中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
10期
2201-2202
,共2页
乳腺癌%外周血%T细胞%流式细胞术
乳腺癌%外週血%T細胞%流式細胞術
유선암%외주혈%T세포%류식세포술
Breast cancer%Peripheral blood%T cells%Flow cytometry
目的 探讨乳腺癌患者外周血和肿瘤组织CD4+ CD25+ CD127low/-T细胞(Tregs)的变化及其临床意义.方法 采用流式细胞术对48例乳腺癌患者及12例健康志愿者外周血Treg细胞进行检测,同时对10例乳腺癌患者癌组织及癌旁组织Treg细胞进行检测.结果 10例乳腺癌患者癌组织Treg细胞表达为(39.74 ±9.02)％,显著高于其癌旁组织的表达[(29.81±8.52)％,P＜0.05].48例乳腺癌患者外周血Treg细胞表达为(7.04±1.56)％,显著高于12例健康志愿者外周血Treg细胞的表达[(4.01±1.05)％,P＜0.05].且随着疾病的进展,外周血Treg细胞水平升高,Ⅱ期+Ⅲ期乳腺癌患者外周血Treg细胞表达为(8.15±1.28)％,显著高于Ⅰ期患者[(6.39±0.81)％,P＜0.05]和健康志愿者[(4.01±1.05)％,P＜0.05].结论 Treg细胞在乳腺癌患者外周血和肿瘤组织的高表达,可能是肿瘤长期免疫逃逸的重要原因,其外周血数量与乳腺癌进展密切相关,越晚期表达水平越高.
目的 探討乳腺癌患者外週血和腫瘤組織CD4+ CD25+ CD127low/-T細胞(Tregs)的變化及其臨床意義.方法 採用流式細胞術對48例乳腺癌患者及12例健康誌願者外週血Treg細胞進行檢測,同時對10例乳腺癌患者癌組織及癌徬組織Treg細胞進行檢測.結果 10例乳腺癌患者癌組織Treg細胞錶達為(39.74 ±9.02)％,顯著高于其癌徬組織的錶達[(29.81±8.52)％,P＜0.05].48例乳腺癌患者外週血Treg細胞錶達為(7.04±1.56)％,顯著高于12例健康誌願者外週血Treg細胞的錶達[(4.01±1.05)％,P＜0.05].且隨著疾病的進展,外週血Treg細胞水平升高,Ⅱ期+Ⅲ期乳腺癌患者外週血Treg細胞錶達為(8.15±1.28)％,顯著高于Ⅰ期患者[(6.39±0.81)％,P＜0.05]和健康誌願者[(4.01±1.05)％,P＜0.05].結論 Treg細胞在乳腺癌患者外週血和腫瘤組織的高錶達,可能是腫瘤長期免疫逃逸的重要原因,其外週血數量與乳腺癌進展密切相關,越晚期錶達水平越高.
목적 탐토유선암환자외주혈화종류조직CD4+ CD25+ CD127low/-T세포(Tregs)적변화급기림상의의.방법 채용류식세포술대48례유선암환자급12례건강지원자외주혈Treg세포진행검측,동시대10례유선암환자암조직급암방조직Treg세포진행검측.결과 10례유선암환자암조직Treg세포표체위(39.74 ±9.02)％,현저고우기암방조직적표체[(29.81±8.52)％,P＜0.05].48례유선암환자외주혈Treg세포표체위(7.04±1.56)％,현저고우12례건강지원자외주혈Treg세포적표체[(4.01±1.05)％,P＜0.05].차수착질병적진전,외주혈Treg세포수평승고,Ⅱ기+Ⅲ기유선암환자외주혈Treg세포표체위(8.15±1.28)％,현저고우Ⅰ기환자[(6.39±0.81)％,P＜0.05]화건강지원자[(4.01±1.05)％,P＜0.05].결론 Treg세포재유선암환자외주혈화종류조직적고표체,가능시종류장기면역도일적중요원인,기외주혈수량여유선암진전밀절상관,월만기표체수평월고.
Objective To investigate the expression and clinical signifycance of CD4 + CD25 + CD127low/-regulatory T cells (Tregs) in peripheral blood and tumor tissue of breast cancer patients.Methods The percentage of Tregs in tumor tissues,and adjacent tissues from 10 patients with breast cancer,peripheral blood from 48 patients with breast cancer and 12 healthy volunteers was detected by using flow cytometry.Results The percentage of Tregs in 10 breast cancer patients was (39.74 ± 9.02) ％ in tumor tissues,significantly higher than that in the adjacent tissues [(29.81 ± 8.52)％,P ＜0.05].The percentage of Tregs in peripheral blood of 48 breast cancer patients was (7.04 ± 1.56) ％,significantly higher than in 12 healthy volunteers [(4.01 ± 1.05) ％,P ＜ 0.05].With the progress of the disease,the proportion of Tregs in peripheral blood of breast cancer patients had a higher level.The levels of Tregs in peripheral blood of breast cancer patients with stage (Ⅱ + Ⅲ) were signifycantly higher than those of patients with stage Ⅰ [(6.39 ± 0.81) ％,P ＜ 0.05] and heathy volunteers [(4.01 ± 1.05) ％,P ＜ 0.05].Conclusion There is high expression of Tregs in peripheral blood and tumor tissues of patients with breast cancer,which might be closely related to long-term immunoescape of tumor.Besides,the populations of Tregs in peripheral blood may contributed to the progression of breast cancer.
