CAJ | 학술논문

目的观察负压封闭引流技术联合游离植骨方法治疗骨缺损的效果及其血管化的影响.方法建立双侧兔桡骨缺损的模型,通过创面一般愈合情况、X线、苏木素-伊红(HE)染色、免疫组织化学及实时定量聚合酶链反应(Real-time PCR)检测来评价效果.结果实验组术后创面肉芽质量及愈合率好于对照组,恢复时间小于对照组,4周时X线示骨折愈合率显著高于对照组,HE染色血管组织较对照组丰富；血管内皮生长因子(VEGF)免疫组织化学及实时定量PCR结果均明显高于对照组,差异均有统计学意义(P＜0.05).结论负压封闭引流联合游离植骨可以有效的修复骨与软组织复合缺损,其机制可能是通过上调VEGF表达在促进游离植骨血管化来实现的.
목적 관찰부압봉폐인류기술연합유리식골방법치료골결손적효과급기혈관화적영향.방법 건립쌍측토뇨골결손적모형,통과창면일반유합정황、X선、소목소-이홍(HE)염색、면역조직화학급실시정량취합매련반응(Real-time PCR)검측래평개효과.결과 실험조술후창면육아질량급유합솔호우대조조,회복시간소우대조조,4주시X선시골절유합솔현저고우대조조,HE염색혈관조직교대조조봉부；혈관내피생장인자(VEGF)면역조직화학급실시정량PCR결과균명현고우대조조,차이균유통계학의의(P＜0.05).결론 부압봉폐인류연합유리식골가이유효적수복골여연조직복합결손,기궤제가능시통과상조VEGF표체재촉진유리식골혈관화래실현적.
Objective To study the feasibility and efficiency of vacuum sealing drainage (VSD) technology combined with bone graft and its influence on revascularization.Methods Twenty-four rabbits with bone and soft tissue defects were randomly divided into experimental group (VSD therapy with open bone graft) and control group (only open bone graft).The curative efficacy was assessed by wound conditions and wound healing time,X-ray examination,immunohistochemistry,and real-time quantitative polymerase chain reaction (Real-time PCR).Results Wounds and bone defects in experimental group tended to have shorter healing time and healthier wound conditions.Hematoxylin and eosin (HE) staining showed that the graft in experimental group produced plentiful micrangium.The expression of vascular endothelial growth factor (VEGF) via immunohistochemistry and Real-time PCR was significantly higher in experimental group than in control group.Conclusion The VSD therapy combined with open bone graft can be used as a feasible and valuable method to treat composite bone and soft tissue defect,and it may promote revascularization of the graft through up-regulating the expression of VEGF.