CAJ | 학술논문

目的观察过表达磷脂酰肌醇蛋白聚糖-5(GPC5)对肺鳞癌细胞株SK-MES-1增殖、迁移和侵袭能力的影响.方法构建慢病毒载体过表达GPC5-SK-MES-1和阴性对照NC-SK-MES-1稳转细胞株.通过细胞划痕和Transwell小室实验观察过表达GPC5对肺鳞癌细胞株SK-MES-1迁移和侵袭能力的影响.细胞计数试剂盒(CCK-8)法检测过表达GPC5对细胞增殖能力的影响,流式细胞仪检测其细胞周期的变化.结果与阴性对照组NC-SK-MES-1细胞比较,过表达GPC5的肺鳞癌细胞的GPC5基因表达量(79.113±9.246)增加了86倍.其细胞迁移间距(729.89±46.15)较对照组的(438.33±19.77)显著增加；细胞穿膜数[(33±3)个]较对照组[(128±3)个]显著减少；细胞相对增殖活性较对照组显著降低；细胞周期检测显示S期细胞数比率[(50.32±0.97)％]较对照组[(32.59±1.54)％]显著增加,差异均有统计学意义(P＜0.05).结论上调肺鳞癌细胞GPC5的表达水平能够有效抑制肺鳞癌细胞的增殖、迁移和侵袭能力.
목적 관찰과표체린지선기순단백취당-5(GPC5)대폐린암세포주SK-MES-1증식、천이화침습능력적영향.방법 구건만병독재체과표체GPC5-SK-MES-1화음성대조NC-SK-MES-1은전세포주.통과세포화흔화Transwell소실실험관찰과표체GPC5대폐린암세포주SK-MES-1천이화침습능력적영향.세포계수시제합(CCK-8)법검측과표체GPC5대세포증식능력적영향,류식세포의검측기세포주기적변화.결과 여음성대조조NC-SK-MES-1세포비교,과표체GPC5적폐린암세포적GPC5기인표체량(79.113±9.246)증가료86배.기세포천이간거(729.89±46.15)교대조조적(438.33±19.77)현저증가；세포천막수[(33±3)개]교대조조[(128±3)개]현저감소；세포상대증식활성교대조조현저강저；세포주기검측현시S기세포수비솔[(50.32±0.97)％]교대조조[(32.59±1.54)％]현저증가,차이균유통계학의의(P＜0.05).결론 상조폐린암세포GPC5적표체수평능구유효억제폐린암세포적증식、천이화침습능력.
Objective To investigate the effects of the up-regulated expression of glypican-5 (GPC5) on the proliferation,migration and invasion of lung squamous carcinoma cell line SK-MES-1.Methods Lentivirus-induced GPC5 over-expression was used for in vitro functional analyses in lung squamous carcinoma cell line SK-MES-1,including migration and invasive assay,proliferation assay as well as cell cycle analysis.Results Compared to the control,the expression of GPC5 was increased by 86 times in GPC5-SK-MES-1,whose length of cell migration spacing was longer [(729.89 ±46.15) vs.(438.33 ±19.77)] and number of penetrating the membrane was significantly reduced [(33 ± 3) vs.(128 ± 3)].The relative cell proliferation activity was significantly decreased,and the cell cycle analysis showed that the ratio of S-phase cells was markedly increased in GPC5-SK-MES-1 cells as compared with controls [(50.32 ± 0.97) ％ vs.(32.59 ± 1.54) ％] (P ＜ 0.05).Conclusion Up-regulation of GPC5 can inhibit the proliferation,migration and invasion of lung squamous carcinoma cells.