CAJ | 학술논문

目的检测微小RNA(miRNA,miR)-21在甲状腺乳头状癌(PTC)、结节性甲状腺肿(NG)和癌旁正常甲状腺组织(NTT)中的表达差异,探讨miRNA-21在甲状腺乳头状癌中表达的意义.方法收集自2011年4月至2012年8月于我院甲状腺外科行甲状腺手术的30例患者的甲状腺乳头状癌组织及癌旁远端非瘤正常甲状腺组织,其中男8例,女22例,年龄14 ～ 73岁.结节性甲状腺肿15例.术中取材,立即放入液氮中保存后转入-70℃保存.采用实时定量逆转录-聚合酶链反应(RT-qPCR)检测miR-21在甲状腺癌组织及非肿瘤组织样本中的表达,并分析其与甲状腺乳头状癌临床病理特征的相关性.结果 miR-21在甲状腺乳头状癌中表达量明显高于非肿瘤组织(包括癌旁正常组织、结节性甲状腺肿)(P＜0.01),非肿瘤组织间比较差异无统计学意义(P＞0.05).miR-21的表达与甲状腺乳头状癌腺外浸润明显相关(P＜0.05).结论 miR-21的表达在甲状腺乳头状癌和非癌组织中存在着明显差异,miR-21的表达与甲状腺乳头状癌腺外浸润有关.
목적 검측미소RNA(miRNA,miR)-21재갑상선유두상암(PTC)、결절성갑상선종(NG)화암방정상갑상선조직(NTT)중적표체차이,탐토miRNA-21재갑상선유두상암중표체적의의.방법 수집자2011년4월지2012년8월우아원갑상선외과행갑상선수술적30례환자적갑상선유두상암조직급암방원단비류정상갑상선조직,기중남8례,녀22례,년령14 ～ 73세.결절성갑상선종15례.술중취재,립즉방입액담중보존후전입-70℃보존.채용실시정량역전록-취합매련반응(RT-qPCR)검측miR-21재갑상선암조직급비종류조직양본중적표체,병분석기여갑상선유두상암림상병리특정적상관성.결과 miR-21재갑상선유두상암중표체량명현고우비종류조직(포괄암방정상조직、결절성갑상선종)(P＜0.01),비종류조직간비교차이무통계학의의(P＞0.05).miR-21적표체여갑상선유두상암선외침윤명현상관(P＜0.05).결론 miR-21적표체재갑상선유두상암화비암조직중존재착명현차이,miR-21적표체여갑상선유두상암선외침윤유관.
Objective To study the expression difference of microRNA (miRNA)-21 in tumor tissue of papillary thyroid carcinoma (PTC),nodular goiter (NG) and cancer-adjacent normal thyroid tissue (NTT).Methods The surgically resected thyroid samples were collected in our hospital from April 2011 to August 2012,including 30 samples of PTC (8 males and 22 females,mean age of 45 years,ranging 14-73 years old),and paired cancer-adjacent NTC (N-PTC).In 15 cases of multinodular goiter (MNG),the surgically resected samples were collected and preserved at-70 °C in the liquid nitrogen immediately.The differences in the expression of miR-21 between PTC tissue and N-PTC were examined by using quantitative reverse transcriptase-polymerase chain reaction (RT-PCR),and the correlation between miR-21 expression and clinicopathologic features of PTC was analyzed.Results miR-21 in PTC tissue was significantly higher than in N-PTC and MNG (P ＜0.01).The expression of miR-21 in PTC was related with extrathyroidal invasion (P ＜ 0.05).Conclusion There are obvious differences in the miR-21 expression levels between PTC and N-PTC or MNG.The expression of miR-21 in PTC was related with extrathyroidal invasion.