞6.5％,荷瘤小鼠肝癌组织、癌旁组织中MDSCs占肝内白细胞分别为43.8％和12.8％.结论在小鼠原位肝癌移植模型中,MDSCs表达明显上调,为研究MDSCs在肝癌发生发展中的作用提供了良好的动物模型.
목적 관찰소서원위간암이식모형중수계래원억제성세포(MDSCs)적표체.방법 장10지BALB/c소서수궤분위량조:정상조화하류조,후조시장H22간암세포주사도간좌외협,제작소서원위간암이식모형.10d후처사소서.류식세포술검측량조소서외주혈、골수、비장급간장조직중MDSCs적표체.결과 하류조소서외주혈、골수화비장중적MDSCs비례위[(47.73±6.00)、(71.90±4.30)、(11.21±1.19)％],균명현고우정상조소서[(18.33±2.31)、(59.03±4.50)、(5.82±0.58)％]；정상소서간조직중MDSCs점간내백세포6.5％,하류소서간암조직、암방조직중MDSCs점간내백세포분별위43.8％화12.8％.결론 재소서원위간암이식모형중,MDSCs표체명현상조,위연구MDSCs재간암발생발전중적작용제공료량호적동물모형.
Objective To investigate the expression of myeloid-derived suppressor cells (MDSCs)in an orthotopic transplantation liver tumour model in BALB/c mice.Methods Ten healthy BALB/c mice were randomly divided into two groups (n =5 each):normal group and tumor-bearing group.The murine hepatic cancer cell line H22 cells were transplanted into the left liver lobe of mice to establish an orthotopic transplantation liver tumor model.The mice were sacrificed at 10th day.The expression of MDSCs in peripheral blood,bone marrow,spleen and liver was analyzed by flow cytometry.Results The presence of CD11b+ Gr-1 + MDSCs was significantly increased in the peripheral blood,bone marrow,and spleen of tumor-bearing mice [(47.73 ±.6.00),(71.90 ±4.30),(11.21 ± 1.19)％] as compared with normal mice [(18.33 ±2.31),(59.03 ±4.50),(5.82 ±0.58)％].The percentage of CD11b+ Gr-1 + MDSCs of intrahepatic leukocytes in normal mice was 6.5％.However,the percentage of MDSCs of intrahepatic leukocytes in tumor and paracancerous tissue was up to 43.8％ and 12.8％ respectively.Conclusion MDSCs were elevated in an orthotopic transplantation tumor mouse model of hepatocellular carcinoma.The mouse model can serve to study the role of MDSCs in hepatocellular carcinoma development.