CAJ | 학술논문

目的观察大黄素在体外对胆管癌QBC939细胞增殖的抑制作用及信号转导通路丝裂原活化蛋白激酶(MAPK)的影响.方法用大黄素作为干预因素,剂量效应组分别用不同浓度大黄素的培养液与QBC939细胞共培养;检测细胞增殖、凋亡,逆转录-聚合酶链反应(RT-PCR)检测细胞中c-myc mRNA表达,Western blot检测细胞中c-myc、MAPK、p-MAPK蛋白质的表达.结果大黄素抑制QBC939细胞增殖,0、20、40、80 μmol/L大黄素对QBC939细胞增殖抑制率分别为(4.73±0.88)％、(21.27±4.04)％、(38.68±4.57)％、(62.45±6.12)％ (P ＜0.05),c-myc、p-MAPK蛋白明显下降,MAPK蛋白表达无变化.结论大黄素可能通过抑制MAPK信号转导途径抑制QBC939细胞增殖.
목적 관찰대황소재체외대담관암QBC939세포증식적억제작용급신호전도통로사렬원활화단백격매(MAPK)적영향.방법 용대황소작위간예인소,제량효응조분별용불동농도대황소적배양액여QBC939세포공배양;검측세포증식、조망,역전록-취합매련반응(RT-PCR)검측세포중c-myc mRNA표체,Western blot검측세포중c-myc、MAPK、p-MAPK단백질적표체.결과 대황소억제QBC939세포증식,0、20、40、80 μmol/L대황소대QBC939세포증식억제솔분별위(4.73±0.88)％、(21.27±4.04)％、(38.68±4.57)％、(62.45±6.12)％ (P ＜0.05),c-myc、p-MAPK단백명현하강,MAPK단백표체무변화.결론 대황소가능통과억제MAPK신호전도도경억제QBC939세포증식.
Objective To investigate the effects of Emodin in growing inhibition and down-regulating mitogen-activated protein kinase (MAPK) in vitro.Methods QBC939 cells were cultured with Emodin for different concentrations of Emodin.The inhibition of cell proliferation was detected by methyl thiazol tetrazolium (MTT).The expressions of c-myc mRNA and protein were detected by reverse transcriptasepolymerase chain reaction (RT-PCR) and Western blotting,MAPK,p-MAPK was detected by Western blotting.Results Emodin inhibited cell proliferation of QBC939 cell.apoptosis rate of QBC939 cells treated with 0,20,40,80 μmol/L Emodin for48 h was (4.73 ±0.88)％,(21.27 ±4.04)％,(38.68 ±4.57) ％,(62.45 ± 6.12) ％ (P ＜ 0.05) Emodin could down-regulating c-myc,p-MAPK level (P ＜0.05).but MAPK expression level had no significant change.Conclusion Emodin can inhibite cell proliferation of human cholangiocarcinoma cell QBC939.The mechanism is associated with down-regulating MAPK.