中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
11期
2361-2363
,共3页
谢华%农鲁明%高共鸣%周栋%何劲%陈旭霞
謝華%農魯明%高共鳴%週棟%何勁%陳旭霞
사화%농로명%고공명%주동%하경%진욱하
磷脂酶C-γ1%周期性应力%髓核细胞
燐脂酶C-γ1%週期性應力%髓覈細胞
린지매C-γ1%주기성응력%수핵세포
Phospholipase C-γ1 protein%Periodic mechanical stress%Nucleus pulposus cell
目的 观察周期性应力下磷脂酶C-γ1(PLCγ1)及其抑制剂对大鼠髓核细胞增殖的影响.方法 将细胞玻片随机分成4组:对照组、加压0.5h组、加压1h组、加压2h组.Western blot法检测各组PLCγ1、磷酸化PLCγ1表达.另取细胞玻片,随机分为3组:对照组、加压6h组、U73122+加压6h组.使用荧光实时定量聚合酶链反应(FQ-PCR)检测各组细胞外基质的表达.结果 PLCγ1磷酸化水平在加压0.5h组(0.109 ±0.010)、加压1h组(0.138±0.001)、加压2h组(0.201 ±0.014)较对照组(0.086 ±0.005)增高.加入U73122后,细胞外基质的基因表达,以对照组最低,加压6h组最高,U73122+加压6h组居中,差异有统计学意义(P<0.05).结论 周期性压力能促进髓核细胞的增殖,PLCγ1在压力传导中起信号传递作用.
目的 觀察週期性應力下燐脂酶C-γ1(PLCγ1)及其抑製劑對大鼠髓覈細胞增殖的影響.方法 將細胞玻片隨機分成4組:對照組、加壓0.5h組、加壓1h組、加壓2h組.Western blot法檢測各組PLCγ1、燐痠化PLCγ1錶達.另取細胞玻片,隨機分為3組:對照組、加壓6h組、U73122+加壓6h組.使用熒光實時定量聚閤酶鏈反應(FQ-PCR)檢測各組細胞外基質的錶達.結果 PLCγ1燐痠化水平在加壓0.5h組(0.109 ±0.010)、加壓1h組(0.138±0.001)、加壓2h組(0.201 ±0.014)較對照組(0.086 ±0.005)增高.加入U73122後,細胞外基質的基因錶達,以對照組最低,加壓6h組最高,U73122+加壓6h組居中,差異有統計學意義(P<0.05).結論 週期性壓力能促進髓覈細胞的增殖,PLCγ1在壓力傳導中起信號傳遞作用.
목적 관찰주기성응력하린지매C-γ1(PLCγ1)급기억제제대대서수핵세포증식적영향.방법 장세포파편수궤분성4조:대조조、가압0.5h조、가압1h조、가압2h조.Western blot법검측각조PLCγ1、린산화PLCγ1표체.령취세포파편,수궤분위3조:대조조、가압6h조、U73122+가압6h조.사용형광실시정량취합매련반응(FQ-PCR)검측각조세포외기질적표체.결과 PLCγ1린산화수평재가압0.5h조(0.109 ±0.010)、가압1h조(0.138±0.001)、가압2h조(0.201 ±0.014)교대조조(0.086 ±0.005)증고.가입U73122후,세포외기질적기인표체,이대조조최저,가압6h조최고,U73122+가압6h조거중,차이유통계학의의(P<0.05).결론 주기성압력능촉진수핵세포적증식,PLCγ1재압력전도중기신호전체작용.
Objective To evaluate the effect of the phospholipase C-γ1 (PLCγ1) protein and its inhibitor on rat nucleus pulposus cells proliferation under periodic mechanical stress.Methods Some cell slides were randomized into four groups:the control group,0.5 h stress group,1 h stress group and 2 h stress group.The expression of PLCγ1 and pPLCγ1 was detected by using Western blotting.The other slides were randomized into three groups:the control group,6 h stress group and U73122 + 6 h stress group.Type Ⅱ collagen and aggrecan gene expression levels were evaluated through real-time fluorescent quantitative polymerase-chain reaction.Results The pPLCγ1/PLCγ1 ratio in 0.5 h stress group (0.109 ± 0.010),1 h stress group (0.138 ±0.001) and 2 h stress group (0.201 ±0.014) was significantly higher than in control group (0.086 ± 0.005).The gene expression levels of type Ⅱ collagen and aggrecan in U73122 + 6 h stress group were significantly higher than those in control group,but lower than those in 6 h stress group (P < 0.05).Conclusion Periodic mechanical stress can promote the proliferation of rat nucleus pulposus cells.PLCγ1 plays important roles in the mechanical transductions.