中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
11期
2315-2317
,共3页
陈炜%江从庆%刘先桂%张志远%刘韦成%丁召%钱群%刘志苏
陳煒%江從慶%劉先桂%張誌遠%劉韋成%丁召%錢群%劉誌囌
진위%강종경%류선계%장지원%류위성%정소%전군%류지소
重组腺病毒载体%Galectin-1
重組腺病毒載體%Galectin-1
중조선병독재체%Galectin-1
Recombinant adenovirus vector%Galectin-1
目的 构建及鉴定重组腺病毒载体pAdxsi-GFP-Galectin-1.方法 通过Kpn I/BamH I双酶切从原始质粒获取半乳糖凝集素-1完整基因片段并连接到pSbuttle-CMV-EGFP重组穿梭载体；通过I-Ceu I+I-Sce I双酶切获取CMV-GFP-Galectin-1完整基因片段并连接到pAdxsi载体上,得到重组腺病毒载体pAdxsi-GFP-Galectin-1.结果 通过基因测序、酶切鉴定证实目的基因克隆至重组腺病毒载体,目的基因序列与Genebank录入序列一致.结论 成功构建重组腺病毒载体pAdxsi-GFP-Galectin-1.
目的 構建及鑒定重組腺病毒載體pAdxsi-GFP-Galectin-1.方法 通過Kpn I/BamH I雙酶切從原始質粒穫取半乳糖凝集素-1完整基因片段併連接到pSbuttle-CMV-EGFP重組穿梭載體；通過I-Ceu I+I-Sce I雙酶切穫取CMV-GFP-Galectin-1完整基因片段併連接到pAdxsi載體上,得到重組腺病毒載體pAdxsi-GFP-Galectin-1.結果 通過基因測序、酶切鑒定證實目的基因剋隆至重組腺病毒載體,目的基因序列與Genebank錄入序列一緻.結論 成功構建重組腺病毒載體pAdxsi-GFP-Galectin-1.
목적 구건급감정중조선병독재체pAdxsi-GFP-Galectin-1.방법 통과Kpn I/BamH I쌍매절종원시질립획취반유당응집소-1완정기인편단병련접도pSbuttle-CMV-EGFP중조천사재체；통과I-Ceu I+I-Sce I쌍매절획취CMV-GFP-Galectin-1완정기인편단병련접도pAdxsi재체상,득도중조선병독재체pAdxsi-GFP-Galectin-1.결과 통과기인측서、매절감정증실목적기인극륭지중조선병독재체,목적기인서렬여Genebank록입서렬일치.결론 성공구건중조선병독재체pAdxsi-GFP-Galectin-1.
Objective To construct and identify the recombinant adenovirus vector pAdxsi-GFP-Galectin-1.Methods Whole sequence of galectin-1 was obtained from original plasmid double-digested with Kpn I/BamH I enzymes.Then Galectin-1 DNA segments were linked into pShuttle-CMV-EGFP vector to obtain recombinant pShuttle-GFP-Galectin-1 plasmid.Next,recombinant DNA sequences CMV-GFP-Galectin1were double-digested with I-Ceu I/I-Sce I enzymes and constructed into pShuttle-GFP-Galectin-1 vector to establish recombinant adenovirus vector pAdxsi-GFP-Galectin-1.Results Through endonuclease and gene sequencing the target gene was verified to be corrrctly cloned in adenvrius vector.Conclusion The recombinant adenovirus vector pAdxsi-GFP-Galectin-1 was successfully constructed.
