CAJ | 학술논문

目的用最小的肾脏功能损伤筛选制作短时、经济和成石效果好的大鼠肾草酸钙结石模型.方法将雄性SD大鼠90只随机分为两组.实验1采用1.0％氯化铵和不同浓度乙二醇(EC,0.1％、0.2％、0.4％、0.8％)处理.实验2单用不同浓度EG(0.1％、0.2％、0.4％、0.8％)处理.7～21 d后处死大鼠,测体质量、血、尿肌酐和尿草酸盐含量.苏木素-伊红(HE)染色观察肾组织病理改变及肾成石.结果实验1中,不同浓度EG+ 1.0％氯化铵处理7d后,大鼠尿草酸盐排泄量均高于对照组(P＜0.05).用0.4％或0.8％ EG+ 1.0％氯化铵处理14d后,大鼠肌酐清除率均低于对照组(P＜0.01)；光镜观察,肾皮质、髓质和肾乳头等处出现草酸钙结晶体,肾组织炎性损伤加重.实验2中,用0.4％或0.8％ EG处理7d后,尿草酸盐排泄量高于对照组(P＜0.01),但肌酐清除率变化差异无统计学意义(P＞0.05).光镜观察,肾皮质及间质未出现明显水肿,可观察到少量结石结晶但无炎性细胞浸润.结论单用EG处理大鼠可以在没有严重肾损伤的情况下构造短时、经济和成石效果好的大鼠肾草酸钙结石模型.
목적 용최소적신장공능손상사선제작단시、경제화성석효과호적대서신초산개결석모형.방법 장웅성SD대서90지수궤분위량조.실험1채용1.0％록화안화불동농도을이순(EC,0.1％、0.2％、0.4％、0.8％)처리.실험2단용불동농도EG(0.1％、0.2％、0.4％、0.8％)처리.7～21 d후처사대서,측체질량、혈、뇨기항화뇨초산염함량.소목소-이홍(HE)염색관찰신조직병리개변급신성석.결과 실험1중,불동농도EG+ 1.0％록화안처리7d후,대서뇨초산염배설량균고우대조조(P＜0.05).용0.4％혹0.8％ EG+ 1.0％록화안처리14d후,대서기항청제솔균저우대조조(P＜0.01)；광경관찰,신피질、수질화신유두등처출현초산개결정체,신조직염성손상가중.실험2중,용0.4％혹0.8％ EG처리7d후,뇨초산염배설량고우대조조(P＜0.01),단기항청제솔변화차이무통계학의의(P＞0.05).광경관찰,신피질급간질미출현명현수종,가관찰도소량결석결정단무염성세포침윤.결론 단용EG처리대서가이재몰유엄중신손상적정황하구조단시、경제화성석효과호적대서신초산개결석모형.
Objective To screen a short-term,economical and satisfied model of renal calcium oxalate calculus in rats with minimal renal damage.Methods Ninety male Sprague-Dawley (SD) rats were randomly divided into two groups.In group 1,the rats were treated with 1.0％ ammonium chloride and different concentrations of ethylene glycol (0.1％,0.2％,0.4％ and 0.8％).In group 2,the rats were treated with different concentrations of ethylene glycol (0.1％,0.2％,0.4％ and 0.8％).The body mass change,the serum creatinine and urine creatinine,and urinary oxalate excretion were measured.Conventional HE dyeing was carried out for kidney tissues and pathological changes,and the status of kidney stone formation was further observed.Results In group 1 treated with 1.0％ ammonium chloride and 0.1％ ethylene glycol for 7 days,the urinary oxalate excretion days was significantly higher than in control group (P ＜0.05).In group 1 treated with 0.4％ or 0.8％ ethylene glycol + 1.0％ ammonium chloride for 14 days,the creatinine clearance was lower than in control group (P ＜ 0.01).Light microscopy showed that calcium oxalate crystals formed in renal cortex,medulla and renal papilla,and inflammatory damage of renal tissue was aggravated.In group 2 treated with 0.4％ or 0.8％ ethylene glycol for 7 days,the urine oxalate excretion was higher than in control group (P ＜0.01),however,there was no statistically significant difference in the creatinine clearance (P ＞ 0.05).Light microscopy showed no obvious interstitial edema in renal cortex and a small amount of stone crystals were seen with no inflammatory cells infiltration.Conclusion Ethylene glycol alone is a quich,economic and good calcium oxalate crystallized method in rats without severe renal damage.