CAJ | 학술논문

目的观察糖尿病性膀胱病(DCP)豚鼠膀胱Cajal间质细胞(Icc)数量、形态和结构的改变,探讨ICC在DCP发病机制中的作用.方法 50只雌性豚鼠,随机选择42只作为实验组,8只作为对照组(C组).以单次腹腔注射链脲佐菌素法诱导实验组糖尿病豚鼠模型.尿动力学检查确定膀胱功能失代偿豚鼠(A组,即DCP组)及代偿豚鼠(B组,即代偿组).免疫组织化学荧光染色、激光共聚焦显微镜、透射电镜技术检测膀胱ICC形态、数量与结构变化.结果 A组膀胱ICC面积与膀胱组织面积百分比[(2.18±0.04)％]较C组[(2.81±0.04)％]及B组[(2.80±0.05)％]显著降低(P＜0.01).DCP组膀胱ICC数量减少,细胞形态及超微结构发生明显改变.结论糖尿病膀胱病豚鼠膀胱ICC数量减少、形态结构改变,提示ICC可能在DCP的发病过程中起重要作用.
목적 관찰당뇨병성방광병(DCP)돈서방광Cajal간질세포(Icc)수량、형태화결구적개변,탐토ICC재DCP발병궤제중적작용.방법 50지자성돈서,수궤선택42지작위실험조,8지작위대조조(C조).이단차복강주사련뇨좌균소법유도실험조당뇨병돈서모형.뇨동역학검사학정방광공능실대상돈서(A조,즉DCP조)급대상돈서(B조,즉대상조).면역조직화학형광염색、격광공취초현미경、투사전경기술검측방광ICC형태、수량여결구변화.결과 A조방광ICC면적여방광조직면적백분비[(2.18±0.04)％]교C조[(2.81±0.04)％]급B조[(2.80±0.05)％]현저강저(P＜0.01).DCP조방광ICC수량감소,세포형태급초미결구발생명현개변.결론 당뇨병방광병돈서방광ICC수량감소、형태결구개변,제시ICC가능재DCP적발병과정중기중요작용.
Objective To investigate the bladder interstitial cells of Cajal (ICC) function in the pathogenesis of the diabetic cystopathy.Methods Fifty short hair Britain female guinea pigs were divided into two groups randomly,42 as the experiment group and the other 8 as the control group (group C).The experiment group was intraperitoneally injected with streptozotocin (STZ) to establish diabetic models.Diabetic guinea pigs were further divided into the decompensated bladder function group (namely DCP,group A) and compensated group (group B) by urodynamic study.Immunofluorescence staining,confocal laser microscope and transmission electron microscopy (TEM) technology were used to detect the morphology and ultrastructure changes of the ICC.Results The percentage of the ICC in group A was significantly lower than that of the group B and group C [(2.18 ± 0.04) ％ vs.(2.80 ± 0.05,2.81 ± 0.04) ％ respectively,P ＜0.01].Under the electron microscopic observation,the mitochondria in the ICC were reduced in the DCP group.The substrate also became less and shallow,and crest got shorter or even disappeared.Conclusion ICC may play an important role in the development of the DCP.