中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
10期
2092-2094
,共3页
乳腺肿瘤%Psammaplysene A%叉头转录因子O1A蛋白
乳腺腫瘤%Psammaplysene A%扠頭轉錄因子O1A蛋白
유선종류%Psammaplysene A%차두전록인자O1A단백
Breast neoplasms%Psammaplysene A%Forkhead transcription factors O1A
目的 探讨Psammaplysene A(PsA)诱导叉头转录因子O1A蛋白(FOXO1A)表达对人乳腺癌细胞株MCF-7及SKBR3增殖和凋亡的影响.方法 用PsA干预2株细胞后,激光共聚焦显微镜(CSM)观察FOXO1A蛋白在细胞中表达；噻唑蓝(MTT)法检测细胞增殖；流式细胞仪(FCM)分析细胞凋亡和周期变化；Western blot检测B细胞淋巴瘤/白血病-2蛋白家族促凋亡调节蛋白(Bim)表达.结果 FOXO 1A蛋白定位于胞核中,PsA诱导后其表达明显增加,细胞发生凋亡,生长减慢.分别用0.1 μmol/L和1.0 μmol/L的PsA干预2株细胞后,凋亡率分别为(16.3±1.4)％和(32.5±2.3)％；(13.7±1.9)％和(30.3±1.6)％；Bim蛋白增加,和阴性对照组比较差异有统计学意义(P＜0.01).结论 PsA通过诱导FOXO1A蛋白表达抑制了乳腺癌细胞增殖,促进凋亡,其机制可能与Bim蛋白激活有关.FOXO1A基因有望成为乳腺癌基因治疗的有效靶点.
目的 探討Psammaplysene A(PsA)誘導扠頭轉錄因子O1A蛋白(FOXO1A)錶達對人乳腺癌細胞株MCF-7及SKBR3增殖和凋亡的影響.方法 用PsA榦預2株細胞後,激光共聚焦顯微鏡(CSM)觀察FOXO1A蛋白在細胞中錶達；噻唑藍(MTT)法檢測細胞增殖；流式細胞儀(FCM)分析細胞凋亡和週期變化；Western blot檢測B細胞淋巴瘤/白血病-2蛋白傢族促凋亡調節蛋白(Bim)錶達.結果 FOXO 1A蛋白定位于胞覈中,PsA誘導後其錶達明顯增加,細胞髮生凋亡,生長減慢.分彆用0.1 μmol/L和1.0 μmol/L的PsA榦預2株細胞後,凋亡率分彆為(16.3±1.4)％和(32.5±2.3)％；(13.7±1.9)％和(30.3±1.6)％；Bim蛋白增加,和陰性對照組比較差異有統計學意義(P＜0.01).結論 PsA通過誘導FOXO1A蛋白錶達抑製瞭乳腺癌細胞增殖,促進凋亡,其機製可能與Bim蛋白激活有關.FOXO1A基因有望成為乳腺癌基因治療的有效靶點.
목적 탐토Psammaplysene A(PsA)유도차두전록인자O1A단백(FOXO1A)표체대인유선암세포주MCF-7급SKBR3증식화조망적영향.방법 용PsA간예2주세포후,격광공취초현미경(CSM)관찰FOXO1A단백재세포중표체；새서람(MTT)법검측세포증식；류식세포의(FCM)분석세포조망화주기변화；Western blot검측B세포림파류/백혈병-2단백가족촉조망조절단백(Bim)표체.결과 FOXO 1A단백정위우포핵중,PsA유도후기표체명현증가,세포발생조망,생장감만.분별용0.1 μmol/L화1.0 μmol/L적PsA간예2주세포후,조망솔분별위(16.3±1.4)％화(32.5±2.3)％；(13.7±1.9)％화(30.3±1.6)％；Bim단백증가,화음성대조조비교차이유통계학의의(P＜0.01).결론 PsA통과유도FOXO1A단백표체억제료유선암세포증식,촉진조망,기궤제가능여Bim단백격활유관.FOXO1A기인유망성위유선암기인치료적유효파점.
Objective To investigate the effects of psammaplysene A (PsA) induced forkhead transcription factors O1 A (FOXO1 A) expression in cell proliferation and apoptosis of human breast carcinoma cell lines.Methods Breast carcinoma cells were treated by PsA.Localization of FOXO1 A protein was analyzed by laser scanning confocal microscopy (CSM).Cell proliferation was evaluated by methyl thiazol tetrazolium (MTT) assay.Cell apoptosis was detected by flow cytometry (FCM).Expression of B-cell lymphoma/Leukemia-2 interacting mediator of cell death (Bim) and FOXO1A were detected by Western blot.Results FOXO1A protein located in cell nucleus,and FOXO1A expression level increased after PsA treatment.Cell viability significantly decreased in MCF-7 and SKBR3 cells.Apoptosis rate of MCF-7 and SKBR3 cellwas (16.3±1.4)％ and (32.5±2.3),(13.7±1.9)％ and (30.3±1.6)％ when exposed to 0.1,1 μmol/L PsA respectively.Meanwhile,protein levels of Bim and FOXO1A significantly increased comnpared to phosphate buffer (PBS) negative control of MCF-7 and SKBR3 cells (P ＜0.01).Conclusion Increase of FOXO1A expression after PsA treatment could inhibit proliferation and induce apoptosis in breast cancer cells.The possible mechanism relates to Bim activation induced by FOXO1A.FOXO1A gene is a promising target for gene therapy against breast cancer.
