中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
10期
2108-2110
,共3页
陈晖宽%杨春燕%郭日昌%吴士兴%包仕廷
陳暉寬%楊春燕%郭日昌%吳士興%包仕廷
진휘관%양춘연%곽일창%오사흥%포사정
胰腺癌%赖氨酰氧化酶样蛋白-2%侵袭%黏附
胰腺癌%賴氨酰氧化酶樣蛋白-2%侵襲%黏附
이선암%뢰안선양화매양단백-2%침습%점부
Pancreatic cancer%Lysyl oxidase-like 2 protein%Invasion%Adhension
目的 观察沉默赖氨酰氧化酶样蛋白-2(LOXL2)基因对胰腺癌细胞的影响.方法 利用脂质体法将构建好的LOXL2-短发卡RNA(shRNA)质粒转染到胰腺癌Panc-1细胞中,应用Western blot检测转染前后胰腺癌Panc-1细胞中LOXL2蛋白表达量的变化,Transwell侵袭实验检测胰腺癌细胞的体外侵袭力,MTT法检测胰腺癌细胞的黏附能力.结果 LOXL2-shRNA转染组Panc-1细胞中LOXL2蛋白表达水平(0.32±0.01)、体外侵袭力[(32.2±3.8)个]、黏附率[(75.4±3.2)％]较转染前[1.05±0.05、68.6±3.5、(90.3±2.1)％,P＜0.05]明显降低.结论 沉默LOXL2基因能抑制胰腺癌Panc-1细胞的侵袭能力和黏附能力.
目的 觀察沉默賴氨酰氧化酶樣蛋白-2(LOXL2)基因對胰腺癌細胞的影響.方法 利用脂質體法將構建好的LOXL2-短髮卡RNA(shRNA)質粒轉染到胰腺癌Panc-1細胞中,應用Western blot檢測轉染前後胰腺癌Panc-1細胞中LOXL2蛋白錶達量的變化,Transwell侵襲實驗檢測胰腺癌細胞的體外侵襲力,MTT法檢測胰腺癌細胞的黏附能力.結果 LOXL2-shRNA轉染組Panc-1細胞中LOXL2蛋白錶達水平(0.32±0.01)、體外侵襲力[(32.2±3.8)箇]、黏附率[(75.4±3.2)％]較轉染前[1.05±0.05、68.6±3.5、(90.3±2.1)％,P＜0.05]明顯降低.結論 沉默LOXL2基因能抑製胰腺癌Panc-1細胞的侵襲能力和黏附能力.
목적 관찰침묵뢰안선양화매양단백-2(LOXL2)기인대이선암세포적영향.방법 이용지질체법장구건호적LOXL2-단발잡RNA(shRNA)질립전염도이선암Panc-1세포중,응용Western blot검측전염전후이선암Panc-1세포중LOXL2단백표체량적변화,Transwell침습실험검측이선암세포적체외침습력,MTT법검측이선암세포적점부능력.결과 LOXL2-shRNA전염조Panc-1세포중LOXL2단백표체수평(0.32±0.01)、체외침습력[(32.2±3.8)개]、점부솔[(75.4±3.2)％]교전염전[1.05±0.05、68.6±3.5、(90.3±2.1)％,P＜0.05]명현강저.결론 침묵LOXL2기인능억제이선암Panc-1세포적침습능력화점부능력.
Objective To investigate the effects of silencing lysyl oxidase-like 2 protein (LOXL2)gene on pancreatic cancer cells.Methods LOXL2-short hairpin RNA (shRNA) plasmid was constructed by using lipofectamine,and transfected into pancreatic cancer Panc-1 cells.The changes of LOXL2 protein expression in pancreatic cancer panc-1 cells before and after transfection with LOXL2-shRNA were assayed by using Western blotting.Transwell invasion assay was used to assess the in vitro invasive ability of pancreatic cancer cells.Adhesive capacity of pancreatic cancer cells was measured by methyl thiazol tetrazolium (MTT) assay.Results The grey-scale value of LOXL2 protein in Panc-1 cells in LOXL2-shRNA transfection group was (0.32 ± 0.01),the number of migrating cells was (32.2 ± 3.8) and adhesive capacity was (75.4 ± 3.2)％,which were significantly reduced as compared with those before transfection [1.05 ± O.05,68.6 ± 3.5,and (90.3 ± 2.1) ％,P ＜ 0.05].Conclusion Silencing LOXL2 gene can inhibit the invasive ability and adhensive capacity of pancreatic cancer Panc-1 cells.
