中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
12期
2593-2595
,共3页
范钰%徐娟%满昌峰%彭辉勇%单秀红%沈荣%祁卫东
範鈺%徐娟%滿昌峰%彭輝勇%單秀紅%瀋榮%祁衛東
범옥%서연%만창봉%팽휘용%단수홍%침영%기위동
大肠肿瘤%超顺%纳米%榄香烯%增殖%侵袭%微小RNA-155
大腸腫瘤%超順%納米%欖香烯%增殖%侵襲%微小RNA-155
대장종류%초순%납미%람향희%증식%침습%미소RNA-155
Colorectal carcinoma%Superparamagnetism%Nanometre%Elemene%Proliferation%Invasion%MicroRNA-155
目的 观察超顺磁性纳米榄香烯对人大肠癌细胞增殖和侵袭的影响,并探讨其可能机制.方法 制备超顺磁性纳米榄香烯,采用不同浓度的超顺磁性纳米榄香烯处理人大肠癌HCT116细胞后,以噻唑蓝(MTT)法检测癌细胞增殖,以Transwell方法检测癌细胞侵袭能力,采用实时荧光定量聚合酶链反应(FQ-PCR)方法检测癌细胞微小RNA(miR)-155 mRNA水平.结果 超顺磁性纳米榄香烯对人大肠癌HCT116细胞增殖具有一定的抑制作用.在2.5～25.0 mg/L浓度范围内,超顺磁性纳米榄香烯对癌细胞的抑制与浓度呈正相关(r =0.685);Transewell结果显示,空白对照组、不同浓度(5、10、20 mg/L)超顺磁性纳米榄香烯处理组细胞穿过滤膜的细胞分别为(44.8±1.5)、(32.2±1.3)、(18.2±1.5)和(8.6±1.2)个.FQ-PCR结果显示,与空白对照组比较,超顺磁性纳米榄香烯组miR-155 mRNA水平明显下调,且呈浓度依赖性(P＜0.01).结论 超顺磁性纳米榄香烯可降低人大肠癌细胞增殖、侵袭能力,下调miR-155是其重要机制之一.
目的 觀察超順磁性納米欖香烯對人大腸癌細胞增殖和侵襲的影響,併探討其可能機製.方法 製備超順磁性納米欖香烯,採用不同濃度的超順磁性納米欖香烯處理人大腸癌HCT116細胞後,以噻唑藍(MTT)法檢測癌細胞增殖,以Transwell方法檢測癌細胞侵襲能力,採用實時熒光定量聚閤酶鏈反應(FQ-PCR)方法檢測癌細胞微小RNA(miR)-155 mRNA水平.結果 超順磁性納米欖香烯對人大腸癌HCT116細胞增殖具有一定的抑製作用.在2.5～25.0 mg/L濃度範圍內,超順磁性納米欖香烯對癌細胞的抑製與濃度呈正相關(r =0.685);Transewell結果顯示,空白對照組、不同濃度(5、10、20 mg/L)超順磁性納米欖香烯處理組細胞穿過濾膜的細胞分彆為(44.8±1.5)、(32.2±1.3)、(18.2±1.5)和(8.6±1.2)箇.FQ-PCR結果顯示,與空白對照組比較,超順磁性納米欖香烯組miR-155 mRNA水平明顯下調,且呈濃度依賴性(P＜0.01).結論 超順磁性納米欖香烯可降低人大腸癌細胞增殖、侵襲能力,下調miR-155是其重要機製之一.
목적 관찰초순자성납미람향희대인대장암세포증식화침습적영향,병탐토기가능궤제.방법 제비초순자성납미람향희,채용불동농도적초순자성납미람향희처리인대장암HCT116세포후,이새서람(MTT)법검측암세포증식,이Transwell방법검측암세포침습능력,채용실시형광정량취합매련반응(FQ-PCR)방법검측암세포미소RNA(miR)-155 mRNA수평.결과 초순자성납미람향희대인대장암HCT116세포증식구유일정적억제작용.재2.5～25.0 mg/L농도범위내,초순자성납미람향희대암세포적억제여농도정정상관(r =0.685);Transewell결과현시,공백대조조、불동농도(5、10、20 mg/L)초순자성납미람향희처리조세포천과려막적세포분별위(44.8±1.5)、(32.2±1.3)、(18.2±1.5)화(8.6±1.2)개.FQ-PCR결과현시,여공백대조조비교,초순자성납미람향희조miR-155 mRNA수평명현하조,차정농도의뢰성(P＜0.01).결론 초순자성납미람향희가강저인대장암세포증식、침습능력,하조miR-155시기중요궤제지일.
Objective To study the effects and mechanism of elemene on proliferation and invasion of human colorectal cancer cell.Methods Superparamagnetic nano elemene was prepared.After human colorectal cancer HCT116 cell were treated with different doses of superparamagnetic nano elemene,and proliferation of cancer cell was studied by methyl thiazol tetrazolium (MTT) assay,and invasion ability were determined by transwell assay,and the microRNA (miR)-155 mRNA were detected by Real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) assay.Results Superparamagnetic nano elemene has an inhibitory effect on the proliferation ofhuman colorectal cancer HCT116 cell line.In the concentration range from 2.5 mg/L to 25.0 mg/L,the inhibition was positively correlated with concentration of magnetic nano elemene on cancer cells (r =0.685) ; Transewell results showed that,was superparamagnetic the cells treated cells through membrane filter was 44.8 ± 1.5,32.2 ± 1.3,18.2 ± 1.5 and 8.6 ± 1.2,respectively,in the blank control group,groups of different concentrations (5,10,20 mg/L) nanometer magnetic element.FQ-PCR results showed that,compared with the blank control group,miR-155 mRNA level of superparamagnetic nano elemene group were significantly reduced,in a dose-dependent manner (P ＜ 0.01).Conclusion Superparamagnetic nano elemene can reduce cell proliferation and invasion of colorectal cancer through down-regulating of miR-155.
