中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
11期
2449-2451,封4
,共4页
米曰堂%李蕊%杨燕%范凤芝
米曰堂%李蕊%楊燕%範鳳芝
미왈당%리예%양연%범봉지
肝脏%冷缺血再灌注%模型,动物
肝髒%冷缺血再灌註%模型,動物
간장%랭결혈재관주%모형,동물
Liver%Cold ischemia/reperfusion%Model,animal
目的 建立一种门体转流下大鼠冷缺血再灌注损伤模型并与肝移植模型进行对比.方法 Wistar大鼠随机分为3组:A组(10只):对照组,B组(140只):肝移植组,C组(94只):脾脏移位肝脏冷缺血再灌注组.并进行门静脉压、肝功能及肝脏病理学变化检测.结果 B组和C组术后1d和7d均表现出特征性的缺血再灌注损伤表现.B组门静脉阻断后1 min和10 min门静脉压明显高于C组[1 min:(29.93±2.42) mm Hg比(22.91 ±4.01) mmHg,P＜0.01；10 min:(57.12±4.44) mm Hg比(18.32±4.42) mm Hg,P＜0.01).与A组比较,B组和C组术后即刻即出现胆红素(TBIL)、γ-谷氨酰转肽酶(GGT)和碱性磷酸酶(ALP)水平的明显升高(P＜0.05),B组和C组术后1d出现TBIL、GGT、谷丙转氨酶(ALT)和ALP水平的明显升高(P＜0.05),且B组改变明显高于C组(P＜0.05).术后7d,B组GGT仍明显高于A和C组[B组和A组:(2.16 ±0.34) IU/L比(1.39±0.36) IU/L,P＜0.05；B组和C组:(2.16±0.34) IU/L比(1.75±0.32) IU/L,P＜0.05].C组2周生存率高于B组(P＞0.05).结论 门体转流原位冷缺血再灌注损伤模型是理想的肝脏冷缺血再灌注损伤模型.
目的 建立一種門體轉流下大鼠冷缺血再灌註損傷模型併與肝移植模型進行對比.方法 Wistar大鼠隨機分為3組:A組(10隻):對照組,B組(140隻):肝移植組,C組(94隻):脾髒移位肝髒冷缺血再灌註組.併進行門靜脈壓、肝功能及肝髒病理學變化檢測.結果 B組和C組術後1d和7d均錶現齣特徵性的缺血再灌註損傷錶現.B組門靜脈阻斷後1 min和10 min門靜脈壓明顯高于C組[1 min:(29.93±2.42) mm Hg比(22.91 ±4.01) mmHg,P＜0.01；10 min:(57.12±4.44) mm Hg比(18.32±4.42) mm Hg,P＜0.01).與A組比較,B組和C組術後即刻即齣現膽紅素(TBIL)、γ-穀氨酰轉肽酶(GGT)和堿性燐痠酶(ALP)水平的明顯升高(P＜0.05),B組和C組術後1d齣現TBIL、GGT、穀丙轉氨酶(ALT)和ALP水平的明顯升高(P＜0.05),且B組改變明顯高于C組(P＜0.05).術後7d,B組GGT仍明顯高于A和C組[B組和A組:(2.16 ±0.34) IU/L比(1.39±0.36) IU/L,P＜0.05；B組和C組:(2.16±0.34) IU/L比(1.75±0.32) IU/L,P＜0.05].C組2週生存率高于B組(P＞0.05).結論 門體轉流原位冷缺血再灌註損傷模型是理想的肝髒冷缺血再灌註損傷模型.
목적 건립일충문체전류하대서랭결혈재관주손상모형병여간이식모형진행대비.방법 Wistar대서수궤분위3조:A조(10지):대조조,B조(140지):간이식조,C조(94지):비장이위간장랭결혈재관주조.병진행문정맥압、간공능급간장병이학변화검측.결과 B조화C조술후1d화7d균표현출특정성적결혈재관주손상표현.B조문정맥조단후1 min화10 min문정맥압명현고우C조[1 min:(29.93±2.42) mm Hg비(22.91 ±4.01) mmHg,P＜0.01；10 min:(57.12±4.44) mm Hg비(18.32±4.42) mm Hg,P＜0.01).여A조비교,B조화C조술후즉각즉출현담홍소(TBIL)、γ-곡안선전태매(GGT)화감성린산매(ALP)수평적명현승고(P＜0.05),B조화C조술후1d출현TBIL、GGT、곡병전안매(ALT)화ALP수평적명현승고(P＜0.05),차B조개변명현고우C조(P＜0.05).술후7d,B조GGT잉명현고우A화C조[B조화A조:(2.16 ±0.34) IU/L비(1.39±0.36) IU/L,P＜0.05；B조화C조:(2.16±0.34) IU/L비(1.75±0.32) IU/L,P＜0.05].C조2주생존솔고우B조(P＞0.05).결론 문체전류원위랭결혈재관주손상모형시이상적간장랭결혈재관주손상모형.
Objective To establish a rat model of in situ cold ischemia/reperfusion (I/R) with portosystemin shunt and compare to liver transplantation model.Methods Wistar rats were divided into three groups:group A (n =10),control group; group B (n =140),orthotopic liver transplantation group; and group C (n =94),liver cold ischemia group with splenic transposition (LCI).The parameters measured included portal venous pressure (PVP),liver enzymes [(total bilirubin (TBIL),alanine aminotransferase (ALT),alkaline phosphatase (ALP) and GGT),and 14-day survival rate.Results Both groups showed characteristic I/R histologic changes at 24th h and 7th day.PVP at 1 min and 10 min after porta hepatis clamping in group B was significantly higher than that in group C [1 min:(29.93 ± 2.42) mm Hg vs.(22.91 ±4.01) mmHg,P＜0.01; 10 min:(57.12 ±4.44) mmHgvs.(18.32 ±4.42) mmHg,P ＜0.01].TBIL,GGT and ALP were higher in groups B and C than in group A on the postoperative day (POD) 0 (P＜0.05).TBIL,GGT,ALT and ALP were higher in groups B and C than in group A on POD 1 (P ＜ 0.05),and the index was higer in Group B than group C on POD 1 (P ＜ 0.05).GGT was still higher in group B than in groups C and A on POD 7 [groups B and A:(2.16 ±0.34) IU/L vs.(1.39 ± 0.36) IU/L; groups B and C:(2.16 ±0.34) IU/L vs.(1.75 ±0.32) IU/L; P＜0.05 for both].The 14-day survival rate in group C was higher than in group A (P ＞ 0.05).Conclusion LCI model is ideal for the study on cold I/R injury.
