CAJ | 학술논문

目的利用水溶性ZnSe:Mn/ZnSe掺杂量子点优良的光学特性实现对肝癌细胞株HCCLM9甲胎蛋白(AFP)抗原的特异性免疫荧光标记成像.方法用巯基丙酸修饰的ZnSe∶Mn/ZnSe掺杂量子点,制备水溶性ZnSe∶Mn/ZnSe-IgG复合物探针.荧光、紫外光光谱分析及透射电镜研究其特性.通过间接免疫荧光法,用水溶性ZnSe∶Mn/ZnSe-IgG复合物探针标记肝癌细胞株HCCLM9 AFP抗原.噻唑蓝(MTT)法检测该复合物探针的细胞毒性,并同传统量子点探针比较.双光子共聚焦激光显微镜观察成像,并进行连续激发实验,同传统量子点探针及有机荧光探针比较光稳定性.结果该水溶性ZnSe∶Mn/ZnSe-IgG复合物探针具有激发光谱宽、荧光强度高的特点,生物相容性较传统量子点好,无明显细胞毒性.用该探针对肝癌细胞成像清晰,其荧光在共聚焦激光连续激发情况下无明显衰减.结论 ZnSe∶Mn/ZnSe掺杂量子点是一种较理想的荧光材料,比传统量子点毒性更小,并且比传统的有机荧光染料具有更优良的光学特性.
목적 이용수용성ZnSe:Mn/ZnSe참잡양자점우량적광학특성실현대간암세포주HCCLM9갑태단백(AFP)항원적특이성면역형광표기성상.방법 용구기병산수식적ZnSe∶Mn/ZnSe참잡양자점,제비수용성ZnSe∶Mn/ZnSe-IgG복합물탐침.형광、자외광광보분석급투사전경연구기특성.통과간접면역형광법,용수용성ZnSe∶Mn/ZnSe-IgG복합물탐침표기간암세포주HCCLM9 AFP항원.새서람(MTT)법검측해복합물탐침적세포독성,병동전통양자점탐침비교.쌍광자공취초격광현미경관찰성상,병진행련속격발실험,동전통양자점탐침급유궤형광탐침비교광은정성.결과 해수용성ZnSe∶Mn/ZnSe-IgG복합물탐침구유격발광보관、형광강도고적특점,생물상용성교전통양자점호,무명현세포독성.용해탐침대간암세포성상청석,기형광재공취초격광련속격발정황하무명현쇠감.결론 ZnSe∶Mn/ZnSe참잡양자점시일충교이상적형광재료,비전통양자점독성경소,병차비전통적유궤형광염료구유경우량적광학특성.
Objective Special immune fluorescent imaging of human hepatocellular carcinoma cell line HCCLM9 AFP antigen with water soluble ZnSe:Mn/ZnSe doped-quantum dots modified by 3-Mercaptopropionic acid.Methods ZnSe:Mn/ZnSe doped-quantum dots were modified with 3-Mercaptopropionic acid to prepare water soluble ZnSe:Mn/ZnSe-IgG probes.The characters of these probes were studied through spectra analyses and transmission electron microscope.These probes were used for special immune fluorescent imaging of human hepatocellular carcinoma cell line HCCLM9 alpha fetal protein (AFP) antigen.Methyl thiazol tetrazolium (MTT) assay were used to compare the cytotoxicity between ZnSe:Mn/ ZnSe-IgG and CdSe/ZnSe-IgG probes.Images were obtained through two-photon confocal laser microscope.Photostability of ZnSe:Mn/ZnSe-IgG probes were studied under continuous illumination by a high intensity laser,comparing with the CdSe/ZnSe and organic fluorescence probes.Results These water soluble ZnSe:Mn/ZnSe-IgG probes have good properties such as broad excite spectra,high fluorescence intensity.They have lower cytotoxicity than CdSe/ZnSe probes.They can specifically and effectively recognize tumor markers.The labeling signals of ZnSe:Mn/ZnSe doped-quantum dots were found to be specific and bright.They exhibited exceptional photostability during continuous illumination for long time by a high intensity laser.Conclusion ZnSe:Mn/ZnSe doped-quantum dots are a kind of ideal fluorescent material with spectral characters and photostability better than conventional organic dye,and they also have better biocompatibility than traditional quantum dots,which can be well used in biomedical imaging area.