CAJ | 학술논문

目的探讨水溶性量子点硒化镉/硫化锌(CdSe/ZnS)对泌尿系肿瘤活细胞的毒性效应,观察其对泌尿系肿瘤细胞生长的影响.方法将不同浓度的量子点(5、10、20、40、80 nmol/L)分别作用于膀胱癌EJ细胞和前列腺癌PC-3细胞,通过细胞计数试剂盒(CCK-8)检测法来评价其对肿瘤细胞活性的影响.结果 CCK-8检测发现,两种活体细胞和量子点共同孵育24 h后,其存活率随着量子点浓度的升高均显示下降趋势,在量子点通常使用浓度(5、10、20 nmol/L)下,两种细胞的存活率分别为101.0％、99.2％、97.9％(EJ细胞)和100.1％、101.0％、99.0％(PC-3细胞),与对照组比较差异无统计学意义(P＞0.05),而在80 nmol/L高浓度时,两者存活率和对照组比较差异有统计学意义(P＜0.001),但是两种细胞的平均存活率也高达92.6％(EJ细胞)和95.8％(PC-3细胞).结论高浓度的量子点对膀胱癌EJ和前列腺癌PC-3细胞的活性有一定程度的影响,但是通常使用浓度的量子点对它们的存活率几乎没有影响.
목적 탐토수용성양자점서화력/류화자(CdSe/ZnS)대비뇨계종류활세포적독성효응,관찰기대비뇨계종류세포생장적영향.방법 장불동농도적양자점(5、10、20、40、80 nmol/L)분별작용우방광암EJ세포화전렬선암PC-3세포,통과세포계수시제합(CCK-8)검측법래평개기대종류세포활성적영향.결과 CCK-8검측발현,량충활체세포화양자점공동부육24 h후,기존활솔수착양자점농도적승고균현시하강추세,재양자점통상사용농도(5、10、20 nmol/L)하,량충세포적존활솔분별위101.0％、99.2％、97.9％(EJ세포)화100.1％、101.0％、99.0％(PC-3세포),여대조조비교차이무통계학의의(P＞0.05),이재80 nmol/L고농도시,량자존활솔화대조조비교차이유통계학의의(P＜0.001),단시량충세포적평균존활솔야고체92.6％(EJ세포)화95.8％(PC-3세포).결론 고농도적양자점대방광암EJ화전렬선암PC-3세포적활성유일정정도적영향,단시통상사용농도적양자점대타문적존활솔궤호몰유영향.
Objective To detect the cytotoxic effect of quantum dots(CdSe/ZnS QDs) on the urinary tract tumor cells,Study the influence of CdSe/ZnS quantum dots on growth of the urinary tract tumor cells.Methods The cytotoxic effect of QDs in different concerntrations (5,10,20,40,80 nmol/L) was detected on bladder cancer cell line EJ and prostate cancer cell line PC-3 by cell counting kit-8 (CCK-8),assessing the activities of tumor cells.Results Two kinds of live cells incubated with QDs for 24 hours,using CCK-8 test,performed that the survival rate of cells was decreased with the increasing of QDs concentrations.However,in the common concentration (5,10 and 20 nmol/L),the survival rate of the two kinds of cells was 101％,99.2％,97.9％ (EJ cell line)and 100.1％,101.0％,99.0％ (PC-3 cell line) respectively.Moreover,no statistical difference in survival rate was observed between experimental and control groups (P ＞ 0.05) ; in the high concentration (80 nmol/L),there were obvious difference between experimental and control groups (P ＜ 0.01),however,the survival rates of bladder cancer cell line EJ and prostate cancer cell line PC-3 could still remained 92.6％ and 95.8％,respectively.Conclusion The quantum dots in high concentrations have a certain degree of influence on the activity of bladder cancer cell line EJ and prostate cancer cell line PC-3,however,for the conventional use,the concentration of quantum dots have little impact on their survival.