CAJ | 학술논문

目的探讨抑癌基因乳腺癌转移抑制基因1(BRMS1)影响胶质瘤细胞侵袭的机制.方法将真核表达质粒pEGFP-BRMS1和对照质粒pEGFP-N2分别转入人胶质瘤U251和U87细胞中,Western blot技术检测BRMS1蛋白表达,人工基底膜侵袭实验检测细胞侵袭能力,明胶酶谱实验检测胶质瘤细胞分泌基质金属蛋白酶-2(MMP-2)的活性,Western blot检测金属蛋白酶组织抑制因子-2(TIMP-2)及MMP-2蛋白表达.结果与对照组比较,转染pEGFP-BRMS1质粒24 h后U251和U87细胞侵袭力分别下降了43％和52％,差异均有统计学意义(P＜0.01)；转染BRMS1可以明显抑制2种胶质瘤细胞分泌MMP-2活性；pEGFP-BRMS1可以通过上调TIMP-2的表达从而抑制MMP-2蛋白表达.结论 BRMS1可以通过增加TIMP-2表达进而抑制MMP-2的表达,打破TIMP-2/MMP-2平衡,最终抑制胶质瘤细胞侵袭能力.
목적 탐토억암기인유선암전이억제기인1(BRMS1)영향효질류세포침습적궤제.방법 장진핵표체질립pEGFP-BRMS1화대조질립pEGFP-N2분별전입인효질류U251화U87세포중,Western blot기술검측BRMS1단백표체,인공기저막침습실험검측세포침습능력,명효매보실험검측효질류세포분비기질금속단백매-2(MMP-2)적활성,Western blot검측금속단백매조직억제인자-2(TIMP-2)급MMP-2단백표체.결과 여대조조비교,전염pEGFP-BRMS1질립24 h후U251화U87세포침습력분별하강료43％화52％,차이균유통계학의의(P＜0.01)；전염BRMS1가이명현억제2충효질류세포분비MMP-2활성；pEGFP-BRMS1가이통과상조TIMP-2적표체종이억제MMP-2단백표체.결론 BRMS1가이통과증가TIMP-2표체진이억제MMP-2적표체,타파TIMP-2/MMP-2평형,최종억제효질류세포침습능력.
Objective To investigate the effect and mechanism of breast cancer metastasis suppressor-1 (BRMS1) on the human glioma cells invasion.Methods Transfection of the pEGFP-BRMS1 plasmids into the U251 and U87 glioma cells were carried out using Lipofectamine 2000.We studied the role of BRMS1 in glioma cell invasion by matrigel cell invasion assay.We performed gelatin zymography to measure the matrix metalloproteinase (MMP)-2 activities in glioma cells and Western blotting to examine the tissue inhibitorof metalloproteinase-2 (TIMP-2) and MMP-2 proteins expression after BRMS1 restoration.Results Compared to the control plasmids,pEGFP-BRMS1 plasmids could increase the expression of BRMS1 in both glioma cells significantly.In cell invasion assay,BRMS1 inhibits cell invasion ability of U251 and U87 cells in matrigel-coated Boyden chamber by 43％ and 52％,respectively.BRMS1 also suppress the MMP-2 activity.Increasing of BRMS1 expression upregulated TIMP-2 protein expression and inhibited MMP-2 protein expression.Conclusion Our data indicate that BRMS1 may be reduced glioma cells invasion abilities by the imbalance between TIMP-2 and MMP-2.