CAJ | 학술논문

目的观察梯度神经生长因子(NGF)对背根神经节(DRG)轴突生长的引导及促进作用.方法制备聚左旋丙交酯(PLLA)膜,再使NGF梯度负载于该膜.取孕14 d胎鼠DRG,随机分为两组,A组(n=10)种植于负载有均匀NGF的膜上;B组(n=10)种植于负载有梯度NGF的膜上.培养5d后,测量轴突长度.结果梯度NGF组的DRG轴突长度为(1530±30).μm,轴突转弯系数(TR)为0.49 ±0.10;而均匀NGF组的DRG轴突长度为(1210±20)μm,TR为0.10±0.08.两组间的轴突长度与TR比较差异有统计学意义(P＜0.05).结论 NGF梯度信号对背根神经节轴突生长具有引导及促进作用.
목적 관찰제도신경생장인자(NGF)대배근신경절(DRG)축돌생장적인도급촉진작용.방법 제비취좌선병교지(PLLA)막,재사NGF제도부재우해막.취잉14 d태서DRG,수궤분위량조,A조(n=10)충식우부재유균균NGF적막상;B조(n=10)충식우부재유제도NGF적막상.배양5d후,측량축돌장도.결과 제도NGF조적DRG축돌장도위(1530±30).μm,축돌전만계수(TR)위0.49 ±0.10;이균균NGF조적DRG축돌장도위(1210±20)μm,TR위0.10±0.08.량조간적축돌장도여TR비교차이유통계학의의(P＜0.05).결론 NGF제도신호대배근신경절축돌생장구유인도급촉진작용.
Objective To investigate the effects of gradient nerve growth factor (NGF) on guiding and promoting the growth of dorsal root ganglia (DRG) neurite.Methods The poly L-lactic acid (PLLA) membranes were prepared,and then coated by gradient NGF.Twenty DRGs from El4 rats were randomly divided into two groups.The DRGs in group A (n =10) were placed on the gradient NGF immobilized membranes,and those in group B (n =10) were placed on the uniform NGF immobilized membranes.After 5 days of culture,the effects of gradient NGF signal on the growth of neurites of DRGs were evaluated by using immunofluorescence staining.Results The length of DRG neurite in gradient NGF group was 1530 ±30 μm,and that in uniform NGF group was 1210 ±20 μm.The neurite turning ratio was 0.49 ± 0.10 in gradient NGF group,and that was 0.10 ± 0.08 in uniform NGF group.Conclusion The NGF gradient signal could promote and guide the growth of DRG neurite.