中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
12期
2606-2608
,共3页
李中林%梅鹏金%白津%时梅林%李连涛%范月超%郑骏年
李中林%梅鵬金%白津%時梅林%李連濤%範月超%鄭駿年
리중림%매붕금%백진%시매림%리련도%범월초%정준년
乳腺癌转移抑制基因1%胶质瘤%黏附%迁移
乳腺癌轉移抑製基因1%膠質瘤%黏附%遷移
유선암전이억제기인1%효질류%점부%천이
Breast cancer metastasis suppressor 1%Gioma%Adhesion%Migration
目的 探讨乳腺癌转移抑制基因1(BRMS1)在胶质瘤细胞黏附和迁移中的作用和机制.方法 脂质体介导真核表达质粒pEGFP-BRMS1和对照质粒pEGFP-N2转入人胶质瘤U251和U87细胞中,Western blot技术检测BRMS1蛋白的表达.黏附实验和迁移实验检测细胞黏附和迁移能力.Western blot检测磷酸化黏着斑激酶(FAK) (p-FAK)和磷酸化酪氨酸激酶(Src)(p-Src)蛋白的表达.结果 与对照组比较,转染pEGFP-BRMS1质粒24 h后U251和U87细胞中BRMS1蛋白表达显著增高;细胞黏附能力分别下降60％和65％;细胞穿越Transwell小室能力分别下降64％和68％,差异均有统计学意义(P＜0.01);转染BRMS1后两种细胞p-FAK和p-Src表达量明显减少.结论 BRMS1通过减少p-FAK/p-Src表达抑制胶质瘤细胞黏附和迁移能力.
目的 探討乳腺癌轉移抑製基因1(BRMS1)在膠質瘤細胞黏附和遷移中的作用和機製.方法 脂質體介導真覈錶達質粒pEGFP-BRMS1和對照質粒pEGFP-N2轉入人膠質瘤U251和U87細胞中,Western blot技術檢測BRMS1蛋白的錶達.黏附實驗和遷移實驗檢測細胞黏附和遷移能力.Western blot檢測燐痠化黏著斑激酶(FAK) (p-FAK)和燐痠化酪氨痠激酶(Src)(p-Src)蛋白的錶達.結果 與對照組比較,轉染pEGFP-BRMS1質粒24 h後U251和U87細胞中BRMS1蛋白錶達顯著增高;細胞黏附能力分彆下降60％和65％;細胞穿越Transwell小室能力分彆下降64％和68％,差異均有統計學意義(P＜0.01);轉染BRMS1後兩種細胞p-FAK和p-Src錶達量明顯減少.結論 BRMS1通過減少p-FAK/p-Src錶達抑製膠質瘤細胞黏附和遷移能力.
목적 탐토유선암전이억제기인1(BRMS1)재효질류세포점부화천이중적작용화궤제.방법 지질체개도진핵표체질립pEGFP-BRMS1화대조질립pEGFP-N2전입인효질류U251화U87세포중,Western blot기술검측BRMS1단백적표체.점부실험화천이실험검측세포점부화천이능력.Western blot검측린산화점착반격매(FAK) (p-FAK)화린산화락안산격매(Src)(p-Src)단백적표체.결과 여대조조비교,전염pEGFP-BRMS1질립24 h후U251화U87세포중BRMS1단백표체현저증고;세포점부능력분별하강60％화65％;세포천월Transwell소실능력분별하강64％화68％,차이균유통계학의의(P＜0.01);전염BRMS1후량충세포p-FAK화p-Src표체량명현감소.결론 BRMS1통과감소p-FAK/p-Src표체억제효질류세포점부화천이능력.
Objective To investigate the effect and mechanism of breast cancer metastasis suppressor 1 (BRMS1) on the invasion of human glioma cells.Methods The pEGFP-BRMS1 plasmids and pEGFP-N2 control plasmids were transfected into the U251 and U87 glioma cells using Lipofectamine 2000.The expression of BRMS1 mRNA and the expression levels of phosphorylated tyrosine kinase (Src) and focal adhesion kinase (FAK) proteins after BRMS1 restoration in both glioma cell lines were assessed by Western blotting.The roles of BRMS1 in the adhesion and migration of glioma cells were studied by using cell attachment assay and cell migration assay respectively.Results Compared to the control plasmids,pEGFP-BRMS1 plasmids could increase the expression of BRMS1 in both glioma cell lines significantly.Cell attachment assay revealed that BRMS1 inhibited the adhesion ability of U251 and U87 cells by 60％ and 63％,respectively (P ＜ 0.01).Cell migration assay showed that the migration ability of U251BRMS1 cells and U87-BRMS1 cells was decreased by 62％ and 68％ respectively as compared with the control cells (P ＜ 0.01).The phosphorylated Src and FAK expression levels were significantly suppressed after BRMS1 transfection into U251 and U87 cells as compared with the control cells.Conclusion BRMS1 may suppress the adhesion and migration ability of glioma cells by reducing the expression of phosphorylated FAK and Src.
