中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
10期
2215-2217
,共3页
周建炜%汤钊猷%卢创新%任正刚%乐凡%周云%罗执芬%崔勇霞%王朝杰
週建煒%湯釗猷%盧創新%任正剛%樂凡%週雲%囉執芬%崔勇霞%王朝傑
주건위%탕쇠유%로창신%임정강%악범%주운%라집분%최용하%왕조걸
癌,肝细胞%原位移植%射频消融%模型,动物
癌,肝細胞%原位移植%射頻消融%模型,動物
암,간세포%원위이식%사빈소융%모형,동물
Carcinoma,hepatocellular%Orthotopic implantation%Radiofrequency ablation%Model,animal
目的 建立稳定有效的转移性人肝癌裸小鼠原位种植合并姑息性射频消融模型.方法 采用60只BALB/c-nu/nu裸小鼠,建立转移性人肝癌裸小鼠原位种植模型；随机分为两组,移植瘤术后30 d进行姑息性射频消融术和仅开腹(假手术组).两组中各随机抽出7只裸鼠,在2次手术后4周处死裸鼠,观察肝脏移植瘤大小、坏死范围并行免疫组织化学染色检测血管内皮生长因子(VEGF)和基质金属蛋白酶(MMP)-2蛋白的表达.结果 人肝癌裸小鼠原位种植成瘤率为100.0％ (60/60)；姑息性射频消融成功率为96.7％ (29/30)；与假手术组比较,姑息性射频消融组移植瘤组织VEGF和MMP-2蛋白表达降低(P＜0.01).结论 转移性人肝癌裸小鼠原位种植合并姑息性射频消融模型手术成功率高,移植瘤组织VEGF和MMP-2蛋白表达降低.
目的 建立穩定有效的轉移性人肝癌裸小鼠原位種植閤併姑息性射頻消融模型.方法 採用60隻BALB/c-nu/nu裸小鼠,建立轉移性人肝癌裸小鼠原位種植模型；隨機分為兩組,移植瘤術後30 d進行姑息性射頻消融術和僅開腹(假手術組).兩組中各隨機抽齣7隻裸鼠,在2次手術後4週處死裸鼠,觀察肝髒移植瘤大小、壞死範圍併行免疫組織化學染色檢測血管內皮生長因子(VEGF)和基質金屬蛋白酶(MMP)-2蛋白的錶達.結果 人肝癌裸小鼠原位種植成瘤率為100.0％ (60/60)；姑息性射頻消融成功率為96.7％ (29/30)；與假手術組比較,姑息性射頻消融組移植瘤組織VEGF和MMP-2蛋白錶達降低(P＜0.01).結論 轉移性人肝癌裸小鼠原位種植閤併姑息性射頻消融模型手術成功率高,移植瘤組織VEGF和MMP-2蛋白錶達降低.
목적 건립은정유효적전이성인간암라소서원위충식합병고식성사빈소융모형.방법 채용60지BALB/c-nu/nu라소서,건립전이성인간암라소서원위충식모형；수궤분위량조,이식류술후30 d진행고식성사빈소융술화부개복(가수술조).량조중각수궤추출7지라서,재2차수술후4주처사라서,관찰간장이식류대소、배사범위병행면역조직화학염색검측혈관내피생장인자(VEGF)화기질금속단백매(MMP)-2단백적표체.결과 인간암라소서원위충식성류솔위100.0％ (60/60)；고식성사빈소융성공솔위96.7％ (29/30)；여가수술조비교,고식성사빈소융조이식류조직VEGF화MMP-2단백표체강저(P＜0.01).결론 전이성인간암라소서원위충식합병고식성사빈소융모형수술성공솔고,이식류조직VEGF화MMP-2단백표체강저.
Objective To establish the metastatic model of human hepatocelular carcinoma (HCC) in nude mice via orthotopic transplantation and further to palliative radiofrequency ablation (RFA) so as to provide a practicable model for the study of biological effects of RFA.Methods Human HCC cell line MHCC97-L was inoculated into 60 BALB/c nude mice.All these cases were divided equally into palliative RFA and sham-operation subgroups.Four weeks after the second operation,the tumor volumes,necrosis range and immunohistochemical staining of vascular endothelial growth factor (VEGF) and matrix metalloproteinase (MMP)-2 were measured in 7 randomly selected nude mice of each subgroup.Results All nude mice with xenograft tumors exhibited 100.0％ (60/60) transplantability,and 96.7％ (29/30) palliative RFA successful rate was achieved.The expression levels of VEGF and MMP-2 were significantly lower in palliative RFA group than in sham operation group (P ＜ 0.01).Conclusion Metastatic human hepatocellular carcinoma orthotopic transplantation model in combination with palliative RFA in nude mice has a high success rate.After orthotopie tumor transplantation,palliative RFA could reduce the expression of VEGF and MMP-2.
