CAJ | 학술논문

目的观察凤尾草总黄酮对去势大鼠前列腺增生的治疗作用,并探讨其作用机制.方法皮下注射丙酸睾酮建立去势SD大鼠前列腺增生模型,造模成功后,随机分成6组,即空白组、模型组、凤尾草总黄酮组(高、中、低剂量组)、度他雄胺组和普适泰组,灌胃给予相应药物,空白组和模型组给予等体积蒸馏水.给药45 d后,观察各组大鼠前列腺湿重和指数,检测血清雄激素(T)、双氢睾酮(DHT)、雌二醇(E2)含量,光镜观察前列腺组织病理学改变,电镜观察前列腺细胞超微结构变化.结果凤尾草总黄酮能降低前列腺湿重及指数,各剂量组前列腺湿重[(1.750±0.245)、(1.998±0.328)、(2.064±0.196)g]及前列腺指数[(0.440±0.069)％、(0.481±0.085)％、(0.519±0.063)％]与模型组[(2.284±0.241)g、(0.586 ±0.100)％]比较均有降低,除凤尾草低剂量组的前列腺指数外,组间比较差异有统计学意义(P＜0.05),激素水平检测见凤尾草总黄酮高剂量组T、DHT[(0.18 ±0.01) μg/L、(16.3±1.61) ng/L]较模型组[(0.21 ±0.02) μg/L、(18.4±1.65) ng/L]降低,E2/T比值(7.24±0.63)较模型组(6.53±0.35)升高,组间差异有统计学意义(P＜0.05),凤尾草总黄酮还能够明显改善前列腺腺体的组织结构,电镜下可观察到用药后发生细胞凋亡现象.结论凤尾草总黄酮对丙酸睾酮诱导的去势大鼠前列腺增生的具有明显治疗作用,其作用机制可能与降低血清T和DHT水平,提高E2/T比值,使体内雌、雄激素维持在相对平衡状态并促进细胞凋亡有关.
목적 관찰봉미초총황동대거세대서전렬선증생적치료작용,병탐토기작용궤제.방법 피하주사병산고동건립거세SD대서전렬선증생모형,조모성공후,수궤분성6조,즉공백조、모형조、봉미초총황동조(고、중、저제량조)、도타웅알조화보괄태조,관위급여상응약물,공백조화모형조급여등체적증류수.급약45 d후,관찰각조대서전렬선습중화지수,검측혈청웅격소(T)、쌍경고동(DHT)、자이순(E2)함량,광경관찰전렬선조직병이학개변,전경관찰전렬선세포초미결구변화.결과 봉미초총황동능강저전렬선습중급지수,각제량조전렬선습중[(1.750±0.245)、(1.998±0.328)、(2.064±0.196)g]급전렬선지수[(0.440±0.069)％、(0.481±0.085)％、(0.519±0.063)％]여모형조[(2.284±0.241)g、(0.586 ±0.100)％]비교균유강저,제봉미초저제량조적전렬선지수외,조간비교차이유통계학의의(P＜0.05),격소수평검측견봉미초총황동고제량조T、DHT[(0.18 ±0.01) μg/L、(16.3±1.61) ng/L]교모형조[(0.21 ±0.02) μg/L、(18.4±1.65) ng/L]강저,E2/T비치(7.24±0.63)교모형조(6.53±0.35)승고,조간차이유통계학의의(P＜0.05),봉미초총황동환능구명현개선전렬선선체적조직결구,전경하가관찰도용약후발생세포조망현상.결론 봉미초총황동대병산고동유도적거세대서전렬선증생적구유명현치료작용,기작용궤제가능여강저혈청T화DHT수평,제고E2/T비치,사체내자、웅격소유지재상대평형상태병촉진세포조망유관.
Objective Research the intervention effect of total flavonoid of Pteris multifida in benign prostatic hyperplasia(BPH) castrated rats and the possible mechanism.Methods Removing of bilateral testes in SD rats,and subcutaneous injecting of testosterone propionate,established castrated rat model of benign prostatic hyperplasia.After the success of modeling,The model rats were randomly divided into 6 groups,the blank control group,model group,total flavonoid of Pteris multifida group (high,medium,low dose group),positive drug group (dutasteride group,prostat group),were fed with relevant drugs respectively,blank control group and model group were fed with the same volume of distilled water.Every group was given daily administration and consecutive for 45 days,then weighed the prostate and calculated the prostatic index (PI),measured the level of serum testosterone (T),dihyrotestotestosterone (DHT),estradiol (E2).Observe the pathological changes at the prostate tissue under the light microscope,observe the ultrastructural changes of prostate cells under the electron microscope.Results Total flavonoid of Pteris multifida can reduce the prostate wet weight and PI,Compared with rats in the BPH model group [(2.284 ±0.241) g,(0.586 ±0.100)％],total flavonoid of Pteris multifida treated groups had remarkably lower prostate wet weight [(1.750 ± 0.245),(1.998 0.328),(2.064 ± 0.196) g] and PI [(0.440 ± 0.069),(0.481 ± 0.085),(0.519 ± 0.063) ％],except the PI of low dose group,the difference between groups was statistically significant (P ＜ 0.05).The levels of T,E2 and DHT were determined via enzyme linked immunosorbent assay (ELISA).Compared with rats in the BPH model group [(0.21-±0.02) μg/L,(18.4 ± 1.65) ng/L),total flavonoid of Pteris multifida high dose treated group had remarkably lower T and DHT levels [(0.18 ±0.01) μg/L,(16.3 ± 1.61) ng/L],however and the ratio of E2/T in high dose group (7.24 ± 0.63) had increased compared with rats in the BPH model group (6.53 ± 0.35),the difference between groups was statistically significant (P ＜ 0.05).The pathological manifestations improved with various degrees.Observation with electron microscopic each dose group of total flavonoid of Pteris multifida showed low columnar epithelial cells,the nucleolus fiat,nucleus atrophy and apoptosis.Conclusion This study showed that subcutaneous injection of testosterone propionate could replicate of the model of BPH in rats successful.The total flavonoids of Pteris multifida could significantly reduce the prostate wet weight and PI.The mechanism might be total flavonoid of Pteris multifida could reduce T and DHT levels,increase E2/T ratio.maintain the estrogen and androgen in relative balance along with promote the apoptosis and regulate the cell cycle,so as to the treatment effect of inhibiting hyperplasia.