CAJ | 학술논문

目的探讨铁调素(Hepcidin)在大鼠慢性移植肾肾病(CAN)动物模型中的表达变化及临床意义.方法以F344近交系大鼠为供体、Lewis近交系大鼠为受体,原位肾移植,建立20只CAN大鼠模型；于模型建立术前、术后1、2及4个月分别收集大鼠的静脉血和尿样,检测肾功能；酶联免疫吸附法(ELISA)检测血清、尿Hepcidin、血清白细胞介素-6(IL-6)和促红细胞生成素(EPO)的表达；并于移植后2个月及4个月分别处死大鼠10只,观察各组大鼠移植肾组织病理学变化.结果血清Hepcidin、IL-6表达随着移植术的时间逐渐增高,术前、术后1、2及4个月分别为(8.18±1.60)、(10.94±2.10)、(12.71 ±2.10)、(15.81±2.40) μg/L; (2.52±0.41)、(15.58 ±6.94)、(18.16±7.08)、(25.71 ±5.71) ng/L.尿Hepcidin排出逐渐减少,术前、术后l、2及4个月分别为(17.52±2.60)、(13.65 ±2.80)、(12.02±1.30)、(10.13 ±1.80) μg/L,EPO表达逐渐减少术前、术后1、2及4个月分别为(15.51 ±0.76)、(10.29 ±0.58)、(6.37 ±0.82)、(1.23±0.15) U/L,术前与术后比较差异有统计学意义(P＜0.05)；移植术后大鼠血肌酐(Cr)、尿素氮(BUN)逐渐升高,并且Cr与血清Hepcidin呈正相关；移植术后血清Hepcidin的表达与IL-6呈正相关,与EPO呈负相关；肾脏病理形态、HE染色符合CAN的病理改变.结论随着移植肾功能的减退,大鼠体内微炎性反应增加,Hepcidin在大鼠CAN模型表达变化与肾功能有关.Hepcidin随着时间和炎症状态表达的变化可反映肾功能损伤.
목적 탐토철조소(Hepcidin)재대서만성이식신신병(CAN)동물모형중적표체변화급림상의의.방법 이F344근교계대서위공체、Lewis근교계대서위수체,원위신이식,건립20지CAN대서모형；우모형건립술전、술후1、2급4개월분별수집대서적정맥혈화뇨양,검측신공능；매련면역흡부법(ELISA)검측혈청、뇨Hepcidin、혈청백세포개소-6(IL-6)화촉홍세포생성소(EPO)적표체；병우이식후2개월급4개월분별처사대서10지,관찰각조대서이식신조직병이학변화.결과 혈청Hepcidin、IL-6표체수착이식술적시간축점증고,술전、술후1、2급4개월분별위(8.18±1.60)、(10.94±2.10)、(12.71 ±2.10)、(15.81±2.40) μg/L; (2.52±0.41)、(15.58 ±6.94)、(18.16±7.08)、(25.71 ±5.71) ng/L.뇨Hepcidin배출축점감소,술전、술후l、2급4개월분별위(17.52±2.60)、(13.65 ±2.80)、(12.02±1.30)、(10.13 ±1.80) μg/L,EPO표체축점감소술전、술후1、2급4개월분별위(15.51 ±0.76)、(10.29 ±0.58)、(6.37 ±0.82)、(1.23±0.15) U/L,술전여술후비교차이유통계학의의(P＜0.05)；이식술후대서혈기항(Cr)、뇨소담(BUN)축점승고,병차Cr여혈청Hepcidin정정상관；이식술후혈청Hepcidin적표체여IL-6정정상관,여EPO정부상관；신장병리형태、HE염색부합CAN적병리개변.결론 수착이식신공능적감퇴,대서체내미염성반응증가,Hepcidin재대서CAN모형표체변화여신공능유관.Hepcidin수착시간화염증상태표체적변화가반영신공능손상.
Objective To summarize the expression and cinical significance of hepcidin in the rat experimental model for chronic allograft nephropathy.Methods We used Fisher rats as donors and Lewis rats as recipients.Twenty rat models for chronic allograft nephropathy were established.The blood and urine samples were collected before and 1,2 and 4 month(s) after operation,and renal functions were examined.Enzyme linked immunosorbent assay (ELISA) was used to detect serum and urine hepeidin,interleukin-6 (IL-6) and erythropoietin (EPO).The pathological changes of the renal tissues were observed 2 and 4 months after renal transplantation.Results Serum hepcidin and IL-6 were increased with time of operation (before and 1,2 and 4 months),they are respectively (8.18 ± 1.60),(10.94 ± 2.10),(12.71±2.10),(15.81 ±2.40) μg/L,and (2.52±0.41),(15.58±6.94),(18.16±7.08),(25.71 ± 5.71) ng/L; urine hepcidin excretion and EPO were gradually reduced,they are respectively (17.52±2.60),(13.65 ±2.80),(12.02 ±1.30),(10.13±1.80) μg/L,and (15.51±0.76),(10.29 ±0.58),(6.37 ±0.82),(1.23 ±0.15) U/L.There was significant difference before and after operation.The Scr and BUN demonstrated a significant increase after operation,Scr and IL-6 were positively correlated with serum hepcidin,and EPO was negatively correlated with serum hepcidin.After renal transplantation,renal pathological changes were consistent with those of chronic allograft nephropathy.Conclusion (1) With the deteriation of renal function,inflammatory reaction increased in vivo in the rat models.Hepcidin was correlated with renal function; (2) The expression of hepcidin can be used to reflect injured renal function along with time and inflammatory reaction.