CAJ | 학술논문

目的观察转染视网膜母细胞瘤蛋白结合锌指结构基因1(RIZ1)后,食管鳞状细胞癌TE13细胞株基因表达谱的改变.方法培养TE13细胞株,1∶10传代后培养至对数生长期,脂质体法分别转染pcDNA3.1(+)和pcDNA3.1(+)/RIZ1真核表达载体,转染成功后,应用Agilent人类全基因表达谱基因芯片筛选转染前后的差异基因(差异倍数≥2.0或≤0.5),用聚合酶链反应(PCR)验证结果和对差异基因功能分析.结果差异基因总共有2123个,其中上调960个,下调1163个,功能主要集中在细胞发育、病毒复制的监管等.RIZ1基因可能参与包括细胞因子受体互相作用等多种信号通路.结论 RIZ1基因通过多种途径发挥作用,为进一步研究RIZ1基因与食管癌间的关系提供了依据.
목적 관찰전염시망막모세포류단백결합자지결구기인1(RIZ1)후,식관린상세포암TE13세포주기인표체보적개변.방법 배양TE13세포주,1∶10전대후배양지대수생장기,지질체법분별전염pcDNA3.1(+)화pcDNA3.1(+)/RIZ1진핵표체재체,전염성공후,응용Agilent인류전기인표체보기인심편사선전염전후적차이기인(차이배수≥2.0혹≤0.5),용취합매련반응(PCR)험증결과화대차이기인공능분석.결과 차이기인총공유2123개,기중상조960개,하조1163개,공능주요집중재세포발육、병독복제적감관등.RIZ1기인가능삼여포괄세포인자수체호상작용등다충신호통로.결론 RIZ1기인통과다충도경발휘작용,위진일보연구RIZ1기인여식관암간적관계제공료의거.
Objective To observe the effects of retinoblastoma protein-interacting zinc finger gene 1 (RIZ1) gene on the expression profile of esophageal squamous cell carcinoma cell line TE13.Methods The TE13 cells were cultured and divided into 10 portions,subcultured and transfected respectively by pcDNA3.1 (+) and pcDNA3.1 (+)/RIZ1 using the lipofectamin transfection reagent.After successful transfection,Agilent whole human genome oligo microarray (4 * 44K) was used to screen the effects of RIZ1 on the expression profile of esophageal carcinoma cell line TE13 (foldchange ≥2 or ≤0.5).The test results was confirmed by real-time quantitative polymerase chain reaction (real-time PCR).Results The DNA microarray data suggested that RIZ1 transfection induced widespread change of gene expression profile of TE13 cells.The up-regulated and down-regulated genes were amount to 960 and 1 163 respectively.The altered genes belonged to many functional categories,mainly in cell development,regulation of viral reproduction.RIZ1 gene may be involved in some pathways,such as cytokine receptor interaction,TGF-beta signaling pathway.Conclusion Widespread change of gene expression profile occurred after transfection with RIZ1 gene.We postulate that RIZ1 inhibits the tumor via the effect of these genes and involvement in pathways.The study provides the evidence for elucidating the relation between RIZ1 gene and the development and progress of esophageal cancer.