中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
12期
2640-2643
,共4页
薛才广%梁德刚%田轶魁%钱兆洋%杜鑫%魏民新
薛纔廣%樑德剛%田軼魁%錢兆洋%杜鑫%魏民新
설재엄%량덕강%전질괴%전조양%두흠%위민신
微小RNA-133a%心肌缺血再灌注损伤%心室重塑%细胞凋亡
微小RNA-133a%心肌缺血再灌註損傷%心室重塑%細胞凋亡
미소RNA-133a%심기결혈재관주손상%심실중소%세포조망
MicroRNA-133a%Myocardial ischemia and reperfusion%Ventricular remodeling%Cell apoptosis
目的 观察心肌缺血再灌注损伤后心室重塑的动态过程以及微小RNA(miRNA)-133a在此过程中的表达.方法 建立活体大鼠心肌缺血再灌注损伤模型.将实验动物随机分为假手术组(Sham组)、缺血再灌注损伤组(I/R组).选取术后1、3、7、15、30d 5个观察点,将每组分为5个亚组,监测心脏功能,检测心室重塑指标以及心肌组织内miRNA-133a的表达.结果 I/R组:随再灌注时间延长心功能逐渐下降,心肌内Ⅰ型胶原蛋白表达明显上调,心肌细胞凋亡率明显升高(P＜0.05),缺血区间质纤维化加重,心肌组织内miRNA-133a表达下调(P＜0.05);其中第3天,细胞凋亡率最高,为(54.6±7.3)％,纤维化开始明显[L/R组比Sham组:(5.60±2.30)％比(1.64±0.41)％,P＜0.05],心肌组织内miRNA-133a表达量最低(0.57±0.22).结论 心肌缺血再灌注损伤后发生纤维化重塑,再灌注后3d是心室重塑形成的重要时期;miRNA-133a在心肌缺血再灌注后心室重塑的动态过程中表达明显下调,可能参与调节此过程.
目的 觀察心肌缺血再灌註損傷後心室重塑的動態過程以及微小RNA(miRNA)-133a在此過程中的錶達.方法 建立活體大鼠心肌缺血再灌註損傷模型.將實驗動物隨機分為假手術組(Sham組)、缺血再灌註損傷組(I/R組).選取術後1、3、7、15、30d 5箇觀察點,將每組分為5箇亞組,鑑測心髒功能,檢測心室重塑指標以及心肌組織內miRNA-133a的錶達.結果 I/R組:隨再灌註時間延長心功能逐漸下降,心肌內Ⅰ型膠原蛋白錶達明顯上調,心肌細胞凋亡率明顯升高(P＜0.05),缺血區間質纖維化加重,心肌組織內miRNA-133a錶達下調(P＜0.05);其中第3天,細胞凋亡率最高,為(54.6±7.3)％,纖維化開始明顯[L/R組比Sham組:(5.60±2.30)％比(1.64±0.41)％,P＜0.05],心肌組織內miRNA-133a錶達量最低(0.57±0.22).結論 心肌缺血再灌註損傷後髮生纖維化重塑,再灌註後3d是心室重塑形成的重要時期;miRNA-133a在心肌缺血再灌註後心室重塑的動態過程中錶達明顯下調,可能參與調節此過程.
목적 관찰심기결혈재관주손상후심실중소적동태과정이급미소RNA(miRNA)-133a재차과정중적표체.방법 건립활체대서심기결혈재관주손상모형.장실험동물수궤분위가수술조(Sham조)、결혈재관주손상조(I/R조).선취술후1、3、7、15、30d 5개관찰점,장매조분위5개아조,감측심장공능,검측심실중소지표이급심기조직내miRNA-133a적표체.결과 I/R조:수재관주시간연장심공능축점하강,심기내Ⅰ형효원단백표체명현상조,심기세포조망솔명현승고(P＜0.05),결혈구간질섬유화가중,심기조직내miRNA-133a표체하조(P＜0.05);기중제3천,세포조망솔최고,위(54.6±7.3)％,섬유화개시명현[L/R조비Sham조:(5.60±2.30)％비(1.64±0.41)％,P＜0.05],심기조직내miRNA-133a표체량최저(0.57±0.22).결론 심기결혈재관주손상후발생섬유화중소,재관주후3d시심실중소형성적중요시기;miRNA-133a재심기결혈재관주후심실중소적동태과정중표체명현하조,가능삼여조절차과정.
Objective To observe the dynamic process of ventricular remodeling after myocardial ischemia-reperfusion injury,and to explore the expression of microRNA (miRNA)-133a in the dynamic process of ventricular remodeling.Methods The model of myocardial ischemia-reperfusion injury in rats was constructed.Healthy adult male wistar rats were randomly divided into two groups:(1) Sham group ; (2) I/R group.In postoperative 1,3,7,15,and 30d,cardiac function was analyzed,indicators of ventricular remodeling and miRNA-133a levels were detected.Results In L/R group:with reperfusion time extension,cardiac function was gradually declined.The expression of type I collagen were significantly higher.Apoptosis were significantly increased (P ＜ 0.05).Ischemia area myocardial fibrosis was gradually worsened.miRNA-133a expression levels were markedly lowered (P ＜ 0.05).On the 3 d after reperfusion,apoptosis rate was highest (54.6 ±-7.3) ％,Fibrosis became obvious [I/R VS Sham:(5.60 ± 2.30) ％ vs.(1.64 ± 0.41) ％,P ＜ 0.05],and miRNA-133a was the lowest (0.57 ± 0.22).Conclusion Fibrosis remodeling occurred after myocardial ischemia-reperfusion.An important period of ventricular remodeling was from 3d after reperfusion.The miRNA-133a expression was significantly lowered in the process of ventricular remodeling after myocardial ischemia-reperfusion injury.miRNA-133a may be involved in the regulation of myocardial ischemia and reperfusion ventricular remodeling process.
