中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
10期
2073-2075
,共3页
张晓庆%王兆文%陈达伟%郭峰%彭志海%樊军卫
張曉慶%王兆文%陳達偉%郭峰%彭誌海%樊軍衛
장효경%왕조문%진체위%곽봉%팽지해%번군위
肝移植%他克莫司%细胞色素P4503A亚家族多肽5%白细胞介素-18%基因多态性
肝移植%他剋莫司%細胞色素P4503A亞傢族多肽5%白細胞介素-18%基因多態性
간이식%타극막사%세포색소P4503A아가족다태5%백세포개소-18%기인다태성
Liver transplantation%Tacrolimus%Cytochrome P450 3A family polypeptide 5%Interleukin-18%Polymorphism
目的 探讨肝移植供体细胞色素P4503A亚家族多肽5(CYP3A5)和白细胞介素-18(IL-18)基因多态性与受体术后他克莫司血药浓度/给药剂量的关系.方法 应用测序技术检测84例肝移植患者供体CYP3A5 rs776746和IL-18rs5744247位点单核苷酸多态性,分析基因多态性与他克莫司代谢表型(全血谷浓度/剂量比值,C/D,ng· ml-·mg-·kg-)的相关性.结果 肝移植术后,供体IL-18 rs5744247 GG+ GC基因型患者他克莫司C/D值[(163.1±100.8)ng·ml-1.mg-1·kg-1]明显高于CC基因型患者[116.7±78.2)ng.ml-1·mg-1·kg-1,P＜0.01].移植表达CYP3A5蛋白(*1基因型)供体的患者中,不同供体IL-18基因型患者C/D值(ng.ml-1·mg-1·kg-1) (GG+ GC:133.4±65.3;CC:88.3±39.9)差异有统计学意义(P＜0.05).结论 联合检测供体CYP3A5rs776746和IL-18 rs5744247基因型有助于指导他克莫司个体化用药.
目的 探討肝移植供體細胞色素P4503A亞傢族多肽5(CYP3A5)和白細胞介素-18(IL-18)基因多態性與受體術後他剋莫司血藥濃度/給藥劑量的關繫.方法 應用測序技術檢測84例肝移植患者供體CYP3A5 rs776746和IL-18rs5744247位點單覈苷痠多態性,分析基因多態性與他剋莫司代謝錶型(全血穀濃度/劑量比值,C/D,ng· ml-·mg-·kg-)的相關性.結果 肝移植術後,供體IL-18 rs5744247 GG+ GC基因型患者他剋莫司C/D值[(163.1±100.8)ng·ml-1.mg-1·kg-1]明顯高于CC基因型患者[116.7±78.2)ng.ml-1·mg-1·kg-1,P＜0.01].移植錶達CYP3A5蛋白(*1基因型)供體的患者中,不同供體IL-18基因型患者C/D值(ng.ml-1·mg-1·kg-1) (GG+ GC:133.4±65.3;CC:88.3±39.9)差異有統計學意義(P＜0.05).結論 聯閤檢測供體CYP3A5rs776746和IL-18 rs5744247基因型有助于指導他剋莫司箇體化用藥.
목적 탐토간이식공체세포색소P4503A아가족다태5(CYP3A5)화백세포개소-18(IL-18)기인다태성여수체술후타극막사혈약농도/급약제량적관계.방법 응용측서기술검측84례간이식환자공체CYP3A5 rs776746화IL-18rs5744247위점단핵감산다태성,분석기인다태성여타극막사대사표형(전혈곡농도/제량비치,C/D,ng· ml-·mg-·kg-)적상관성.결과 간이식술후,공체IL-18 rs5744247 GG+ GC기인형환자타극막사C/D치[(163.1±100.8)ng·ml-1.mg-1·kg-1]명현고우CC기인형환자[116.7±78.2)ng.ml-1·mg-1·kg-1,P＜0.01].이식표체CYP3A5단백(*1기인형)공체적환자중,불동공체IL-18기인형환자C/D치(ng.ml-1·mg-1·kg-1) (GG+ GC:133.4±65.3;CC:88.3±39.9)차이유통계학의의(P＜0.05).결론 연합검측공체CYP3A5rs776746화IL-18 rs5744247기인형유조우지도타극막사개체화용약.
Objective To investigate the effect of cytochrome P450 3A family polypeptide 5 (CYP3A5) and interleukin-18 (IL-18) polymorphisms on the concentration/dose ratio of tacrolimus in Chinese liver transplant patients.Methods Donor CYP3A5 polymorphisms at rs776746 and IL-18 polymorphisms at rs5744247 were assessed by polymerase chain reaction (PCR) amplification and DNA sequencing for 84 liver transplant patients.The relationship between gene polymorphism and tacrolimus concentration/dose ralio was analyzed.Results The tacrolimus C/D ratios(ng.ml-1 ·mg-1 · kg-1) of the recipients with donors who were IL-18 rs5744247 GG + GC (163.1 ± 100.8) genotype were higher than those with donors who were CC genotype (116.7 ± 78.2,P ＜ 0.001).The tacrolimus C/D ratios of the recipients with donors who were CYP3A5 expressors and had IL-18 rs5744247 GG + GC genotype (133.4 ± 65.3) were higher than those with donors who were CYP3A5 expressors and had IL-18 rs5744247 CC genotype (88.3 ± 39.9,P ＜ 0.05).Conclusion Determination of donor CYP3A5 and IL-18 genotype may be performed prospectively to help individualizing tacrolimus dose regimen for liver transplant patients.
