CAJ | 학술논문

目的探讨转染Adv-IκB激酶2的显性负性突变体(IKK2dn)基因并负载供者抗原的受者树突状细胞(DC)诱导产生的CD4+ CD25-T细胞回输是否延长同种异体肾移植大鼠的存活时间,并探讨其机制.方法获取和培养受者Lewis大鼠骨髓源性DC,转染IKK2dn基因并负载BN大鼠可溶性抗原,与BN大鼠的T细胞初次混合淋巴细胞反应(MLR),72 h后采用免疫磁珠分选法筛选CD4+ CD25-T细胞.肾移植受者为Lewis大鼠,分为初始T细胞组、Adv0-CD4+T细胞组、CD4+CD25-T细胞组、排斥组,术前7d分别输注1×107个初始CD4+T细胞、Adv-0-DC诱导产生的CD4+ CD25-T细胞、Adv-IKK2dn-DC诱导产生的CD4+ CD25-T细胞和等量生理盐水,供者均为BN大鼠.另设第三方供者组,术前处理同治疗组,供者为Wistar大鼠.移植术后观察各组大鼠存活时间和发生排斥反应;测定受者T淋巴细胞增殖能力;检测血清白细胞介素(IL)-2、IL-10、γ-干扰素(IFN-γ)以及转化生长因子-β(TGF-β)表达水平;监测血肌酐(Cr)水平;病理学检查评定排斥级别.结果 CD4+ CD25-T细胞组肾移植大鼠生存时间为(28.50 ±2.36)d,较其他各组显著延长(P＜0.01);与其他各组比较,CD4+ CD25-T细胞组表达高水平IL-10和TGF-β(P＜0.05);与排斥组、Adv0-CD4+T细胞组、第三方供者组比较,CD4+ CD25-T细胞组低表达IL-2和IFN-γ(P＜0.05);CD4+ CD25-T细胞组T淋巴细胞的增殖能力明显低于其他4组(P＜0.05);病理学检查CD4+CD25-T细胞组发生排斥反应的病理级别较低.结论转染IKK2dn基因并负载供者抗原的受者DC诱导产生的CD4+ CD25-T细胞回输可以延长同种异体肾移植大鼠生存时间,并具有针对供者的特异性,其机制可能与诱导的IL-10、TGF-β的高分泌有关.
목적 탐토전염Adv-IκB격매2적현성부성돌변체(IKK2dn)기인병부재공자항원적수자수돌상세포(DC)유도산생적CD4+ CD25-T세포회수시부연장동충이체신이식대서적존활시간,병탐토기궤제.방법 획취화배양수자Lewis대서골수원성DC,전염IKK2dn기인병부재BN대서가용성항원,여BN대서적T세포초차혼합림파세포반응(MLR),72 h후채용면역자주분선법사선CD4+ CD25-T세포.신이식수자위Lewis대서,분위초시T세포조、Adv0-CD4+T세포조、CD4+CD25-T세포조、배척조,술전7d분별수주1×107개초시CD4+T세포、Adv-0-DC유도산생적CD4+ CD25-T세포、Adv-IKK2dn-DC유도산생적CD4+ CD25-T세포화등량생리염수,공자균위BN대서.령설제삼방공자조,술전처리동치료조,공자위Wistar대서.이식술후관찰각조대서존활시간화발생배척반응;측정수자T림파세포증식능력;검측혈청백세포개소(IL)-2、IL-10、γ-간우소(IFN-γ)이급전화생장인자-β(TGF-β)표체수평;감측혈기항(Cr)수평;병이학검사평정배척급별.결과 CD4+ CD25-T세포조신이식대서생존시간위(28.50 ±2.36)d,교기타각조현저연장(P＜0.01);여기타각조비교,CD4+ CD25-T세포조표체고수평IL-10화TGF-β(P＜0.05);여배척조、Adv0-CD4+T세포조、제삼방공자조비교,CD4+ CD25-T세포조저표체IL-2화IFN-γ(P＜0.05);CD4+ CD25-T세포조T림파세포적증식능력명현저우기타4조(P＜0.05);병이학검사CD4+CD25-T세포조발생배척반응적병리급별교저.결론 전염IKK2dn기인병부재공자항원적수자DC유도산생적CD4+ CD25-T세포회수가이연장동충이체신이식대서생존시간,병구유침대공자적특이성,기궤제가능여유도적IL-10、TGF-β적고분비유관.
Objective To observe whether CD4 + CD25-T cells induced by recipient-derived immature dendritic cells (imDC) transfected with dominant negative form of IκB kinases 2 (IKK2dn) gene and loaded with donor antigen can prolong the survival time of kidney transplantation in rats and investigate its probably.Methods DC were cultured from recipient rats (Lewis) bone marrow,transfected with AdvIKK2dn and loaded with donor antigen,then cultured with Lewis rats lymphocytes in primary mixed lymphocyte reaction (MLR).The CD4 + CD25-T cells were separated by immune magnetic beads method after 72 hours.Male Brown Norway rats and Lewis rats were used as donors and recipients respectively,Four groups were set up(naive T cells group,Adv0-CD4 + T cells group,CD4 + CD25-T cells group and rejection group),receiving 1 × 107 naive CD4 + T-cell,Adv-0-DC-generated CD4 + CD25-T-cell,Adv-IKK2dnDC-generated CD4 + CD25 T-cell and equal volume of normal saline,respectively 7 days before transplantation.In the third party donor group,wistar rats as donors were treated the same as CD4 + CD25 T cells group before transplantation.After transplantation,the survival time of recipients were observed; the T lymphocyte proliferation in recipients was measured; the levels of serum interleukin (IL)-2,IL-10,interferon-γ(IFN-γ),transforming growth factor-β (TGF-β) were detected; serum creatinine levels were monitored ; pathological changes were examined to identify the grade of rejection,Results Compared with other groups,CD4 + CD25-T cells group markedly prolonged the survival time of renal allografts [(28.50 ± 2.36) d,P ＜0.01],and had higher expression of IL-10 and TGF-β (P ＜0.05).Compared with Adv0CD4 + T cells group,rejection group,the third party donor group,expression levels of IL-2 and IFN-γwere lower(P ＜0.05).In contrast to other groups,CD4 + CD25-T cells group elicited markedly lower proliferative responses (P ＜ 0.05) and lower grade as estimated by pathological examination.Conclusion These findings suggested that CD4 + CD25 T cells induced by recipient-derived immature DC transfected by IKK2dn and loaded with donor antigen could prolong renal allograft survival in a donor-specific manner,and it maybe by expressing high levels of IL-10 and TGF-β.