CAJ | 학술논문

目的探讨长链非编码RNA(LncRNA)在人类膀胱尿路上皮癌组织中的表达差异及意义.方法收集手术切除12例尿路上皮癌及3例正常人膀胱尿路上皮黏膜,提取RNA,逆转录成cDNA并标记,在标准条件下将标记好的与芯片进行杂交,使用荧光扫描仪扫描荧光强度并分析基因表达谱差异.结果采用Enhancer LncRNA profiling和Rinn LncRNAs profiling两种分析方法,分别发现了7条和25条差异表达的基因,并通过对其中3条差异表达基因进行逆转录-聚合酶链反应(RT-PCR)检测证实其表达与芯片结果一致.结论 LncRNA与膀胱尿路上皮癌发生、发展有着密切联系.
목적 탐토장련비편마RNA(LncRNA)재인류방광뇨로상피암조직중적표체차이급의의.방법 수집수술절제12례뇨로상피암급3례정상인방광뇨로상피점막,제취RNA,역전록성cDNA병표기,재표준조건하장표기호적여심편진행잡교,사용형광소묘의소묘형광강도병분석기인표체보차이.결과 채용Enhancer LncRNA profiling화Rinn LncRNAs profiling량충분석방법,분별발현료7조화25조차이표체적기인,병통과대기중3조차이표체기인진행역전록-취합매련반응(RT-PCR)검측증실기표체여심편결과일치.결론 LncRNA여방광뇨로상피암발생、발전유착밀절련계.
Objective To compare the gene expression differences between human urothelial carcinoma and normal bladder tissues,and the significances in utothelial carcinoma of bladder.Methods We collected 12 specimens of urothelial carcinoma and 3 specimens of normal bladder mucosa,then we extracted RNA,transcribe it into cDNA and labeled it.Then we used the probe which have already be labeled hybridize with microarray.After washing,slides were scanned with the Agilent DNA Microarray Scanner.Data was extracted using Agilent Feature Extraction software.Results Compared with nomarl bladder tissues,we found 7 differentially expressed genes by Enhancer long noncoding RNA (LncRNA) profiling and 25 differentially expressed genes by Rinn LncRNAs profiling.Then we used reverse transcriptase-polymerase chain reaction (RT-PCR) assay to amplify three of the differentially expressed genes,its expression consistent with the microarray results.Conclusion LncRNA have close relationship with urothelial carcinoma' s developing.It may be an significant tumor marker for urothelial carcinoma of bladder,which can help us make early diagnosis,prediction and open new paths of gene therapy for bladder urothelial carcinoma.