中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
12期
2511-2513
,共3页
杨森%南存金%木海琦%王怡君%陈映鹤
楊森%南存金%木海琦%王怡君%陳映鶴
양삼%남존금%목해기%왕이군%진영학
膀胱癌%术后复发%p53%K-ras%基因突变
膀胱癌%術後複髮%p53%K-ras%基因突變
방광암%술후복발%p53%K-ras%기인돌변
Bladder cancer%Postoperative recurrence%p53%K-ras%Gene mutation
目的 探讨p53和K-ras基因突变与表浅性膀胱癌术后复发和生存率的关系.方法 选取表浅性膀胱癌切除术的137例患者,并选取29例正常膀胱黏膜组织作为对照,分别提取组织的DNA,采用聚合酶链反应-单链构象多态性分析(PCR-SSCP)和直接测序法分别分析p53和K-ras基因突变,并探讨p53和K-ras突变与表浅性膀胱癌术后复发及生存率的关系.结果 p53突变主要集中于外显子7和8,K-ras集中于外显子1和2.正常膀胱黏膜组织中p53和K-ras基因单突变率分别为3.45％(1/29)和6.90％ (2/29),p53和K-ras基因双突变率为0％ (0/29).膀胱癌组织中p53和K-ras单突变的发生率分别为43.07％ (59/137)和30.66％ (42/137),p53和K-ras双突变的发生率为26.28％(36/137).膀胱癌组织中p53和K-ras突变的发生率明显高于正常膀胱黏膜的发生率,差异有统计学意义(P＜0.05).p53和K-ras双突变的术后复发率(75.00％)明显高于p53和K-ras单突变术后复发率(30.51％和28.57％),差异有统计学意义(P＜0.05).p53和K-ras双突变的3年生存率明显低于p53和K-ras单突变的3年生存率,差异有统计学意义(P＜0.05).结论 p53和K-ras突变可能是导致膀胱癌术后复发和生存率较低的原因之一.
目的 探討p53和K-ras基因突變與錶淺性膀胱癌術後複髮和生存率的關繫.方法 選取錶淺性膀胱癌切除術的137例患者,併選取29例正常膀胱黏膜組織作為對照,分彆提取組織的DNA,採用聚閤酶鏈反應-單鏈構象多態性分析(PCR-SSCP)和直接測序法分彆分析p53和K-ras基因突變,併探討p53和K-ras突變與錶淺性膀胱癌術後複髮及生存率的關繫.結果 p53突變主要集中于外顯子7和8,K-ras集中于外顯子1和2.正常膀胱黏膜組織中p53和K-ras基因單突變率分彆為3.45％(1/29)和6.90％ (2/29),p53和K-ras基因雙突變率為0％ (0/29).膀胱癌組織中p53和K-ras單突變的髮生率分彆為43.07％ (59/137)和30.66％ (42/137),p53和K-ras雙突變的髮生率為26.28％(36/137).膀胱癌組織中p53和K-ras突變的髮生率明顯高于正常膀胱黏膜的髮生率,差異有統計學意義(P＜0.05).p53和K-ras雙突變的術後複髮率(75.00％)明顯高于p53和K-ras單突變術後複髮率(30.51％和28.57％),差異有統計學意義(P＜0.05).p53和K-ras雙突變的3年生存率明顯低于p53和K-ras單突變的3年生存率,差異有統計學意義(P＜0.05).結論 p53和K-ras突變可能是導緻膀胱癌術後複髮和生存率較低的原因之一.
목적 탐토p53화K-ras기인돌변여표천성방광암술후복발화생존솔적관계.방법 선취표천성방광암절제술적137례환자,병선취29례정상방광점막조직작위대조,분별제취조직적DNA,채용취합매련반응-단련구상다태성분석(PCR-SSCP)화직접측서법분별분석p53화K-ras기인돌변,병탐토p53화K-ras돌변여표천성방광암술후복발급생존솔적관계.결과 p53돌변주요집중우외현자7화8,K-ras집중우외현자1화2.정상방광점막조직중p53화K-ras기인단돌변솔분별위3.45％(1/29)화6.90％ (2/29),p53화K-ras기인쌍돌변솔위0％ (0/29).방광암조직중p53화K-ras단돌변적발생솔분별위43.07％ (59/137)화30.66％ (42/137),p53화K-ras쌍돌변적발생솔위26.28％(36/137).방광암조직중p53화K-ras돌변적발생솔명현고우정상방광점막적발생솔,차이유통계학의의(P＜0.05).p53화K-ras쌍돌변적술후복발솔(75.00％)명현고우p53화K-ras단돌변술후복발솔(30.51％화28.57％),차이유통계학의의(P＜0.05).p53화K-ras쌍돌변적3년생존솔명현저우p53화K-ras단돌변적3년생존솔,차이유통계학의의(P＜0.05).결론 p53화K-ras돌변가능시도치방광암술후복발화생존솔교저적원인지일.
Objective To explore the relationship of K-ras and p53 gene mutation and superficial bladder cancer postoperative recurrence and survival rate.Methods 137 bladder cancer patients subject to surgery were selected from December 2007 to June 2012.Twenty-nine normal tissue samples were selected as controls.Total DNA was extracted form bladder cancer tissues and normal tissues.p53 and K-ras gene mutations were analyzed by PCR-SSCP and gene sequencing.The relationship between p53 and K-ras gene mutation and superficial bladder cancer postoperative recurrence and survival rate was analyzed.Results p53 gene mutation was found in exons 7 and 8,and K-ras gene mutation in exons 1 and 2.p53 and K-ras gene single mutation rate in normal tissues was 3.45％ and 6.90％ respectively.The double mutation rate of p53 and K-ras gene in normal tissues was 0.The mutation rate of p53 and K-ras in bladder cancer tissues was 43.07％ (59/137) and 30.66％ (42/137) respectively.The double mutation rate of p53 and K-ras in bladder cancer tissues was 32.14％.The double mutation rate of p53 and K-ras in bladder cancer tissue was significantly higher than that in normal tissue (P ＜ 0.05).Postoperative recurrence in double mutation of p53 and K-ras was significantly higher than that in single mutation of p53 and K-ras (P ＜ 0.05).The 3-year survival rate in double mutation of p53 and K-ras was significantly higher than that in single mutation of p53 and K-ras (P ＜ 0.05).Conclusion p53 and K-ras gene mutation is one of the reasons leading to high postoperative recurrence and lower survival rate.p53 and K-ras gene mutation can be used as a clinical prognostic indicators.
